Malonate: Difference between revisions
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::1) Weigh 20.8 mg malonic acid into a Eppendorf tube of appropriate volume (for final volume of 100 µl). | ::1) Weigh 20.8 mg malonic acid into a Eppendorf tube of appropriate volume (for final volume of 100 µl). | ||
::2) Dissolve in | ::2) Dissolve in 85 µl 5 N KOH and check pH. | ||
::3) Titrate small volumes (2 µl) of 5 N KOH until you reach a pH of 6.0 (pH of 7.0 would be ideal, however the pH jumps from pH 6.0 to pH 10.0 with only very small excess KOH). | ::3) Titrate small volumes (2 µl) of 5 N KOH until you reach a pH of 6.0 (pH of 7.0 would be ideal, however the pH jumps from pH 6.0 to pH 10.0 with only very small excess KOH). | ||
::4) Add H<sub>2</sub>O to a final volume of 100 µl. | ::4) Add H<sub>2</sub>O to a final volume of 100 µl. | ||
Revision as of 12:03, 12 February 2015
- high-resolution terminology - matching measurements at high-resolution
Malonate
Description
Malonate (malonic acid) is a competitive inhibitor of Succinate dehydrogenase (Complex II).
Abbreviation: Mna
Reference: MiPNet09.12
MitoPedia topics: Inhibitor
Application in HRR
Mna: Malonic acid ( CH2(COOH)2); Sigma M 1296, 5 g, store at R.T.; FW = 104.06.
Recommendation: Prepare fresh everyday!
Preparation of 2 M stock solution (dissolved in H20):
- 1) Weigh 20.8 mg malonic acid into a Eppendorf tube of appropriate volume (for final volume of 100 µl).
- 2) Dissolve in 85 µl 5 N KOH and check pH.
- 3) Titrate small volumes (2 µl) of 5 N KOH until you reach a pH of 6.0 (pH of 7.0 would be ideal, however the pH jumps from pH 6.0 to pH 10.0 with only very small excess KOH).
- 4) Add H2O to a final volume of 100 µl.
Oxygraph-2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume: 5 µl using a 25 µl syringe (2 ml O2k-chamber).
- Final concentration: 5 mM.
