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COST Action CA15203 (2016-2021): MitoEAGLE
Evolution-Age-Gender-Lifestyle-Environment: mitochondrial fitness mapping
MitoEAGLE protocols, terminology, documentation
WG1
- Standard operating procedures and user requirement document: Protocols, terminology, documentation
WG1 Description
- The quality management system, scope of SOPs to be elaborated and the outline of the data repositories will be defined in a user requirement document.
Respirometric reference protocols
- A ‘library of protocols’ applied in mitochondrial respiratory physiology will be collected in a standard format, delineating the diversity of experimental approaches to assess bioenergetic function. A set of reference protocols will rigorously document (Maelstrom Research program) the state-of-the-art standards in designing, conducting, reporting, interpreting, and validating such bioenergetic tests (see also Fig. 3).
Interlaboratory proficiency test
- Inter-laboratory ring tests are a basic requirement for quality management. Such proficiency tests require a reference sample which must be homogenous, stable, representative of the diagnostic target, amenable for distribution, and economical for large-scale production. For respiratory OXPHOS analysis a reference sample of structurally and functionally intact mitochondria is not yet available to perform corresponding ring tests (in contrast to enzymatic OXPHOS ring tests). Recently, the widely applied human cell line HEK 293T was found to be potentially suitable for cryopreservation. Upon thawing the cells can be used immediately for respirometry of both intact and permeabilized cells. Thus interlaboratory proficiency testing may now be feasible as a world-wide innovation in the field of mitochondrial respiratory physiology. Participating labs may implement the test for intra- and interlaboratory validation and longitudinal performance monitoring, using a reference OXPHOS protocol (Fig. 3). The organization of the proficiency test should follow the requirements of the ISO 17043 and 13528 standards. A corresponding MITOEAGLE proficiency training shall be developed and implemented. Feedback from the participants will lead to a final adjustment of the SOPs in the proficiency test, which will be made generally available.
Instrumental platforms
- Comparison of results between and within instrumental platforms: The reference protocols and proficiency test will not define the instrumental platform, but will allow a quantitative comparison of results obtained with specific equipment available in the participating laboratories.
Nomenclature
- Harmonization of nomenclature on mitochondrial respiratory states and control parameters: The logistics of development of a database requires application of strictly defined terms for all included variables. There is no general reference available upon which a consistent terminology on mitochondrial physiology and bioenergetics can be based. The MITOEAGLE consortium, therefore, has to accomplish the ambitious goal to unify and simplify the terminology in the field for the purpose of the QMS, which will lead to the development of recommendations for the use of a common terminology in mitochondrial physiological research. A publication will be prepared as an Open Access article which will be a milestone towards a unification of concepts and nomenclature.
Knowledge management system
- With an increase in data volume and complexity comes along the requirement for standardized report formats and digital accessibility of the data. Since such a database is still missing for mitochondrial physiology, the goal of WG1 is to establish an integrated and analytic environment linking mitochondrial respiration with external data (cell physiology, anthropometry, spiroergometry, lifestyle, genomics) in the context of physical fitness evaluation and clinical diagnostics. Databases will be established combining biological and clinical data comprising proprietary and publicly available datasets into one integrated solution. Data import and export functionalities will allow data upload and mapping to corresponding objects in the database, in this way generating valuable data and allowing for project-specific as well as cross-project data mining.
- The ability to formulate meta-studies in our approach is an important success factor: The platform can aggregate the project’s complete knowledge and provides the ability to make diagnostic profiling associated with diseases. In addition to functional data, other data types such as nutritional or environmental factors associated with disease prevention can be identified and aggregated into the MITOEAGLE Knowledge Management System.
