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...ransfer from beta-oxidation and TCA cycle and impact of OXPHOS coupling on NADH and coenzyme Q redox states. ...o analyze the relationships between the N- and Q-redox states and electron transfer rates. <br>

...e the reduction level of NADP and NAD, which, in turn, affects respiratory electron flow through succinate dehydrogenase.

...aila VR, Wikström M, Hummer G (2014) Electrostatics, hydration, and proton transfer dynamics in the membrane domain of respiratory Complex I. Proc Natl Acad Sc ...lecules establishing enhanced lateral connectivity and pathways for proton transfer between conserved ionizable residues along the center of the membrane domai

...and glycerophosphate-pathways to the Q-junction regulate reversed electron transfer to Complex I and H₂O₂ production. ...pressure generated by the linear S- and Gp-pathways or the convergent SGp-pathway on the Q-junction drives RET into CI and thus regulates H₂O<sub>

...are designed to measure simultaneously O₂ flux and the Q-redox state in isolated mitochondria and permeabilized cells. ...t D072]] simultaneous determination of O₂ flux and the Q-redox state in isolated mitochondria and cells permeabilized previously to addition to

...support NADH-linked respiration. Oligomycin (Omy) is used to induce a LEAK state of respiration via the inhibition of the ATP synthase. Since higher concent :::* Comparison of GM- with PM-capacity yields important information on N-pathway respiratory control upstream of CI.

...r W (2016) Adapting high-resolution respirometry to glucose-limited steady state mycelium of the filamentous fungus ''Penicillium ochrochloron'': method dev ...y does not depend on a complete saturation or inhibition of the cytochrome pathway.

...f the ATP synthase. Since higher concentrations of Omy can decrease the ET state induced upon addition of uncoupler, the required concentration of Omy has t :::* Comparison of GM- with PM-capacity yields important information on N-pathway respiratory control upstream of CI.

|abbr=''ET-pathway state'' ...[[Categories of SUIT protocols]] are defined according to mitochondrial ET-pathway states.

...ain components. These interactions would regulate mitochondrial CoQ steady-state levels and function. This article is part of a Special Issue entitled 'EBEC

|abbr=NADH ...dition of NADH may indicate an alteration of the mtIM integrity. Cytosolic NADH is effectively made available for mitochondrial respiration through the [[m

...-[[ET capacity| ''E'']]) with NADH-linked substrates ([[PM-pathway control state|PM]] and PGM)''' ...may be relevant in the presence of [[glutamate-anaplerotic pathway control state]]. SUIT-012 can be extended with the CIV assay module.

...den Bergen CW, Wagner AM, Krab K, Moore AL (1994) The relationship between electron flux and the redox poise of the quinone pool in plant mitochondria. Interpl ...oplasmic NADH ''in vivo'' uses the cyanide-resistant pathway more than the pathway involving the oxidation of succinate. A model is used to predict the kineti

...f the ATP synthase. Since higher concentrations of Omy can decrease the ET state induced upon addition of uncoupler, the required concentration of Omy has t :::* Comparison of GM- with PM-capacity yields important information on N-pathway respiratory control upstream of CI.

...Rich PR, Gnaiger E (2021) Coupling and pathway control of coenzyme Q redox state and respiration in isolated mitochondria. https://doi.org/10.26124/mitofit: ...omlodi_2021_MitoFit_Q.pdf Coupling and pathway control of coenzyme Q redox state and respiration in isolated mitochondria]

:::* Comparison of GM- with PM-capacity yields important information on N-pathway respiratory control upstream of CI. ...tate| S]] and [[NADH Electron transfer-pathway state| N-]] pathway control state.

...rdoso LHD, Donnelly C, Komlódi T, Gnaiger E (2022) Characterizing electron transfer through the mitochondrial Q-junction from fatty acid oxidation and TCA cycl ...posite directions. Therefore, our aim was to characterize the influence of pathway and coupling control on these two bioenergetic parameters.

|info='''A''' Linear coupling control with NADH-linked substrates for isolated mitochondria, tissue homogenate and permeabi ...he involvement of the [[complex II]] ([[Succinate pathway control state| S-pathway]]). It can be useful to understand the contribution and the activity of the

...nitoring of respiration together with NAD(P)H autofluorescence and Q-redox state [1] provides unique analytical and diagnostic power in the study of mitocho ...Rich PR, Gnaiger E (2021) Coupling and pathway control of coenzyme Q redox state and respiration in isolated mitochondria. https://doi.org/10.26124/bec:2021

...K state. Pyruvate (P) supports [[NADH Electron transfer-pathway state|NADH-pathway (N)]], and usually in the presence of ADP it does not increase further the :::: 1. the S- and NS-pathway-linked respiration and H₂O₂ flux.