Cyanide: Difference between revisions
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== Application in [[HRR]] == | == Application in [[HRR]] == | ||
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::::* [[alternative oxidase]] | ::::* [[alternative oxidase]] | ||
::: '''O2k manual titrations''' [[MiPNet09.12 O2k-Titrations]] | |||
ย | ::::* Titration volume: 2 ยตl of 1 M stock, using a 10 ยตl syringe (2 ml O2k-chamber). | ||
:::: | ::::* Final concentration: 1 mM. | ||
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=== Preparation of 1 M KCN stock === | |||
::: | :::: '''Kcn'''(KCN): Fluka 60178, 100 g, store at R.T.; FW = 65.12. | ||
:::: <span style="color:#8B008B"> '''Caution:''' KCN is photosensitive and hygroscopic; HCN is volatile and very toxic! Prepare the solution fresh everyday!</span> | |||
::::# Weigh 13 mg of KCN. ย | |||
::::# Weigh | ::::# Dissolve in 0.2 ml H<sub>2</sub>O. | ||
::::# | ::::# The pH of the solution may be very alkaline - adjust to physiological pH with HCl. | ||
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Revision as of 12:07, 22 January 2021
Description
Cyanide (usually added as KCN) is a competitive inhibitor of cytochcrome c oxidase (CIV). Inhibition is reversed by pyruvate and high oxygen levels.
Abbreviation: KCN
Reference: MiPNet09.12
MitoPedia topics: Inhibitor
Application in HRR
Inhibitor titrations, CIV-assay, alternative oxidase and ROX
- Cyanide and determination of residual oxygen consumption (ROX).
- Complex IV assay
- alternative oxidase
- O2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume: 2 ยตl of 1 M stock, using a 10 ยตl syringe (2 ml O2k-chamber).
- Final concentration: 1 mM.
- O2k manual titrations MiPNet09.12 O2k-Titrations
Preparation of 1 M KCN stock
- Kcn(KCN): Fluka 60178, 100 g, store at R.T.; FW = 65.12.
- Caution: KCN is photosensitive and hygroscopic; HCN is volatile and very toxic! Prepare the solution fresh everyday!
- Weigh 13 mg of KCN.
- Dissolve in 0.2 ml H2O.
- The pH of the solution may be very alkaline - adjust to physiological pH with HCl.