Template:Chemical SUIT-006 NADH mt D084: Difference between revisions
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| 4U | | 4U | ||
| {{Template:SF6847}} | | {{Template:SF6847}} | ||
| | | We do not recommend the use of any other uncoupler, like {{Template:CCCP}}, due to background fluorescence. | ||
|- | |- | ||
| 5Anox | | 5Anox | ||
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| 6Myx | | 6Myx | ||
| {{Template:Myxothiazol}} | | {{Template:Myxothiazol}} | ||
| | | We do not recommend the use of any other inhibitor of complex III, like {{Template:Antimycin A}}, due to background fluorescence. | ||
|- | |- | ||
| 7Reox | | 7Reox |
Revision as of 13:00, 14 December 2023
Step | Chemical(s) and link(s) | Comments |
---|---|---|
1PGM | Pyruvate (P), Glutamate (G), and Malate (M) | |
2D | ADP (D) | |
(3Omy) | Oligomycin (Omy) | This step can be skipped. |
4U | SF6847 | We do not recommend the use of any other uncoupler, like Carbonyl cyanide m-chlorophenyl hydrazone, CCCP (U), due to background fluorescence. |
5Anox | The O2 concentration in the O2k-chamber can be decreased by N2 or H2 injection to reach faster anoxia, see: Setting the oxygen concentration. | |
6Myx | Myxothiazol | We do not recommend the use of any other inhibitor of complex III, like Antimycin A (Ama), due to background fluorescence. |
7Reox |