MiPNet03.02 Chemicals-Media: Difference between revisions
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|year=2013 | |year=2013 | ||
|journal=Mitochondr Physiol Network | |journal=Mitochondr Physiol Network | ||
|abstract=Different media for tissue preparation and respiration are used in investigations of mitochondrial function. Initial decisions on the composition of media and chemicals are decisive for long-term studies and crucial for comparability of results. As a guideline, we summarize an update of our experience with media and chemicals for high-resolution respirometry with isolated mitochondria, permeabilized cells, muscle fibres and tissue homogenates. Whereas optimization is necessary for specific experimental protocols, standardization will improve the comparability of data obtained in different laboratories. Such efforts towards standardization are important for the advancement of mitochondrial physiology and mitochondrial medicine. | |abstract='''O2k-Protocol for chemicals and media''' - [http://www.oroboros.at/?o2k-protocols O2k-Protocols contents] | ||
Different media for tissue preparation and respiration are used in investigations of mitochondrial function. Initial decisions on the composition of media and chemicals are decisive for long-term studies and crucial for comparability of results. As a guideline, we summarize an update of our experience with media and chemicals for high-resolution respirometry with isolated mitochondria, permeabilized cells, muscle fibres and tissue homogenates. Whereas optimization is necessary for specific experimental protocols, standardization will improve the comparability of data obtained in different laboratories. Such efforts towards standardization are important for the advancement of mitochondrial physiology and mitochondrial medicine. | |||
|mipnetlab=AT Innsbruck OROBOROS, | |mipnetlab=AT Innsbruck OROBOROS, | ||
|articletype=Protocol; Manual, MiPNet-online Publication | |articletype=Protocol; Manual, MiPNet-online Publication | ||
Revision as of 15:22, 16 October 2013
| Fontana-Ayoub M, Eigentler A, Fasching M, Gnaiger E (2013) Selected media and chemicals for respirometry with mitochondrial preparations. Mitochondr Physiol Network 03.02. |
» Bioblast File:MiPNet03.02 Chemicals-Media.pdf, MiPNet03.02
OROBOROS (2013) Mitochondr Physiol Network
Abstract: O2k-Protocol for chemicals and media - O2k-Protocols contents
Different media for tissue preparation and respiration are used in investigations of mitochondrial function. Initial decisions on the composition of media and chemicals are decisive for long-term studies and crucial for comparability of results. As a guideline, we summarize an update of our experience with media and chemicals for high-resolution respirometry with isolated mitochondria, permeabilized cells, muscle fibres and tissue homogenates. Whereas optimization is necessary for specific experimental protocols, standardization will improve the comparability of data obtained in different laboratories. Such efforts towards standardization are important for the advancement of mitochondrial physiology and mitochondrial medicine.
• O2k-Network Lab: AT Innsbruck OROBOROS
Labels: MiParea: Instruments;methods
HRR: Protocol"Protocol" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property.
Chemicals & Media
Chemical storage
Aliquots of stocks for rotenone, succinate, glutamate, malate, and oligomycin can be refrozen for later use, since these chemicals are stable.
