MiPNet08.12 IOC22: Difference between revisions
Beno Marija (talk | contribs) m (Beno Marija moved page MiPNet08.12 NO-O2kWorkshop to MiPNet08.12 IOC22) |
No edit summary |
||
Line 14: | Line 14: | ||
[[Aguirre_2010_Biochim_Biophys_Acta| Reference: Biochim Biophys Acta 1797: 557-565 (2010)]] | [[Aguirre_2010_Biochim_Biophys_Acta| Reference: Biochim Biophys Acta 1797: 557-565 (2010)]] | ||
:ยป Product: [[ | :ยป Product: [[Oroboros O2k]], [[OROBOROS O2k-Catalogue | O2k-Catalogue]] | ||
|mipnetlab=AT_Innsbruck_OROBOROS, US_AL Birmingham_Kraus DW | |mipnetlab=AT_Innsbruck_OROBOROS, US_AL Birmingham_Kraus DW | ||
}} | }} |
Revision as of 19:31, 8 July 2017
MiPNet08.12 IOC22
NO effect on mitochondrial oxygen kinetics at low oxygen. O2k workshop Report. |
OROBOROS (2011-12-11) Mitochondr Physiol Network
Abstract: Gnaiger E, Doeller JE, Kraus D, Shiva S, Brookes PS, Darley-Usmar VM (2011) NO effect on mitochondrial oxygen kinetics at low oxygen. O2k workshop Report. Mitochondr Physiol Network 08.12(07). ยปVersions
A single pilot experiment was carried out during an O2k workshop on high-resolution respirometry (IOC22). Respiration of isolated rat liver mitochondria was inhibited by addition of NO, which increased the sensitivity to oxygen >25-fold when compared to the half-saturation oxygen pressure, p50, in the absence of NO. Oxygen kinetics followed a monophasic hyperbolic function up to 2.2 kPa with NO (p50=0.93 kPa), compared to the standard oxygen range to 1.1 kPa without NO (p50=0.035 kPa).
Reference: Biochim Biophys Acta 1797: 557-565 (2010)
- ยป Product: Oroboros O2k, O2k-Catalogue
โข O2k-Network Lab: AT_Innsbruck_OROBOROS, US_AL Birmingham_Kraus DW
Labels: MiParea: Respiration, Instruments;methods
Stress:Oxidative stress;RONS Organism: Rat Tissue;cell: Liver Preparation: Isolated mitochondria
Regulation: Inhibitor, Oxygen kinetics Coupling state: OXPHOS
HRR: Oxygraph-2k, NO, O2k-Protocol
O2k-Demo, O2k-MultiSensor