MiPNet25.03 IOC144 Innsbruck AT: Difference between revisions

From Bioblast
No edit summary
Line 3: Line 3:
{{Publication
{{Publication
|title=[[Image:OROBOROS-solo.png|right|60px|link=OROBOROS INSTRUMENTS]]
|title=[[Image:OROBOROS-solo.png|right|60px|link=OROBOROS INSTRUMENTS]]
'''Innsbruck AT''', 2020 Jan 20. MitoFit Coaching Days - Basic '''IOC'''.
'''Innsbruck AT''', 2020 Jan 20-22. MitoFit Coaching Days - Basic '''IOC'''.
|authors=Oroboros
|authors=Oroboros
|year=2020-01-20
|year=2020-01-20
Line 12: Line 12:
}}
}}
{{Labeling
{{Labeling
|additional=ORO, IOC, MitoFit, TRANSMIT, MitoGlobal, Next, 2020,
|additional=ORO, IOC, MitoFit, MitoGlobal, Next, 2020,
}}
}}


Line 18: Line 18:


== General information ==
== General information ==
[[File:TRANSMIT Acronym.jpg|100px|right|TRANSMIT]]
[[File:REMIX.png|100px|right|REMIX]]
:::: '''MitoFit Coaching Days - Basic''' provide a fundamental introduction to high-resolution respirometry with the Oroboros O2k. Hands-on sessions range from OroboPOS technical service to respirometry experiments with substrate-uncoupler-inhibitor (SUIT) protocols.
:::: '''MitoFit Coaching Days - Basic''' provide a fundamental introduction to high-resolution respirometry with the Oroboros O2k. Hands-on sessions range from OroboPOS technical service to respirometry experiments with substrate-uncoupler-inhibitor (SUIT) protocols.
:::: Early stage researchers (ESRΒ΄s) during their Marie SkΕ‚odowska-Curie project - [[TRANSMIT]] or [[REMIX]] will be trained at the O2k-Workshop on high-resolution respirometry:
:::::Β» http://remix-itn.eu/
:::::Β» https://www.transmit-project.eu
<br/>
::::Β» Information on travel and venue: '''[[IOC Innsbruck]]'''.


:::: Please bring your labcoat with you. Thank you!
:::: Please bring your labcoat with you. Thank you!


== Feedback ==
::::11 feedback forms returned
::::* ''Instructors - so patient! So kind! Really good at explaining things over and over again.''
::::* ''Thank you to all involved including other participants who were also kind and keen to learn and share. ''
::::* ''Great company and team!''


{| class="wikitable"
== Venue ==
|-
! Question
! Rating (average)
|-


|In how far were your interests covered in the workshop? Please rate from 1 (not covered) to 5 (all interests covered).
::::Β» Information on travel and venue: '''[[IOC Innsbruck]]'''.
|4.27
::::Β» Hotel suggestion: [http://www.weisseskreuz.at/de/index.php Hotel Weisses Kreuz]
|-
|Balance between practical β€œhands-on” work and β€œtheoretical” explanations: Please rate from 1 (too much β€œhands-on”) to 5 (too much theory).
|3
|-
|Presentation of lectures: Please rate from 1 (not understandable) to 5 (very understandable). a. in terms of content:
|4.18
|-
|Presentation of lectures: Please rate from 1 (not understandable) to 5 (very understandable). b. in terms of language:
|4.36
|-
|Level of tutoring during the β€œhands-on” work: Please rate from 1 (not enough tutoring, I felt left alone) to 5 (too much tutoring, I would have liked to work more independently).
|3.14
|-
|Quality of tutoring: Please rate from 1 (poor) to 5 (excellent).
|4.82
|-
|Intensity of the O2k-Course: Please rate from 1 (I would have liked to do and hear more) to 5 (I felt overwhelmed).
|2.77
|-
|Your overall rating of the course? Please rate from 1 (poor) to 5 (excellent). Β 
|4.32
|}


