Labajova 2006 Gen Physiol Biophys: Difference between revisions
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{{Publication | {{Publication | ||
|title=Lábajová A, Kofránek J, Kriváková P, Cervinková Z, Drahota Z (2006) Tetraphenylphosphonium-Selective Electrode as a Tool for Evaluating Mitochondrial Permeability Transition Pore Function in Isolated Rat Hepatocytes. Gen. Physiol. Biophys. 25: 325—331. | |title=Lábajová A, Kofránek J, Kriváková P, Cervinková Z, Drahota Z (2006) Tetraphenylphosphonium-Selective Electrode as a Tool for Evaluating Mitochondrial Permeability Transition Pore Function in Isolated Rat Hepatocytes. Gen. Physiol. Biophys. 25: 325—331. | ||
|authors=Labajova A, Kofranek J, Krivakova P, Cervinkova Z, Drahota Z | |authors=Labajova A, Kofranek J, Krivakova P, Cervinkova Z, Drahota Z | ||
|year=2006 | |year=2006 | ||
|journal=General Physiology and Biophysics | |journal=General Physiology and Biophysics | ||
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{{Labeling | {{Labeling | ||
|discipline=Mitochondrial Physiology | |||
|organism=Rat | |organism=Rat | ||
|tissues=Hepatocyte; Liver | |tissues=Hepatocyte; Liver | ||
|topics=Respiration; OXPHOS; ETS Capacity | |topics=Respiration; OXPHOS; ETS Capacity, Coupling; Membrane Potential | ||
|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
|articletype=Protocol; Manual | |||
}} | }} | ||
Revision as of 14:09, 30 September 2010
| Lábajová A, Kofránek J, Kriváková P, Cervinková Z, Drahota Z (2006) Tetraphenylphosphonium-Selective Electrode as a Tool for Evaluating Mitochondrial Permeability Transition Pore Function in Isolated Rat Hepatocytes. Gen. Physiol. Biophys. 25: 325—331. |
Labajova A, Kofranek J, Krivakova P, Cervinkova Z, Drahota Z (2006) General Physiology and Biophysics
Abstract: The changes in mitochondrial membrane potential (Δψm) were used as an indicator for evaluating the mitochondrial permeability transition pore (MPTP) function. We found that in situ mitochondria in digitonin-permeabilized hepatocytes were coupled and responded to the addition of substrates, inhibitors and uncouplers. Ca2+-induced Δψm dissipation was caused by MPTP opening because this process was inhibited by cyclosporin A. MPTP opening was enhanced by the pro-oxidant tert-butyl hydroperoxide. • Keywords: Tetraphenylphosphonium-selective electrode, Mitochondrial permeability transition pore, Hepatocytes
Labels:
Organism: Rat
Tissue;cell: Hepatocyte; Liver"Hepatocyte; Liver" is not in the list (Heart, Skeletal muscle, Nervous system, Liver, Kidney, Lung;gill, Islet cell;pancreas;thymus, Endothelial;epithelial;mesothelial cell, Blood cells, Fat, ...) of allowed values for the "Tissue and cell" property.
Regulation: Respiration; OXPHOS; ETS Capacity"Respiration; OXPHOS; ETS Capacity" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property., Coupling; Membrane Potential"Coupling; Membrane Potential" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property.
HRR: Oxygraph-2k
