Lehr 2021 Methods Mol Biol: Difference between revisions
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{{Publication | {{Publication | ||
|title=Lehr S, Hartwig S, Kotzka J (2021) Preparation of "functional" mitochondria: a challenging business.ย https://doi.org/10.1007/978-1-0716-1266-8_2 | |title=Lehr S, Hartwig S, Kotzka J (2021) Preparation of "functional" mitochondria: a challenging business.ย https://doi.org/10.1007/978-1-0716-1266-8_2 | ||
|info=Methods Mol Biol 2276:31-39. [https://www.ncbi.nlm.nih.gov/pubmed/34060030 PMID: 34060030 | |info=Methods Mol Biol 2276:31-39. [https://www.ncbi.nlm.nih.gov/pubmed/34060030 PMID: 34060030] | ||
|authors=Lehr Stefan, Hartwig Sonja, Kotzka Jorg | |authors=Lehr Stefan, Hartwig Sonja, Kotzka Jorg | ||
|year=2021 | |year=2021 | ||
|journal=Methods Mol Biol | |journal=Methods Mol Biol | ||
|abstract=As the powerhouse of the cell, mitochondria, plays a crucial role in many aspects of life, whereby mitochondrial dysfunctions are associated with pathogenesis of many diseases, like neurodegenerative diseases, obesity, cancer, and metabolic as well as cardiovascular disorders. Mitochondria analysis frequently starts with isolation and enrichment procedures, which have become increasingly important in biomedical research. Unfortunately, isolation procedures can easily cause changes in the structural integrity of mitochondria during ''in vitro'' handling having impact on their function. This carries the risk that conclusions about isolated mitochondria may be drawn on the basis of experimental artifacts. Here we critically review a commonly used isolation procedure for mitochondria utilizing differential (gradient) centrifugation and depict major challenges to achieve "functional" mitochondria as basis for comprehensive physiological studies. | |abstract=As the powerhouse of the cell, mitochondria, plays a crucial role in many aspects of life, whereby mitochondrial dysfunctions are associated with pathogenesis of many diseases, like neurodegenerative diseases, obesity, cancer, and metabolic as well as cardiovascular disorders. Mitochondria analysis frequently starts with isolation and enrichment procedures, which have become increasingly important in biomedical research. Unfortunately, isolation procedures can easily cause changes in the structural integrity of mitochondria during ''in vitro'' handling having impact on their function. This carries the risk that conclusions about isolated mitochondria may be drawn on the basis of experimental artifacts. Here we critically review a commonly used isolation procedure for mitochondria utilizing differential (gradient) centrifugation and depict major challenges to achieve "functional" mitochondria as basis for comprehensive physiological studies. | ||
|keywords=Differential gradient centrifugation, Isolation of mitochondria, Mitochondrial integrity | |||
|editor=[[Plangger M]] | |editor=[[Plangger M]] | ||
}} | }} | ||
{{Labeling | {{Labeling | ||
|area=Instruments;methods | |||
|preparations=Isolated mitochondria | |||
|additional=2023-04 | |additional=2023-04 | ||
}} | }} |
Latest revision as of 17:28, 24 April 2023
Lehr S, Hartwig S, Kotzka J (2021) Preparation of "functional" mitochondria: a challenging business. https://doi.org/10.1007/978-1-0716-1266-8_2 |
ยป Methods Mol Biol 2276:31-39. PMID: 34060030
Lehr Stefan, Hartwig Sonja, Kotzka Jorg (2021) Methods Mol Biol
Abstract: As the powerhouse of the cell, mitochondria, plays a crucial role in many aspects of life, whereby mitochondrial dysfunctions are associated with pathogenesis of many diseases, like neurodegenerative diseases, obesity, cancer, and metabolic as well as cardiovascular disorders. Mitochondria analysis frequently starts with isolation and enrichment procedures, which have become increasingly important in biomedical research. Unfortunately, isolation procedures can easily cause changes in the structural integrity of mitochondria during in vitro handling having impact on their function. This carries the risk that conclusions about isolated mitochondria may be drawn on the basis of experimental artifacts. Here we critically review a commonly used isolation procedure for mitochondria utilizing differential (gradient) centrifugation and depict major challenges to achieve "functional" mitochondria as basis for comprehensive physiological studies. โข Keywords: Differential gradient centrifugation, Isolation of mitochondria, Mitochondrial integrity โข Bioblast editor: Plangger M
Labels: MiParea: Instruments;methods
Preparation: Isolated mitochondria
2023-04