Air calibration for high O2 experiments
From Bioblast
Question: For a experiment with with high (above atmospheric levels) O2 concentrations I have to change the gain and therefore to perform a new "air calibration". How to do this?
Answer: The air calibration has always to be done at atmospheric saturation (a point of known O2 conc.), but at the correct Gain
- set the gain to 1
- perform air calibration at atmospheric saturation, open chamber, near zero flux at open chamber, as usually
- close the chamber
- wait until you observe a stable flux at closed chamber near to air saturation of 2 to 4 pmol/s ml (quality control for biological contamination)
- increase the O2 concentration either with a gas phase method or (in MiR06) with H2O2 injections.
See also:
Air calibration, Raw_Signal, Gain (O2_channel)
