MiRK03: Difference between revisions
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{{MitoPedia | {{MitoPedia | ||
|abbr=MiRK03 | |abbr=MiRK03 | ||
|description='''Mitochondrial respiration medium, | |description='''Mitochondrial respiration medium, MiRK03''', modified after a medium described by [[Komary 2010 Biochim Biophys Acta]], intended for use as medium for H2O2 production measurement with Amplex Red. | ||
|info=[http://bioblast.at/index.php/Krumschnabel_2014_Methods_Mol_Biol Krumschnabel 2014 Methods Mol Biol], [http://bioblast.at/index.php/Komary_2010_Biochim_Biophys_Acta Komary 2010 Biochim Biophys Acta] | |info=[http://bioblast.at/index.php/Krumschnabel_2014_Methods_Mol_Biol Krumschnabel 2014 Methods Mol Biol], [http://bioblast.at/index.php/Komary_2010_Biochim_Biophys_Acta Komary 2010 Biochim Biophys Acta] | ||
|type=Chemicals | |type=Chemicals | ||
| Line 33: | Line 33: | ||
| BSA || 1 mg/ml || - || 1000 mg | | BSA || 1 mg/ml || - || 1000 mg | ||
|} | |} | ||
==== Preparation without BSA ==== | |||
::::# Weigh all ingredients (besides BSA) according to the table above and transfer them into a big glass beaker. | |||
::::# Add about 750 mL H<sub>2</sub>O. | |||
::::# Under stirring adjust the temperature to 25 °C. | |||
::::# Adjust the pH to 7.12 with 5 M KOH. | |||
::::# Adjust final volume to 1 L and divide in 40 mL portions. | |||
::::# Store at -20 °C. | |||
==== MiRK03 ph - temperature dependence ==== | |||
data recorded with ''MiRK03 without BSA'' (only averaged values are shown; two measurements were conducted) | data recorded with ''MiRK03 without BSA'' (only averaged values are shown; two measurements were conducted) | ||
Latest revision as of 10:01, 9 November 2020
- high-resolution terminology - matching measurements at high-resolution
MiRK03
Description
Mitochondrial respiration medium, MiRK03, modified after a medium described by Komary 2010 Biochim Biophys Acta, intended for use as medium for H2O2 production measurement with Amplex Red.
Abbreviation: MiRK03
Reference: Krumschnabel 2014 Methods Mol Biol, Komary 2010 Biochim Biophys Acta
MitoPedia topics: Media for respirometry
MiRK03
| substance | MiRK03 | molecular weight | for 1 l |
|---|---|---|---|
| EGTA | 0.5 mM | 380.35 g/mol | 190.18 mg |
| MgCl2 x 6 H2O | 3 mM | 203.30 g/mol | 609.90 mg |
| HEPES | 20 mM | 238.31 g/mol | 4 766.2 mg |
| KCl | 130 mM | 74.56 g/mol | 9 692.8 mg |
| KH2PO4 | 10 mM | 136.09 g/mol | 1 360.9 mg |
| BSA | 1 mg/ml | - | 1000 mg |
Preparation without BSA
- Weigh all ingredients (besides BSA) according to the table above and transfer them into a big glass beaker.
- Add about 750 mL H2O.
- Under stirring adjust the temperature to 25 °C.
- Adjust the pH to 7.12 with 5 M KOH.
- Adjust final volume to 1 L and divide in 40 mL portions.
- Store at -20 °C.
MiRK03 ph - temperature dependence
data recorded with MiRK03 without BSA (only averaged values are shown; two measurements were conducted)
| temperature [°C] | pH of MiRK03 |
|---|---|
| 20 | 7.15 |
| 25 | 7.12 |
| 30 | 7.09 |
| 35 | 7.05 |
| 37 | 7.03 |
- For comparison see: Mitochondrial respiration media: comparison