- Querying the heterogeneous data types and result representation are the most important requirements of the knowledge platform besides to data integration and modelling. The application will feature different types of visualization techniques for all integrated data types. The database will be developed using the concept of multi-tier client-server architecture. Individual components are separated corresponding to their functionality. An upstream web server handles user requests, an application server performing business logic, and a back-end database system guaranties persistent data storage. All components are additionally separated by means of network security incorporating firewalls and are hosted in a secure environment. The platform administration ensures privacy regarding proprietary data by user rights policies.
- It will be the task of WG1 to continuously collect the input from all other WGs, compare, discuss and align SOPs as far as possible, and to finally develop recommendations for quality control, data reporting and data sharing beyond the published record. In addition, WG1 will develop concepts on Open Access and institutionalized service for data management, data mining and health-care conforming standards of data interpretation. Finally, it will provide a summary on strategic dissemination and an education programme for MITOEAGLE. The education programme will ensure that not only presently participating groups will adhere to the set standards but that also groups joining in at later points may smoothly fit into the community and help to further broaden and deepen our understanding on mitochondrial physiology in health and disease.
WG1 Participants
Country | City | Contact |
---|---|---|
Armenia | Yerevan | Trchounian K |
Australia | Southport | Vidimce Josif |
Australia | Clayton | Wolff Jonci Nikolai |
Australia | Burwood | Trewin Adam J |
Australia | Queensland | Radenkovic Filip |
Australia | Nedlands | Perks Kara L |
Australia | Victoria | Van Bergen Nicole J |
Australia | Geelong, Victoria | McKenzie Matthew |
Australia | Queensland | Holland Olivia J |
Australia | Sydney | Hoehn Kyle L |
Australia | Melbourne | Genders Amanda J |
Australia | Clayton Victoria | Alton Lesley |
Australia | Queensland | Dong Lan-Feng |
Australia | Melobourne | Botella Javier |
Australia | Melbourne, VIC | Muccini Anna Maria |
Australia | Melbourne | Coughlan Melinda T |
Australia | Sydney | Krycer James R |
Australia | Melbourne | Lionett S |
Australia | Syndey | Ton Riccardo |
Australia | Melbourne | Cassar Samantha |
Australia | Melbourne | Hewakapuge Sudinna |
Australia | Melbourne | Whitfield Jamie |
Australia | Newtown, Sydney | Stocker Roland |
Australia | Victoria | Winwood-Smith Hugh |
Australia | Queensland | Fisher Joshua J |
Australia | Nedlands | Filipovska Aleksandra |
Australia | Melbourne | Stepto Nigel K |
Australia | Melbourne | Ferri Alessandra |
Australia | Melbourne | Kuang Jujiao |
Australia | Melbourne | Saner Nicholas |
Australia | Parkville | Hardee Justin P |
Austria | Innsbruck | Gnaiger Erich |
Austria | Innsbruck | Hoppel Florian |