== Registration ==
== Registration ==
:::: closed
in progress


== Tutors, lecturers, and managers ==
== Tutors, lecturers, and managers ==
<br/>
in progress
<gallery mode=default perrow=6 widths="140px" heights="150px">
File:PassruggerM.JPG|'''[[Passrugger M |Manuela Passrugger]]''', BSc., Oroboros Instruments - Biomedical Assistant
File:Di MarcelloM.jpg|'''[[Di Marcello M|Marco di Marcello]]''', BSc., Medical University of Innsbruck, MitoFit project - Assistant
File:Bastos Sant'Anna Silva AC.jpg|'''[[Bastos Sant'Anna Silva AC|Ana Carolina Bastos Sant'Anna Silva]]''', MSc., Oroboros Instruments - PhD. student TRANSMIT
File:GarciaL.JPG|'''[[Garcia e Souza Luiz Felipe]]''', MSc., Leopold-Franzens-University - PhD. student
File:DoerrierC.JPG|'''[[Doerrier C|Carolina Doerrier]]''', PhD., Oroboros Instruments - Chief scientific officer
File:Gnaiger Erich.jpg|'''[[Gnaiger E|Erich Gnaiger]]''', PhD., Oroboros Instruments - CEO
</gallery>
Β 


== Programme ==
== Programme ==
::::{| class='wikitable'
::::will be uploaded
|-
|style="width: 10em"|'''Monday 20th'''
|style="width: 60em"|'''Topic'''
|style="width: 15em"|'''Room'''
|-
|08:15
|Welcome - Get-together: Introduction of participants and their research interests
|ORO-MitoFit Lab
|-
|08:30
|OroboPOS service and O2k instrumental setup - overview with videoclips
|ORO-MitoFit Lab
|-
|09:30
|'''Hands-on''': OroboPOS service and O2k instrumental setup
|ORO-MitoFit Lab
|-
|10:10
|'''Hands-on''': DatLab 7
|ORO-MitoFit Lab
|-
|10:30
|''Coffee''
|
|-
|11:00
|Instrumental quality control 1: oxygen calibration
|ORO-MitoFit Lab
|-
|11:30
|'''Hands-on''': Instrumental quality control 1: oxygen calibration
Β 
DL-Protocol: O2k-cleaning before use
Β 
DL-Protocol: O2 calibration air
|ORO-MitoFit Lab
|-
|12:00
|''Lunch break''
|
|-
|13:00
|'''Hands-on''':Instrumental quality control 1: oxygen calibration (continuation)
DL-Protocol: O2 calibration air
|ORO-MitoFit Lab
|-
|13:30
|Instrumental quality control 2: Instrumental O2 background – overview with videoclips
|ORO-MitoFit Lab
|-
|14:00
|'''Hands-on''':Instrumental quality control 2: Instrumental O2 background
DL-Protocol: Instrumental O2 background TiP2k
|ORO-MitoFit Lab
|-
|15:00
|''Coffee''
|
|-
|15:30-18:00
|'''Hands-on''': Instrumental quality control 1 and 2. DatLab analysis.
|ORO-MitoFit Lab
|-
|}
Β 
::::{| class='wikitable'
|-
|style="width: 10em"|'''Tuesday 21th'''
|style="width: 60em"|'''Topic'''
|style="width: 15em"|'''Room'''
|-
|09:00
|Hands-on: Instrumental quality control 1: oxygen calibration
Β 
DL-Protocol: O2k-cleaning before use
Β 
DL-Protocol: O2 calibration air
|ORO-MitoFit Lab
|-
|09:15
|Application of the O2k Series HΒ 
|ORO-MitoFit Lab
|-
|09:30
|Introduction to substrate-uncoupler-inhibitor titration (SUIT) protocols – fundamental principles. SUIT reference protocol: RP1&RP2
|ORO-MitoFit Lab
|-
|10:00
|''Coffee''
|
|-
|10:30
|'''Hands-on''': O2k-Demo experiment: Respiration of permeabilized cells: measurement of oxygen consumption with RP1 and RP2.
Β 
DL-Protocol: SUIT-1_O2_pce_D03.DLP
Β 
DL-Protocol: SUIT-2 O2 pce D07.DLP
Β 
|ORO-MitoFit Lab
|-
|11:45
|'''Hands-on''': O2k-cleaning after use
Β 
DL-Protocol: O2k-cleaning after use
|ORO-MitoFit Lab
|-
|12:00
|''Lunch break''
|
|-
|13:00
|'''Hands-on''': O2k-cleaning after use
Β 
DL-Protocol: O2k-cleaning after use
|ORO-MitoFit Lab
|-
|13:30
|Introduction to DatLab analysis
|ORO-MitoFit Lab
|-
|15:00
|''Coffee''
|
|-
|15:30-18:00
|Individual DatLab analysis + summary
|ORO-MitoFit Lab
|-
|}
Β 
::::{| class='wikitable'
|-
|style="width: 10em"|'''Wednesday 22th'''
|style="width: 60em"|'''Topic'''
|style="width: 15em"|'''Room'''
|-
|09:00
|'''MitoFit proficiency test:'''
Β 
'''Hands-on''': Instrumental quality control 1: oxygen calibration
Β 
DL-Protocol: O2k-cleaning before use
Β 
DL-Protocol: O2 calibration air
Β 
|ORO-MitoFit Lab
|-
|10:00
|''Coffee''
|
|-
|10:30
|'''MitoFit proficiency test:'''
Β 
'''Hands-on''': O2k-Demo experiment: Respiration of permeabilized cells: measurement of oxygen consumption with RP1 and RP2.
Β 
DL-Protocol: SUIT-1_O2_pce_D03.DLP
Β 
DL-Protocol: SUIT-2 O2 pce D07.DLP
Β 
|ORO-MitoFit Lab
|-
|11:45
|'''Hands-on''': O2k-cleaning after use
Β 
DL-Protocol: O2k-cleaning after use
|ORO-MitoFit Lab
|-
|12:00
|Lunch break
|
|-
|13:00
|'''Hands-on''': O2k-cleaning after use
Β 
DL-Protocol: O2k-cleaning after use
|ORO-MitoFit Lab
|-
|13:15
|DatLab analysis
|ORO-MitoFit Lab
|-
|14:00
|DatLab analysis summary
|ORO-MitoFit Lab
|-
|15:00
|''Coffee''
|
|-
|15:30
|The Bioblast wiki, O2k-Network and MITOEAGLE
|MiP''Art''
|-
|16:15
|Feedback & conclusions
|MiP''Art''
|-
|17:00
|Farewell activity
|MiP''Art''
|-
|}


