Sample and medium - DatLab

MitoPedia O2k and high-resolution respirometry: O2k-Open Support

Description

DatLab 8: In the window Sample and medium, information is entered and displayed for the sample and medium. Entries can be edited any time during the experiment or during post-experiment analysis. All related results are recalculated instantaneously with the new parameters. The window can be opened whenever a file is loaded or currently recorded.

DatLab 7: Sample

Abbreviation: F3

Experiment menu

The following entries, excluding experimental code and comments, are entered separately for the left (A) and right (B) O2k-chamber:

Experimental code: Enter a code for your experiment.

Protocol: (read only) Displays the name of the DL8-Protocol selected (if applicable).

Sample type: Enter the type of sample used in the experiment.

Sample preparation: Select a predefined sample preparation or 'other' in the drop-down menu. If you start with chamber A, the field for chamber B will be filled accordingly. Chamber B can be set to a different sample preparation than chamber A by selecting another option in the corresponding drop-down menu. The following sample preparations are available:

imt for isolated mitochondria

thom for tissue homogenate

pfi for permeabilized muscle fibers

pti for permeabilized tissue

ti for tissue

pce for permeabilized cells

ce for living cells

ce to pce for living cells permeabilized during the experiment

org for intact organisms

other for none of the ones listed above

More details: Sample

Cohort, Sample code: Add information about your sample by entering any combination of digits and numbers to chamber A. Chamber B will be set accordingly but can be changed to any other value.

Sample number: Enter a sample number in chamber A. Chamber B is set accordingly but can be edited.

Subsample number: Enter a subsample number. If a number is entered to chamber A, the number plus one will be entered to chamber B.

Medium: Enter the name of the incubation medium in the O2k-chamber.

Chamber volume [mL]: The standard volumes are 2 mL, or 0.5 mL. The value is imported from the “Start recording” window. It is important to define the actual effective volume of the O2k-chamber used for further calculations of oxygen flux, H₂O₂ flux, etc. For calibration of the O2k-chamber volume see: »MiPNet28.07 NextGen-O2k Series XB manual.

Normalization Information entered in this section is necessary to normalize the recorded data for the amount or concentration of sample in the O2k-chamber (see Flux_/_Slope for further details). An overview of recorded data is displayed on the mark statistics page that is accessible via 'Marks' in the top menu bar or by pressing F2. Entries in the fields 'Type' and 'Unit' are used for the label of the specific flux (or 'flow' in case of cells), 'Amount' or 'Concentrations' are used to compute the value of the specific flux (or 'flow' in case of cells).

Set from predefined normalization A drop-down menu provides support to enter correct values for 'Type' and 'Unit'. Select 'other' to leave both fields empty.

dry mass the Type field is set to 'dry mass' and the Unit field is set to 'mg'

wet mass the Type field is set to 'wet mass' and the Unit field is set to 'mg'

mass of protein the Type field is set to 'mass of protein' and the Unit field is set to 'mg'

cell count the Type field is set to 'cell count' and the Unit field is set to 'Mx' (i.e., million cells)

other Type and Unit fields can be set manually. They can also be set manually by simply overwriting one of the predefined values.

Amount per chamber / Concentration per mL Enter the sample concentration or the sample amount in the respective field. The value in the other field will be calculated automatically by taking the volume of the O2k-chamber into account.

Comments can be given in the bottom field.

MitoPedia O2k and high-resolution respirometry: DatLab