Carnitine: Difference between revisions
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|abbr=Car | |abbr=Car | ||
|description='''Carnitine''' is an important factor for the transport of long-chain fatty acids bound to carnitine ([[carnitine acyltransferase]]) into the mitochondrial matrix for subsequent ฮฒ-oxidation. There are two enantiomers: D- and L-carnitine. Only the L-isomer is physiologically active. | |description='''Carnitine''' is an important factor for the transport of long-chain fatty acids bound to carnitine ([[carnitine acyltransferase]]) into the mitochondrial matrix for subsequent ฮฒ-oxidation. There are two enantiomers: D- and L-carnitine. Only the L-isomer is physiologically active. | ||
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{{ | __TOC__ | ||
== Application in [[HRR]] == | |||
| | {{Chemical_description | ||
}} | |abbr=Car | ||
|trivial name=Carnitine | |||
|complete name=L-Carnitine inner salt | |||
|chem formula=C<sub>7</sub>H<sub>15</sub>NO<sub>3</sub> | |||
|molar mass=161.2 | |||
|vendor=Sigma-Aldrich | |||
|product number=C0158 | |||
|store at=RT | |||
|sensitivity= | |||
|cas=541-15-1 | |||
|h statements= | |||
|h info= | |||
}}<!--::::* Car:ย '''L-carnitine:''' Sigma Aldrich; C0158; M = 161.2 gยทmol<sup>-1</sup>--> | |||
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:::: '''Preparation of 250 mM stock solution''' (dissolved in H<sub>2</sub>O) | |||
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::::# Weigh 100 mg of Carnitine. | |||
::::# Add 2.48 mL H<sub>2</sub>O. | |||
::::# Divide into 100 ยตL portions. | |||
::::# Store frozen at -20ยฐC. | |||
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:::ยป '''O2k manual titrations'''ย [[MiPNet09.12 O2k-Titrations]] | |||
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::::* Titration volume ('''2-mL O2k-chamber'''): 4 ยตL using a 10 ยตL Hamilton syringe. | |||
::::* Titration volume ('''0.5-mL O2k-chamber'''): 1 ยตL using a 10 ยตL Hamilton syringe | |||
::::* Final concentration: 0.5 mM. | |||
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== Feeding carnitine == | |||
::::* [[Liu_2002_Proc_Natl_Acad_Sci_U_S_A]] | |||
::::* [[La_Guardia_2013_Front_Physiol]] | |||
::::* [[Morand_2014_Eur_J_Nutr]] | |||
::::* [[Cahova_2014_Appl_Physiol_Nutr_Metab]] | |||
::::* [[Wessels_2015_Am_J_Physiol]] | |||
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{{MitoPedia topics | {{MitoPedia topics | ||
|mitopedia topic=Substrate and metabolite | |mitopedia topic=Substrate and metabolite | ||
}} | }} | ||
= | {{Labeling | ||
|additional=MitoPedia:FAT4BRAIN | |||
}} |
Latest revision as of 17:39, 18 January 2022
Description
Carnitine is an important factor for the transport of long-chain fatty acids bound to carnitine (carnitine acyltransferase) into the mitochondrial matrix for subsequent ฮฒ-oxidation. There are two enantiomers: D- and L-carnitine. Only the L-isomer is physiologically active.
Abbreviation: Car
Application in HRR
- Car: Carnitine (L-Carnitine inner salt; C7H15NO3), Sigma-Aldrich: C0158, store at RT, CAS: 541-15-1, M = 161.2 gยทmol-1
- Preparation of 250 mM stock solution (dissolved in H2O)
- Weigh 100 mg of Carnitine.
- Add 2.48 mL H2O.
- Divide into 100 ยตL portions.
- Store frozen at -20ยฐC.
- ยป O2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume (2-mL O2k-chamber): 4 ยตL using a 10 ยตL Hamilton syringe.
- Titration volume (0.5-mL O2k-chamber): 1 ยตL using a 10 ยตL Hamilton syringe
- Final concentration: 0.5 mM.
Feeding carnitine
MitoPedia topics:
Substrate and metabolite
Labels:
MitoPedia:FAT4BRAIN