Austria | Innsbruck | Gallee Leon |
Austria | Innsbruck | Cardoso Luiza HD |
Austria | Innsbruck | Di Marcello Marco |
Austria | Aldrans | Haider Markus |
Austria | Innsbruck | Antunes Diana |
Austria | Innsbruck | Burtscher Martin |
Austria | Innsbruck | Nindl Veronika |
Austria | Innsbruck | Garcia-Souza Luiz F |
Austria | Innsbruck | Thapa Maheshwor |
Austria | Vienna | Kozlov Andrey V |
Austria | Innsbruck | Passrugger Manuela |
Austria | Kapferer Werner | |
Austria | Klosterneuburg | Sazanov Leonid A |
Austria | Innsbruck | Schartner Melanie |
Austria | Innsbruck | Karabatsiakis Alexander |
Austria | Innsbruck | Laner Verena |
Austria | Innsbruck | Doerrier Carolina |
Austria | Innsbruck | Klocker Helmut |
Austria | Innsbruck | Sobotka Ondrej |
Austria | Innsbruck | Meszaros Andras T |
Austria | Innsbruck | Keller Markus A |
Austria | Innsbruck | Goebel G |
Austria | Innsbruck | Schmitt Sabine |
Austria | Innsbruck | Bergmeister Lisa |
Austria | Innsbruck | Cecatto Cristiane |
Austria | Innsbruck | Plangger Mario |
Austria | Salzburg | Kofler B |
Austria | Innsbruck | Schwarzer Christoph |
Austria | Innsbruck | Perez Valencia Juan Alberto |
Belgium | Leuven, Flanders | Baekelandt V |
Belgium | Liege | Votion Dominique-Marie |
Belgium | Brussels | Sonveaux P |
Belgium | Namur | Arnould Thierry |
Belguim | Sart Tilman (Liège) | Kruse C |
Brasil | Porto Alegre | Haas Clarissa |
Brasilia | Limeira | Ropelle Eduardo R |
Brazil | Sao Paulo | Vercesi Anibal E |
Brazil | São Paulo | Kowaltowski Alicia J |
Brazil | Rio de Janeiro | Galina Antonio |
Brazil | Santa Maria - Rio Grande do Sul | Soares Felix Alexandre Antunes |
Brazil | Rio de Janeiro | Oliveira Marcus F |
Brazil | Santa Maria - RS | Goncalves Debora Farina |
Brazil | Rio de Janeiro | Vieyra Adalberto |
Brazil | Sao Paulo | Silber Ariel Mariano |
Brazil | São Paulo | Crispim Marcell |
Brazil | Jaboticabal | Oliveira Marcos Tulio |
Canada | Hamilton | Scott Graham R |
Canada | Montreal, Quebec | Bergdahl Andreas |
Canada | Saint John | Pulinilkunnil Thomas |
Canada | Quebec | Joseph Vincent |
Canada | Ottawa | Harper Mary-Ellen |
Canada | Guelph | Holloway Graham P |
Canada | Antigonish | Weir M |
Canada | Edmonton | Han Woo Hyun |
Canada | Montréal | Breton Sophie |
Canada | Winnipeg | Chowdhury Subir Roy |
Canada | Edmonton (Alberta) | Lemieux Helene |
Canada | Hamilton | Tarnopolsky Mark A |
Canada | Ottawa | Darveau Charles-A |
Canada | Moncton, NB | Pichaud Nicolas |
Canada | Vancouver | Bovard Josh |
Canada | Vancouver, BC | Tausan Daniel |
Canada | Rimouski | Munro Daniel |
Canada | Charlottetown | Adiele Reginald C |
Canada | Edmonton | Lucchinetti Eliana |
Canada | Edmonton | Zaugg Michael |
Canada | Rimouski | Blier Pierre U |
Chile | Coyhaique | Jana Prado Fabian |
China | Tianjin | Zhang Yong |
China | Pukou, Nanjing, | Gan Zhenji |
China | Fuzhou, Fujian Province | Chen Qi |
China | Xi`an | Liu Jiankang |
Croatia | Split | Ljubkovic M |
Croatia | Zagreb | Saric A |
Croatia | Zagreb | Rubelj Ivica |
Croatia | Zagreb | Curik I |
Czech Republic | Pardubice | Majtnerova Pavlina |
Czech Republic | Praha 2 | Zdrazilova Lucie |
