Description
Glycerophosphate (synonym: Ξ±-glycerophosphate; glycerol-3-phosphate; C3H9O6P) is an organophosphate and it is a component of glycerophospholipids. The mitochondrial Glycerophosphate dehydrogenase complex oxidizes glycerophosphate to dihydroxyacetone phosphate and feeds electrons directly to ubiquinone.
Abbreviation: Gp
MitoPedia topics: Substrate and metabolite
Nomenclature
Due to an ambiguity in the D,L nomenclature based on the Fisher projection the biologically active enantiomer can be labeled either D-glycerol 1-phosphate or L-glycerol 3-phosphate. Alternatives are sn-glycerol 3-phosphate or DL-alpha-glycerophosphate. Following the CahnβIngoldβPrelog priority rules, the biologically active substance is always the (R) enantiomer, independent of whether the substance is called glycerol 1-phosphate or glycerol 3-phosphate.
Source
Recommendation by Chuck Hoppel, Director, Ctr Mitochondrial Diseases; Professor, Dept Pharmacology, Case Western Reserve Univ, Cleveland, Ohio - US OH Cleveland Hoppel CL:
- 94124 Sigma sn-Glycerol 3-phosphate lithium salt, β₯95.0% (TLC) Synonym: D-Glycerol 1-phosphate lithium salt, L-Glycerol 3-phosphate lithium salt. MSDS. CAS Number 17989-41-2 (free acid) Empirical Formula (Hill Notation) C3H9O6P Β· xLi+, Molecular Weight 172.07 (free acid basis).
Application in HRR
Gp: Glycerol 3-phosphate (sn-Glycerol 3-phosphate lithium salt, C3H9O6P β’ xLi+); Sigma 94124, store at -20 Β°C; FW = 172.07 (free acid basis).
Preparation of 1 M stock solution (dissolved in H2O):
- 1. Weigh 860.35 mg of Gp and dissolve in 3 ml H2O.
- 2. Check pH and adjust to 7.0 if necessary with 1 N HCl.
- 3. Transfer to 5 ml volumetric glass flask and adjust the final volume to 5 ml.
- 4. Divide into 0.5 ml portions.
- 5. Store frozen at -20 Β°C.
Oxygraph-2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume: 20 Β΅l using a 50 Β΅l syringe (2 ml O2k-chamber).
- Final concentration: 10 mM.
- 1 M stock solution, final concentration in the O2k-Chamber is 10 mM.
If a racemic mixture (like Sigma G-2138) is replaced by the biologically active enantiomer the amount of substance (measured in moles) usually has to be halved. However, also consider that stated purities may vary, requiring further adjustments.