Ap5A: Difference between revisions

Latest revision as of 10:25, 21 June 2021

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Ap5A

Description

P1,P5-Di(adenosine-5')pentaphosphate (Ap5A) is an inhibitor of adenylate kinase (ADK), the enzyme which rephosphorylates AMP to ADP, consuming ATP (ATP + AMP ↔ 2 ADP).

Abbreviation: Ap5A

Application in HRR

Ap5A: Ap5A (P1,P5-Di(adenosine-5′) pentaphosphate pentasodium salt; C₂0H₂₉N₁₀O₂₂P₅ · 5 Na), Sigma-Aldrich: D4022, store at -20 °C, CAS: 4097-04-5, M = 1026.28 g·mol^-1

Preparation of 100 mM stock solution (dissolved in H₂O)

Dissolve content of Sigma 2 mg glass vial in 19.49 µL H₂O
Store at -20 °C.

» O2k manual titrations MiPNet09.12 O2k-Titrations

Titration volume (2-mL O2k-chamber): 1 µL using a 10 µL Hamilton syringe.
Final concentration: 50 µM.

References

MitoPedia topics: Inhibitor

@@ Line 3: / Line 3: @@
 |description='''P1,P5-Di(adenosine-5')pentaphosphate (Ap5A)''' is an inhibitor of [[adenylate kinase]] (ADK), the enzyme which rephosphorylates AMP to ADP, consuming ATP (ATP + AMP ↔ 2 ADP).
 }}
+__TOC__
+== Application in [[HRR]] ==
+{{Chemical_description
+|abbr=Ap5A
+|trivial name=Ap5A
+|complete name=P1,P5-Di(adenosine-5′) pentaphosphate pentasodium salt
+|chem formula=C<sub>2</sub>0H<sub>29</sub>N<sub>10</sub>O<sub>22</sub>P<sub>5</sub> · 5 Na
+|molar mass=1026.28
+|vendor=Sigma-Aldrich
+|product number=D4022
+|store at=-20 °C
+|sensitivity=
+|cas=4097-04-5
+|h statements=
+|h info=
+}}<!--:::: '''Ap5A: P1,P5-Di(adenosine-5′) pentaphosphate pentasodium salt''' Sigma D4022, store at -20 °C; molecular weight: 1026.28
+::::  <span style="color:#8B008B"> '''Caution:''' Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.</span>-->
+:::: '''Preparation of 100 mM stock solution''' (dissolved in H<sub>2</sub>O)
+::::# Dissolve content of Sigma 2 mg glass vial in 19.49 µL H<sub>2</sub>O
+::::# Store at -20 °C.
+:::» '''O2k manual titrations'''  [[MiPNet09.12 O2k-Titrations]]
+::::* Titration volume ('''2-mL O2k-chamber'''): 1 µL using a 10 µL Hamilton syringe.
+::::* Final concentration: 50 µM.
+== References ==
+::::* [https://www.ncbi.nlm.nih.gov/pubmed/6252207 Kurebayashi ''et al.'', J Biochem. 1980]
+::::* [https://www.ncbi.nlm.nih.gov/pubmed/24862274 Chinopoulos ''et al.'', Methods Enzymol. 2014]
 {{MitoPedia concepts}}
 {{MitoPedia methods}}
@@ Line 9: / Line 47: @@
 |mitopedia topic=Inhibitor
 }}
-__TOC__
-== Application in [[HRR]] ==
-:::: '''Ap5A: P1,P5-Di(adenosine-5′) pentaphosphate pentasodium salt''' Sigma D4022, store at -20 °C; molecular weight: 1026.28
-::::  <span style="color:#8B008B"> '''Caution:''' Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.</span>
-:::: '''Preparation of 100 mM stock solution''' (dissolved in H2O)
-:::::# For a 2 mg glass vial: dissolve in 19.49 µL H2O::::# Store at -20 °C.
-:::» '''O2k manual titrations'''  [[MiPNet09.12 O2k-Titrations]]
-::::* Titration volume: 1 µL using a 10 µL syringe (2 mL O2k-Chamber).
-::::* Final concentration: 50 µM.== References ==
-::::* [https://www.ncbi.nlm.nih.gov/pubmed/6252207 Kurebayashi ''et al.'', J Biochem. 1980]
-::::* [https://www.ncbi.nlm.nih.gov/pubmed/24862274 Chinopoulos ''et al.'', Methods Enzymol. 2014]