MitoOx2: Difference between revisions

Latest revision as of 13:08, 12 February 2021

high-resolution terminology - matching measurements at high-resolution

MitoOx2

Description

Mitochondrial respiration medium, MitoOx2, developed for oxygraph incubations of mitochondrial preparations to measure the H₂O₂ production. MitoOx2 yields a higher optical sensitivity and lower "drift" (oxidation of the fluorophore precurcor without H₂O₂ present) for Amplex UltraRed(R) than e.g. MiR05.

Abbreviation: MitoOx2

MitoPedia topics: Media for respirometry

Application in HRR

MitoOx2: Mitochondrial Respiration Medium for measuring the H₂O₂ production

Oxygen solubility factor of MitoOx2 at 30°C and 37°C = 0.95

Chemicals for MitoOx2

Compound	Final conc.	M [g·mol^-1]	Company, product code and storage
KCl	120 mM	74.55	Sigma 31248, store at RT
HEPES free acid	20 mM	238.31	Sigma H-7523 store at RT
KH₂PO₄	10 mM	136.09	Sigma P-5655, store at RT
MgCl₂	2.86 mM	203.30	Scharlau MA0036, store at RT
EGTA free acid	0.2 mM	380.35	Sigma E-4378, store at RT
BSA essentially fatty acid free	0.025%	-	Sigma A-6003, store at 2 - 8 °C

adjust pH to 7.0 (room temperature) with KOH

@@ Line 1: / Line 1: @@
 {{MitoPedia
 |abbr=MitoOx2
-|description='''Mitochondrial respiration medium, MitoOx2''', developed for oxygraph incubations of [[mitochondrial preparations]] which yields a higher optical sensitivity for Amplex red.
+|description='''Mitochondrial respiration medium, MitoOx2''', developed for oxygraph incubations of [[mitochondrial preparations]] to measure the H<sub>2</sub>O<sub>2</sub> production. MitoOx2 yields a higher optical sensitivity and lower "drift" (oxidation of the fluorophore precurcor without H<sub>2</sub>O<sub>2</sub> present) for Amplex UltraRed(R) than e.g. [[MiR05|MiR05]].
 |type=Chemicals
 }}
-{{MitoPedia methods
+{{MitoPedia concepts|type=Chemicals
-|mitopedia method=Respirometry
+}}
-|type=Chemicals
+{{MitoPedia methods|type=Chemicals
+}}
+{{MitoPedia O2k and high-resolution respirometry|type=Chemicals
 }}
 {{MitoPedia topics
@@ Line 12: / Line 14: @@
 |type=Chemicals
 }}
+== Application in [[HRR]] ==
-== Application in [[HRR]] ==
+'''MitoOx2: Mitochondrial Respiration Medium for measuring the H<sub>2</sub>O<sub>2</sub> production'''
-'''MitoOx2: Mitochondrial Respiration Medium'''
+::Oxygen solubility factor of MitoOx2 at 30°C and 37°C = 0.95
-::Oxygen solubility factor of MitoOX2 at 30°C and 37°C = 0.95
-::pH of MitoOx2: 7.0
@@ Line 26: / Line 27: @@
 {| class="wikitable"
 |-
-! Compound !! Final conc. !! FW !! Company, product code and storage
+! Compound !! Final conc. !! M [g·mol<sup>-1</sup>] !! Company, product code and storage
 |-
-| KCl || 120 mM || 74.55 || |align="right"|Sigma 31248, store at RT
+| KCl || 120 mM || 74.55 || align="right" |Sigma 31248, store at RT
 |-
-| HEPES free acid || 20 mM || 238.31 || Sigma H-7523 store at RT
+| HEPES free acid || 20 mM || 238.31 || align="right" | Sigma H-7523 store at RT
 |-
-| KH<sub>2</sub>PO<sub>4</sub> || 10 mM || 136.09 || Sigma P-5655, store at RT
+| KH<sub>2</sub>PO<sub>4</sub> || 10 mM || 136.09 || align="right" | Sigma P-5655, store at RT
 |-
-| MgCl<sub>2</sub>|| 2.86 mM || 203.30 || Scharlau MA0036, store at RT
+| MgCl<sub>2</sub>|| 2.86 mM || 203.30 || align="right" | Scharlau MA0036, store at RT
 |-
-| EGTA free acid || 380.35 || 380.35 || Sigma E-4378, store at RT
+| EGTA free acid || 0.2 mM || 380.35 || align="right" | Sigma E-4378, store at RT
 |-
-| BSA essentially fatty acid free || 0.025% || - || Sigma A-6003, store at 2 - 8 °C
+| BSA essentially fatty acid free || 0.025% || - || align="right" | Sigma A-6003, store at 2 - 8 °C
 |}
+adjust pH to 7.0 (room temperature) with KOH