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Dechandt 2019 Front Neurol

From Bioblast
Publications in the MiPMap
Dechandt CRP, Ferrari GD, Dos Santos JR, de Oliveira JAC, da Silva-Jr RMP, Cunha AOS, Garcia-Cairasco N, Alberici LC (2019) Energy metabolism and redox state in brains of wistar audiogenic rats, a genetic model of epilepsy. Front Neurol 10:1007.

» PMID: 31632331 Open Access

Dechandt CRP, Ferrari Gustavo Duarte, Dos Santos JR, de Oliveira JAC, da Silva-Jr RMP, Cunha AOS, Garcia-Cairasco N, Alberici Luciane Carla (2019) Front Neurol

Abstract: The Wistar Audiogenic Rat (WAR) strain is a genetic model of epilepsy, specifically brainstem-dependent tonic-clonic seizures, triggered by acute auditory stimulation. Chronic audiogenic seizures (audiogenic kindling) mimic temporal lobe epilepsy, with significant participation of the hippocampus, amygdala, and cortex. The objective of the present study was to characterize the mitochondrial energy metabolism in hippocampus and cortex of WAR and verify its relationship with seizure severity. Hippocampus of WAR naïve (no seizures) presented higher oxygen consumption in respiratory states related to the maximum capacities of phosphorylation and electron transfer system, elevated mitochondrial density, lower GSH/GSSG and catalase activity, and higher protein carbonyl and lactate contents, compared with their Wistar counterparts. Audiogenic kindling had no adding functional effect in WAR, but in Wistar, it induced the same alterations observed in the audiogenic strain. In the cortex, WAR naïve presented elevated mitochondrial density, lower GSH/GSSG and catalase activity, and higher protein carbonyl levels. Chronic acoustic stimulation in Wistar induced the same alterations in cortex and hippocampus. Mainly in the hippocampus, WAR naïve presented elevated mRNA expression of glucose, lactate and excitatory amino acids transporters, several glycolytic enzymes, lactate dehydrogenase, and Na+/K+ ATPase in neurons and in astrocytes. In vivo treatment with mitochondrial uncoupler 2,4-dinitrophenol (DNP) or N-acetylcysteine (NAC) in WAR had no effect on mitochondrial metabolism, but lowered oxidative stress. Unlike DNP, NAC downregulated all enzyme genes involved in glucose and lactate uptake, and metabolism in neurons and astrocytes. Additionally, it was able to reduce brainstem seizure severity in WAR. In conclusion, in WAR naïve animals, both cerebral cortex and hippocampus display elevated mitochondrial density and/or activity associated with oxidative damage, glucose and lactate metabolism pathways upregulation, and increased Na+/K+ ATPase mRNA expression. Only in vivo treatment with NAC was able to reduce seizure severity of kindled WARs, possibly via down regulation of glucose/lactate metabolism. Taken together, our results are a clear contribution to the field of mitochondrial metabolism associated to epileptic seizures.

Copyright © 2019 Dechandt, Ferrari, dos Santos, de Oliveira, Silva-Jr, Cunha, Garcia-Cairasco and Alberici. Keywords: 2, 4-dinitrophenol (DNP), N-acetylcysteine (NAC), Na+/K+ATPase, Audiogenic kindling, Glycolysis, Mitochondria, Reactive oxygen species, Wistar audiogenic rats (WAR) Bioblast editor: Plangger M O2k-Network Lab: BR Ribeirao Preto Alberici LC


Labels: MiParea: Respiration, nDNA;cell genetics  Pathology: Other 

Organism: Rat  Tissue;cell: Nervous system  Preparation: Permeabilized tissue 


Coupling state: LEAK, OXPHOS, ET  Pathway: N, ROX  HRR: Oxygraph-2k 

Labels, 2019-11