== Participants ==
== Participants ==
{| class="wikitable"
|-
!
! Participant
! Institution
|-
| [[File:Profile-icon-9.png|70px|Participant]]
| [[Barlow Jonathan]]
| '''[[UK Birmingham Lai YC]]''', University of Birmingham, UK
|-
| [[File:Chakrabarti L.jpg|70px|Participant]]
| [[Chakrabarti Lisa]]
| '''UK Nottingham Chakrabarti L''', University of Nottingham, UK
|-
| [[File:GnaigerC.JPG|70px|Participant]]
| [[Gnaiger Carolina]]
| '''[[AT Innsbruck Oroboros]]''', Innsbruck, AT
|-
| [[File:Profile-icon-9.png|70px|Participant]]
| [[Ho DH|Ho Dieu Hien]]
| '''[[DE Cologne Trifunovic A]]''', Institute of Mitocondiral Disease and Ageing, Cologne, DE
|-
| [[File:Profile-icon-9.png|70px|Participant]]
| [[Jedlicka Jan]]
| '''[[CZ Pilsen Kuncova J]]''', Charles University, Plzne, CZ
|-
| [[File:Profile-icon-9.png|70px|Participant]]
| [[Kapferer Werner]]
| '''[[AT Innsbruck Jansen-Duerr P]]''', University of Innsbruck, AT
|-
| [[File:KranewitterS.JPG|70px|Sabine]]
| [[Kranewitter S|Kranewitter Sabine]]
| '''[[AT Innsbruck Oroboros]]''', Innsbruck, AT
|-
| [[File:Profile-icon-9.png|70px|Participant]]
| [[Maciej Sarah]]
| '''[[DE Cologne Trifunovic A]]''', Institute of Mitocondiral Disease and Ageing, Cologne, DE
|-
| [[File:Profile-icon-9.png|70px|Participant]]
| [[Maseko Tumisang Edward]]
| '''[[CZ Hradec Kralove Cervinkova Z]]''', Charles University, Hradec Kralove, CZ
|-
| [[File:Profile-icon-9.png|70px|Participant]]
| [[Oliveira Ben]]
| '''UK Leicester Watson E''', Kings College Hospital, London, UK
|-
| [[File:Profile-icon-9.png|70px|Participant]]
| [[Saharnaz Sarlak]]
| '''[[FR Bordeaux Rossignol R]]''', University of Bordeaux, Bordeaux, FR
|-
| [[File:Profile-icon-9.png|70px|Participant]]
| [[Thapa Maheshwor]]
| '''[[AT Salzburg Sperl W]]''', Biocrates Life Sciences AG, Innsbruck, AT
|-
| [[File:Profile-icon-9.png|70px|Participant]]
| [[Ton Riccardo]]
| '''AU Sydney Griffith SC''', Macquarie University, Sydney, AU
|-
| [[File:Profile-icon-9.png|70px|Participant]]
| [[Zdrazilova Lucie]]
| '''[[CZ Prague Zeman J]]''', Charles University, Prague, CZ
|-
|}
== Impressions of the IOC137 ==
<gallery mode=default perrow=4 widths="350px" heights="350px">
File:IOC137 1.JPG|Group picture of IOC137 in front of the Oroboros Office
File:DSC 0067.JPG|Group picture of IOC137 participants and tutors
File:IOC137 2.JPG|[[Passrugger M|Manuela Passrugger]] and [[Bastos Sant'Anna Silva AC|Ana Carolina Bastos Sant'Anna Silva]] assisting while mounting membranes
File:IOC137 10.JPG|Snapshot of some IOC137 participants during [[Di Marcello M|Marco di Marcello]]'s presentation
File:IOC136 4.JPG|[[Ho Dieu Hien]], [[Oliveira Ben]], [[Ton Riccardo]] and [[Maciej Sarah]] working with the O2k
File:IOC137 4.JPG|Participants in action
File:IOC137 11.JPG|[[Kapferer Werner]] and [[Barlow Jonathan]] running the experiment with [[Garcia-Souza LF |Luiz Felipe Garcia e Souza]]'s support
File:IOC137 5.JPG|Snapshot of [[Maseko Tumisang Edward]]
File:IOC137 7.JPG|[[Saharnaz Sarlak]] studying the material hand-outs
File:IOC137 8.JPG|[[Passrugger M|Manuela Passrugger]] assisting the Oroboros-Team members [[Gnaiger C|Gnaiger Carolina]] and [[Kranewitter S|Sabine Kranewitter]] during the experiment
File:IOC137 6.JPG|[[Doerrier C|Carolina Doerrier]] and [[Ton Riccardo]] after the successful experiment
</gallery>