Czech Republic | Vestec | Rohlena Jakub |
Czech Republic | Prague | Houstek Josef |
Czech Republic | Pardubice | Rousar Tomas |
Czech Republic | Hradec Kralove | Stankova Pavla |
Czech Republic | Hradec Kralove | Kucera Otto |
Czech Republic | Hradec Kralove | Endlicher Rene |
Czech Republic | Prague | Drahota Zdenek |
Czech Republic | Hradec Kralove | Cervinkova Zuzana |
Czech Republic | Ceske Budejovice | Zikova Alena |
Czech Republic | Praha 2 | Danhelovska Tereza |
Czech Republic | Pardubice | Handl Jiri |
Czech Republic | Vestec | Ezrova Zuzana |
Czech Republic | Vestec | Davidova Eliska |
Czech Republic | Prague | Krizova J |
Czech Republic | Prague 10 | Urban Tomas |
Czech Republic | Plzen -Severni predmesti | Jedlicka Jan |
Czech rep | Hradec Kralove | Falaye T |
Denmark | Copenhagen | Kraunsoee R |
Denmark | Copenhagen | Nielsen B |
Denmark | Copenhagen N | Maise Chroeis K |
Denmark | Copenhagen N | Mortensen OH |
Denmark | Aarhus N | Jespersen Nichlas Riise |
Denmark | Aarhus N | Boetker Hans Erik |
Denmark | Roskilde | Dalgaard Louise T |
Denmark | Hvidovre | Nehlin Jan O |
Denmark | Copenhagen | Soendergaard SD |
Denmark | Copenhagen | Gonzalez-Franquesa Alba |
Denmark | Copenhagen | Dela Flemming |
Denmark | Copenhagen N | Scheibye-Knudsen Morten |
Denmark | Copenhagen | Larsen Steen |
Egypt | Cairo | Abdel-Rahman Engy Ali |
Egypt | Cairo | Ali Sameh S |
Estonia | Tallinn | Puurand Marju |
Estonia | Tallinn | Vendelin Marko |
Estonia | Tallinn | Truu Laura |
Estonia | Tallinn | Laasmaa Martin |
Estonia | Tallinn | Klepinina Lyudmila |
Estonia | Tartu | Paju Kalju |
Estonia | Tallinn | Karro N |
Estonia | Tallinn | Tepp Kersti |
Estonia | Tallinn | Shevchuk Igor |
Estonia | Tallinn | Kaambre Tuuli |
Estonia | Tallinn | Klepinin Aleksandr |
Finland | Turku | Stier Antoine |
Finland | Jyvaeskylae | Kainulainen Heikki |
Finland | Helsinki | Suomalainen Wartiovaara Anu |
Finland | Helsinki | Jackson Christopher Benjamin |
France | Gueguen Naig | |
France | Bordeaux | Sarlak Saharnaz |
France | Angers | Spinazzi Marco |
France | Bordeaux | Amoedo Nivea Dias |
France | Paris | Armand Anne-Sophie |
France | Martinique | Cano Sanchez Maria Consolacion |
France | Montpellier | Wrutniak-Cabello Chantal |
France | Nouzilly | Collin-Chenot Anne |
France | Bordeaux cedex | Mourier A |
France | Bordeaux cedex | Rossignol Rodrigue |
France | Maisons-Alfort Cedex | Prola Alexandre |
France | Angers | Procaccio Vincent |
France | Martinique | Neviere Remi |
France | Paris | Bouillaud Frederic |
France | Tours | Dumas Jean-Francois |
France | Lille | Montaigne David |
France | Bordeaux | Mazat Jean-Pierre |
France | Plouzané | Salin Karine |
Germany | Ulm | Schaefer PM |
Germany | Duesseldorf | Roden Michael |
Germany | Marburg | Vogt Sebastian |
Germany | Marburg | Ramzan Rabia |
Germany | Düsseldorf | Goy C |
Germany | Cologne | Ho Dieu Hien |
Germany | Hannover | Das Anibh Martin |
Germany | Duesseldorf | Jelenik T |
Germany | Regensburg | Renner-Sattler Kathrin |
Germany | Cologne | Pesta Dominik |
Germany | Cologne | Trifunovic Aleksandra |
