Dias 2018 Anal Biochem: Difference between revisions
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{{Publication | {{Publication | ||
|title=Dias C, Lourenco CF, Barbosa RM, Laranjinha J, Ledo A (2018) Analysis of respiratory capacity in brain tissue preparations: high-resolution respirometry for intact hippocampal slices. Anal Biochem 551:43-50. | |title=Dias C, Lourenco CF, Barbosa RM, Laranjinha J, Ledo A (2018) Analysis of respiratory capacity in brain tissue preparations: high-resolution respirometry for intact hippocampal slices. Anal Biochem 551:43-50. | ||
|info=[https://www.ncbi.nlm.nih.gov/pubmed/29753719 PMID: 29753719] | |info=[https://www.ncbi.nlm.nih.gov/pubmed/29753719 PMID: 29753719]ยป[[File:O2k-brief.png|36px|link=https://wiki.oroboros.at/images/9/95/Dias_2018_Analyt_Biochem_O2k-brief.pdf|O2k-brief]] | ||
|authors=Dias C, Lourenco CF, Barbosa RM, Laranjinha J, Ledo AM | |authors=Dias C, Lourenco CF, Barbosa RM, Laranjinha J, Ledo AM | ||
|year=2018 | |year=2018 |
Revision as of 13:01, 11 November 2019
Dias C, Lourenco CF, Barbosa RM, Laranjinha J, Ledo A (2018) Analysis of respiratory capacity in brain tissue preparations: high-resolution respirometry for intact hippocampal slices. Anal Biochem 551:43-50. |
ยป PMID: 29753719ยป
Dias C, Lourenco CF, Barbosa RM, Laranjinha J, Ledo AM (2018) Anal Biochem
Abstract: The evaluation of mitochondrial function provides the basis for the study of brain bioenergetics. However, analysis of brain mitochondrial respiration has been hindered by the low yield associated with mitochondria isolation procedures. Furthermore, isolating mitochondria or cells results in loss of the inherent complexity of the central nervous system. High-resolution respirometry (HRR), is a valuable tool to study mitochondrial function and has been used in diverse biological preparations ranging from isolated mitochondria to tissue homogenates and permeabilized tissue biopsies. Here we describe a novel methodology for evaluation of mitochondrial respiration using tissue preparations from the central nervous system, namely acute hippocampal slices from rodents, with HRR. By using acute intact hippocampal slices, tissue cytoarchitecture, intercellular communication and connectivity are preserved. Mitochondrial respiration was evaluated by using an adapted substrate-uncoupler-inhibitor titration (SUIT) protocol and the expected responses were observed. This methodology can be used to detect differences in mitochondrial function at the oxidative phosphorylation level and for studies with different brain oxidative substrates in physiological and neuropathological settings, by using a system that better represents the in vivo conditions than isolated mitochondria and/or cells. โข Keywords: High-resolution respirometry, Oxygen consumption rate, Whole hippocampal slices, Bioenergetics, Oxidative phosphorylation โข Bioblast editor: Krumschnabel G โข O2k-Network Lab: PT Coimbra Laranjinha J
Labels: MiParea: Respiration, Instruments;methods
Organism: Mouse, Rat
Tissue;cell: Nervous system
Preparation: Intact cells
Coupling state: LEAK, ROUTINE, ET
Pathway: ROX
HRR: Oxygraph-2k
Tissue slice, 2018-07