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| ::: [[Cardoso LHD]] has been accessed more than | | ::: [[Cardoso LHD]] has been accessed more than |
| ::::* 5,000 times (2019-12-16) | | ::::* 5,000 times (2019-12-16) |
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| ::::[[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]] Do you know how to evaluate '''fatty acid oxidation''' avoiding to overestimate it?'''
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| :::::::- Did you know that '''malate alone might support mt-respiration''', e.g. if the [[Malic enzyme| mt-malic enzyme]] is present in [[Mitochondrial preparations| mitochondrial preparations]]? Learn more about - »'''[[Malate anaplerotic pathway control state|malate anaplerotic pathway control state]]'''«
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| :::::::- '''DatLab 7.4''' provides a step-by-step guide through the '''SUIT-027''' to evaluate the malate anaplerotic pathway - »'''[[SUIT-027 |SUIT-027]]'''«
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| :::::::- [[Fatty acid oxidation pathway control state|F-pathway]] can be overestimated due to anaplerotic reactions. '''SUIT-002''' is specially designed to give information on '''F-pathway in OXPHOS state avoiding FAO overestimation''' in the presence of [[Anaplerosis|anaplerotic pathways]] - »'''[[SUIT-002 |SUIT-002]]'''«
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| ::::[[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]] How can I measure '''ATP production''' in my '''cultured cells'''?
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| :::::::- Measuring respiration of living cells will provide you important parameters, but calling it ATP production is not correct - »'''[[Magnesium_Green#Is_it_possible_to_measure_ATP_production_in_the_protocol_SUIT-003_O2_ce_D009.3F|SUIT protocols to measure ATP production]]'''«
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| :::::::- Learn more about how to measure ATP production in mitochondrial preparations with Magnesium Green - »'''[[Magnesium_Green|Magnesium Green]]'''«
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