Additive effect of convergent electron flow: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
Electron flow converges at the | Electron flow converges at the '''Q-junction''' from respiratory Complexes I and II ([[CI+II e-input]]), [[glycerophosphate dehydrogenase]] and [[electron-transferring flavoprotein]]. Convergent electron flow corresponds to the operation of the [[TCA cycle]] and mitochondrial substrate supply in vivo. | ||
==Additive effect== | ==Additive effect== | ||
Convergent electron flow simultaneously through CI+II into the [[Q-junction]] supports higher [[OXPHOS]] and [[ETS]] capacities than separate electron flow through either CI or CII. Physiological substrate combinations supporting convergent CI+II e-input are required for reconstitution of intracellular [[TCA cycle]] function. The convergent CI+II effect may be completely or partially additive, suggesting that conventional bioenergetic protocols with [[mt-preparations]] have underestimated cellular OXPHOS capacities. | Convergent electron flow simultaneously through CI+II into the [[Q-junction]] supports higher [[OXPHOS]] and [[ETS]] capacities than separate electron flow through either CI or CII. Physiological substrate combinations supporting convergent CI+II e-input are required for reconstitution of intracellular [[TCA cycle]] function. The convergent CI+II effect may be completely or partially additive, suggesting that conventional bioenergetic protocols with [[mt-preparations]] have underestimated cellular OXPHOS capacities. | ||
Revision as of 15:20, 15 August 2010
Electron flow converges at the Q-junction from respiratory Complexes I and II (CI+II e-input), glycerophosphate dehydrogenase and electron-transferring flavoprotein. Convergent electron flow corresponds to the operation of the TCA cycle and mitochondrial substrate supply in vivo.
Additive effect
Convergent electron flow simultaneously through CI+II into the Q-junction supports higher OXPHOS and ETS capacities than separate electron flow through either CI or CII. Physiological substrate combinations supporting convergent CI+II e-input are required for reconstitution of intracellular TCA cycle function. The convergent CI+II effect may be completely or partially additive, suggesting that conventional bioenergetic protocols with mt-preparations have underestimated cellular OXPHOS capacities.
References
- Gnaiger E (2009) Mitochondrial pathways through Complexes I+II: Convergent electron transport at the Q-junction and additive effect of substrate combinations. Mitochondr. Physiol. Network 12.12: 1-20.
- Gnaiger E (2009) Capacity of oxidative phosphorylation in human skeletal muscle. New perspectives of mitochondrial physiology. Int. J. Biochem. Cell Biol. 41: 1837–1845.
