Glycerophosphate: Difference between revisions
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== Application in HRR == | == Application in HRR == | ||
'''Gp: Glycerol 3-phosphate ''' (sn-Glycerol 3-phosphate lithium salt, C<sub>3</sub>H<sub>9</sub>O<sub>6</sub>P β’ xLi+); Sigma 94124, store at -20 Β°C; FW = 172.07 (free acid basis). | |||
'''Preparation of 1 M stock solution''' (dissolved in H<sub>2</sub>O): | |||
::1. Weigh 860.35 mg of Gp and dissolve in 3 ml H<sub>2</sub>O. | |||
::2. Check pH and adjust to 7.0 if necessary with 1 N HCl. | |||
::3. Transfer to 5 ml volumetric glass flask and adjust the final volume to 5 ml. | |||
::4. Divide into 0.5 ml portions. | |||
::5. Store frozen at -20 Β°C. | |||
'''Oxygraph-2k manual titrations''' [[MiPNet09.12 O2k-Titrations]] | |||
::* Titration volume: 20 Β΅l using a 50 Β΅l syringe (2 ml O2k-chamber). | |||
::* Final concentration: 10 mM. | |||
:* 1 M stock solution, final concentration in the O2k-Chamber is 10 mM. Β | :* 1 M stock solution, final concentration in the O2k-Chamber is 10 mM. Β | ||
If a racemic mixture (like Sigma G-2138) is replaced by the biologically active enantiomer the amount of substance (measured in moles) usually has to be halved. However, also consider that stated purities may vary, requiring further adjustments. | If a racemic mixture (like Sigma G-2138) is replaced by the biologically active enantiomer the amount of substance (measured in moles) usually has to be halved. However, also consider that stated purities may vary, requiring further adjustments. | ||
Revision as of 12:07, 16 June 2016
Description
Glycerophosphate (synonym: Ξ±-glycerophosphate; glycerol-3-phosphate; C3H9O6P) is an organophosphate and it is a component of glycerophospholipids. The mitochondrial Glycerophosphate dehydrogenase complex oxidizes glycerophosphate to dihydroxyacetone phosphate and feeds electrons directly to ubiquinone.
Abbreviation: Gp
MitoPedia topics: Substrate and metabolite
Nomenclature
Due to an ambiguity in the D,L nomenclature based on the Fisher projection the biologically active enantiomer can be labeled either D-glycerol 1-phosphate or L-glycerol 3-phosphate. Alternatives are sn-glycerol 3-phosphate or DL-alpha-glycerophosphate. Following the CahnβIngoldβPrelog priority rules, the biologically active substance is always the (R) enantiomer, independent of whether the substance is called glycerol 1-phosphate or glycerol 3-phosphate.
Source
Recommendation by Chuck Hoppel, Director, Ctr Mitochondrial Diseases; Professor, Dept Pharmacology, Case Western Reserve Univ, Cleveland, Ohio - US OH Cleveland Hoppel CL:
- 94124 Sigma sn-Glycerol 3-phosphate lithium salt, β₯95.0% (TLC) Synonym: D-Glycerol 1-phosphate lithium salt, L-Glycerol 3-phosphate lithium salt. MSDS. CAS Number 17989-41-2 (free acid) Empirical Formula (Hill Notation) C3H9O6P Β· xLi+, Molecular Weight 172.07 (free acid basis).
Application in HRR
Gp: Glycerol 3-phosphate (sn-Glycerol 3-phosphate lithium salt, C3H9O6P β’ xLi+); Sigma 94124, store at -20 Β°C; FW = 172.07 (free acid basis).
Preparation of 1 M stock solution (dissolved in H2O):
- 1. Weigh 860.35 mg of Gp and dissolve in 3 ml H2O.
- 2. Check pH and adjust to 7.0 if necessary with 1 N HCl.
- 3. Transfer to 5 ml volumetric glass flask and adjust the final volume to 5 ml.
- 4. Divide into 0.5 ml portions.
- 5. Store frozen at -20 Β°C.
Oxygraph-2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume: 20 Β΅l using a 50 Β΅l syringe (2 ml O2k-chamber).
- Final concentration: 10 mM.
- 1 M stock solution, final concentration in the O2k-Chamber is 10 mM.
If a racemic mixture (like Sigma G-2138) is replaced by the biologically active enantiomer the amount of substance (measured in moles) usually has to be halved. However, also consider that stated purities may vary, requiring further adjustments.