Revision as of 10:14, 2 August 2019

                

MiPNet25.03 IOC144 Innsbruck AT


Publications in the MiPMap
OROBOROS-solo.png

Innsbruck AT, 2020 Jan 20-22. MitoFit Coaching Days - Basic IOC.


Oroboros (2020-01-20) Mitochondr Physiol Network

Abstract:

O2k-Workshops.png

MitoFit Coaching Days - Basic. Innsbruck, Austria; 2020 Jan 20-22.


β€’ O2k-Network Lab: AT_Innsbruck_Oroboros, AT_Innsbruck_MitoFit


Labels:






ORO, IOC, MitoFit, MitoGlobal, Next, 2020 


General information

MitoFit Coaching Days - Basic provide a fundamental introduction to high-resolution respirometry with the Oroboros O2k. Hands-on sessions range from OroboPOS technical service to respirometry experiments with substrate-uncoupler-inhibitor (SUIT) protocols.
Please bring your labcoat with you. Thank you!


Venue

Β» Information on travel and venue: IOC Innsbruck.
Β» Hotel suggestion: Hotel Weisses Kreuz

Registration

in progress

Tutors, lecturers, and managers

in progress

Programme

will be uploaded

Participants

Contact

[email protected]


Oroboros Instruments
High-resolution respirometry
SchΓΆpfstrasse 18
A-6020 Innsbruck, Austria
Tel: +43 512 566796
Fax: +43 512 566796 20
Mitochondria and cell research

Project support

Contribution to K-Regio project MitoFit, funded by the Land Tirol within the program K-Regio of Standortagentur Tirol.
Sponsors.png

IOC recommended reading

Β» IOC recommended reading
Β» MitoPedia: Respiratory states
OXPHOS ROUTINE ETS LEAK ROX


MitoGlobal
O2k-Workshops and MitoFit Coaching Days are listed as MitoGlobal Events.
Retrieved from "https://wiki.oroboros.at/index.php?title=MiPNet25.03_IOC144_Innsbruck_AT&oldid=183652"
Cookies help us deliver our services. By using our services, you agree to our use of cookies.
More information
Powered by MediaWiki
Powered by Semantic MediaWiki