Labajova 2006 Gen Physiol Biophys: Difference between revisions
No edit summary |
No edit summary |
||
Line 5: | Line 5: | ||
|journal=General Physiology and Biophysics | |journal=General Physiology and Biophysics | ||
|abstract=The changes in mitochondrial membrane potential (ΞΟm) were used as | |abstract=The changes in mitochondrial membrane potential (ΞΟm) were used as | ||
an indicator for evaluating the mitochondrial permeability transition pore (MPTP) function. We found that in | an indicator for evaluating the mitochondrial permeability transition pore (MPTP) function. We found that ''in sit''u mitochondria in digitonin-permeabilized hepatocytes were coupled and responded to the addition of substrates, inhibitors and uncouplers. Ca<sup>2+</sup>-induced ΞΟm dissipation was caused by MPTP opening because this process was inhibited by cyclosporin A. MPTP opening was enhanced by the pro-oxidant tert-butyl hydroperoxide. | ||
|keywords=Tetraphenylphosphonium-selective electrode,Β Mitochondrial permeability transition pore,Β Hepatocytes | |keywords=Tetraphenylphosphonium-selective electrode,Β Mitochondrial permeability transition pore,Β Hepatocytes | ||
|info=[http://www.ncbi.nlm.nih.gov/pubmed/17197730 PMID: 17197730] | |info=[http://www.ncbi.nlm.nih.gov/pubmed/17197730 PMID: 17197730] |
Revision as of 14:10, 30 September 2010
LΓ‘bajovΓ‘ A, KofrΓ‘nek J, KrivΓ‘kovΓ‘ P, CervinkovΓ‘ Z, Drahota Z (2006) Tetraphenylphosphonium-Selective Electrode as a Tool for Evaluating Mitochondrial Permeability Transition Pore Function in Isolated Rat Hepatocytes. Gen. Physiol. Biophys. 25: 325β331. |
Labajova A, Kofranek J, Krivakova P, Cervinkova Z, Drahota Z (2006) General Physiology and Biophysics
Abstract: The changes in mitochondrial membrane potential (ΞΟm) were used as an indicator for evaluating the mitochondrial permeability transition pore (MPTP) function. We found that in situ mitochondria in digitonin-permeabilized hepatocytes were coupled and responded to the addition of substrates, inhibitors and uncouplers. Ca2+-induced ΞΟm dissipation was caused by MPTP opening because this process was inhibited by cyclosporin A. MPTP opening was enhanced by the pro-oxidant tert-butyl hydroperoxide. β’ Keywords: Tetraphenylphosphonium-selective electrode, Mitochondrial permeability transition pore, Hepatocytes
Labels:
Organism: Rat
Tissue;cell: Hepatocyte; Liver"Hepatocyte; Liver" is not in the list (Heart, Skeletal muscle, Nervous system, Liver, Kidney, Lung;gill, Islet cell;pancreas;thymus, Endothelial;epithelial;mesothelial cell, Blood cells, Fat, ...) of allowed values for the "Tissue and cell" property.
Regulation: Respiration; OXPHOS; ETS Capacity"Respiration; OXPHOS; ETS Capacity" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property., Coupling; Membrane Potential"Coupling; Membrane Potential" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property.
HRR: Oxygraph-2k