Azide: Difference between revisions

Revision as of 12:08, 28 October 2020

high-resolution terminology - matching measurements at high-resolution

Azide

Sodium azide is an inhbitor of cytochrome c oxidase (COX, CcO).

Abbreviation: Azd

Reference: MiPNet03.02

Azd: Azide (Sodium azide, NaN₃); Sigma S2002, SDS 25 g, store commercial stock at RT; FW = 65.01.

Caution: Very toxic!

Preparation of 4 M storage and stock solution (dissolved in H₂O) for 2-mL O2k-chamber:

»O2k manual titrations MiPNet09.12 O2k-Titrations

Titration volume (2-mL O2k-chamber): ≥50 µL using a 100 µL Hamilton syringe.
Final concentration: ≥100 mM.

Preparation of 2 M stock solution (dissolved in H₂O) for 0.5-mL O2k-chamber:

»O2k manual titrations MiPNet09.12 O2k-Titrations

Titration volume (0.5-mL O2k-chamber): ≥25 µL using a 100 µL Hamilton syringe.
Final concentration: ≥100 mM.

MitoPedia topics: Inhibitor

@@ Line 14: / Line 14: @@
-::::'''Preparation of 4 M storage and stock solution''' (dissolved in H<sub>2</sub>O):
+::::'''Preparation of 4 M storage and stock solution''' (dissolved in H<sub>2</sub>O)  for '''2-mL O2k-chamber''':
 ::::# Weigh 260 mg of sodium azide.
@@ Line 21: / Line 21: @@
 ::::# Store at -20 °C.
+:::»'''O2k manual titrations'''  [[MiPNet09.12 O2k-Titrations]]
+::::* Titration volume ('''2-mL O2k-chamber'''): ≥50 µL using a 100 µL Hamilton syringe.
+::::* Final concentration: ≥100 mM.
+::::'''Preparation of 2 M stock solution''' (dissolved in H<sub>2</sub>O)  for '''0.5-mL O2k-chamber''':
+::::# Take 400 µL of the 4 M stock solution
+::::# Add 400 µL H<sub>2</sub>O
 :::»'''O2k manual titrations'''  [[MiPNet09.12 O2k-Titrations]]
-::::* Titration volume: ≥50 µL using a 100 µL syringe (2 mL O2k-Chamber).
+::::* Titration volume ('''0.5-mL O2k-chamber'''): ≥25 µL using a 100 µL Hamilton syringe.
 ::::* Final concentration: ≥100 mM.