Malate: Difference between revisions
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|description=Mitochondrial respiration depends on a continuous flow of substrates across the mitochondrial membranes into the matrix space. [[Glutamate]] and '''malate''' are anions which cannot permeate through the lipid bilayer of membranes and hence require carriers, which is also true for pyruvate. Malate alone cannot support respiration of mt-preparations. | |description=Mitochondrial respiration depends on a continuous flow of substrates across the mitochondrial membranes into the matrix space. [[Glutamate]] and '''malate''' are anions which cannot permeate through the lipid bilayer of membranes and hence require carriers, which is also true for pyruvate. Malate alone cannot support respiration of mt-preparations. | ||
[[Malate dehydrogenase|Malate dehydrogenase]]located in the mitochondrial matrix oxidizes malate to oxaloacetate. | [[Malate dehydrogenase|Malate dehydrogenase]] located in the mitochondrial matrix oxidizes malate to oxaloacetate. | ||
|info=[[MiPNet11.04]]; [[MiPNet09.12]] | |info=[[MiPNet11.04]]; [[MiPNet09.12]] | ||
|type=Respiration | |type=Respiration | ||
Revision as of 08:42, 16 May 2012
- high-resolution terminology - matching measurements at high-resolution
Malate
Description
Mitochondrial respiration depends on a continuous flow of substrates across the mitochondrial membranes into the matrix space. Glutamate and malate are anions which cannot permeate through the lipid bilayer of membranes and hence require carriers, which is also true for pyruvate. Malate alone cannot support respiration of mt-preparations. Malate dehydrogenase located in the mitochondrial matrix oxidizes malate to oxaloacetate.
Abbreviation: M
Reference: MiPNet11.04; MiPNet09.12
MitoPedia topics:
Substrate and metabolite
Application in HRR
M: Malate (L-Malic acid, C4H605); Sigma M 1000, 100 g, store at R.T.: FW = 134.1)
Preparation of 800 mM stock solution (dissolved in H2O):
- 1) weigh 536.5 mg of L-Malic acid
- 2) add 3 ml H2O
- 3) neutralize with 10 N KOH (approx. 750 µl)
- 4) adjust final volume to 5 ml (in 5 mL volumetric glass flask)
- 5) divide into 0.2 ml portions
- 6) store frozen at -20 °C
Oxygraph-2k Manual Titrations: MiPNet09.12
- Titration volume: 5 µl using a 25 µl Hamilton syringe
- Final conc. in 2 ml O2k-chamber: 2 mM
