Amplex UltraRed: Difference between revisions
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This method can be used with the [O2k-Fluorescence LED2-Module]]<ref>[[Hickey 2012 J Comp Physiol B]]</ref>. In addition, the Oxygraph-2k has been coupled to full fluorescence spectrophotometers for this purpose, with a light guide inserted through the [[Stopper\black PEEK\conical Shaft\central+side 2.5 mm Port|black PEEK stopper]]<ref> [[Anderson 2011 Am J Physiol Heart Circ Physiol]])</ref>. | This method can be used with the [O2k-Fluorescence LED2-Module]]<ref>[[Hickey 2012 J Comp Physiol B]]</ref>. In addition, the Oxygraph-2k has been coupled to full fluorescence spectrophotometers for this purpose, with a light guide inserted through the [[Stopper\black PEEK\conical Shaft\central+side 2.5 mm Port|black PEEK stopper]]<ref> [[Anderson 2011 Am J Physiol Heart Circ Physiol]])</ref>. | ||
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Revision as of 11:49, 4 May 2012
- high-resolution terminology - matching measurements at high-resolution
Amplex UltraRed
Description
Amplex red (AmR) belongs to the extrinsic fluorophores and makes use of the fact that ROS generate hydrogen peroxide (H2O2) and it is the H2O2 level that Amplex red is used to measure. In the presence of horseradish peroxidase, Amplex red reacts with H2O2 to produce the red fluorescent compound resorufin (excitation wavelength 563 nm, emission 587 nm). The emitted fluorescence intensity is directly proportional to the H2O2 concentration.
Abbreviation: AmR
Reference: Mishin 2010 Free Radical Biol Med, Towne 2004 Anal Biochem, Hickey 2012 J Comp Physiol B
MitoPedia methods:
Fluorometry
This method can be used with the [O2k-Fluorescence LED2-Module]][1]. In addition, the Oxygraph-2k has been coupled to full fluorescence spectrophotometers for this purpose, with a light guide inserted through the black PEEK stopper[2].
References