Germany | Wickert Anika | |
Germany | Frankfurt | Osiewacz Heinz D |
Germany | Neuherberg | Einer C |
Germany | Frankfurt | Warnsmann Verena |
Germany | Bremerhaven | Mark Felix Christopher |
Germany | Gießen | Grewal Rekha |
Germany | Wilhelmshaven | Salmon Pablo |
Germany | Freiburg | Schuele R |
Germany | Tuebingen | Kappler Lisa |
Germany | Frankfurt am Main | Viel Christian |
Germany | Rostock | Sokolova Inna |
Germany | Cologne | Wiesner Rudolf J |
Germany | Mainz | Methner Axel |
Germany | Magdeburg | Schoenfeld Peter |
Germany | Frankfurt | Hamann Andrea |
Germany | Giessen | Schulz Rainer |
Germany | Gießen | Silaidos Carmina |
Germany | Mainz | Maull Felicia |
Germany | Doermann Niklas | |
Germany | Giessen | Sommer Natascha |
Germany | Munich | Zischka Hans |
Germany | Jena | Szibor Marten |
Germany | Duesseldorf | Haendeler Judith |
Germany | Biberach an der Riß | Nold V |
Germany | Cologne | Maciej Sarah |
Germany | Duesseldorf | Piel Sarah |
Greece | Thessaloniki | Lazou Antigone |
Greece | Heraklion | Tavernarakis Nektarios |
Greece | Athens | Trougakos Ioannis P |
Greece | Athens | Gumeni Sentiljana |
Greece | 15771 | Andreadou Ioanna |
Hungary | Szeged | Ferdinandy Peter |
Hungary | Budapest | Komlodi Timea |
Hungary | Budapest | Tretter Laszlo |
Hungary | Budapest | Chinopoulos Christos |
Hungary | Szeged | Boros Mihaly |
Hungary | Debrecen | Verebne Tar K |
Hungary | Budapest | Horvath G |
India | New Delhi | Thakkar Himani |
India | Kollam | Suravajhala Prashanth |
India | Haryana | Chakrabarti Sasanka |
India | Varanasi | Sonkar Vijay K |
India | Hyderabad | Jha Rajan Kumar |
India | New Delhi | Vincent Vinnyfred |
India | Lucknow | Gayen Jiaur |
Iran | Tehran | Safaei Zahra |
Iran | Tehran | Shirazi Reza |
Ireland | Dublin | Porter Richard K |
Ireland | Dublin | O'Gorman Donal |
Israel | Zrifin | Sova Marina |
Israel | Beer Sheva | Mishmar Dan |
Israel | Jerusalem | Saada Reisch Ann |
Israel | Rishon Le Zion | Hachmo Yafit |
Italia | Udine | Salvadego Desy |
Italien | Padova PD | Szabo Ildiko |
Italy | Catania | De Pinto Vito |
Italy | Ancona | Battino Maurizio |
Italy | Padova | Bernardi Paolo |
Italy | Bologna | Paterlini S |
Italy | Udine | Grassi B |
Italy | Monserrato | Isola Raffaella |
Italy | Padova | Fernandez-Vizarra Erika |
Italy | Catania | Messina Angela |
Italy | Bari | Attimonelli M |
Italy | Rome | Scatena Roberto |
Italy | St. Lorenzen | Harrison David K |
Italy | Milan | Clementi Emilio |
Italy | Padova | Viscomi Carlo |
Italy | Bologna | Gasparre G |
Italy | Bologna | Porcelli AM |
Italy | Bolzano | Zanon Alessandra |
Italy | Catania | Magri Andrea |
Italy | Bologna | Genova Maria Luisa |
Italy | Milano | Bottoni P |
Italy | Bolzano/Bozen | Volani Chiara |
Japan | Sapporo | Matsumoto J |
Japan | Itabashi-ku Tokyo | Tanaka Masashi |
Kitzbühel | Innsbruck | Fischer Michael J |
Korea | Seoul | Pak Youngmi Kim |
Korea | Incheon | Kwak Hyo Bum |
Korea (South) | Seoul | Lee Hong Kyu |
Latvia | Riga | Dambrova Maija |
Latvia | Riga | Volska Kristine |
Latvia | Riga | Inashkina I |
Latvia | Riga | Jansone Baiba |
Latvia | Riga | Svalbe Baiba |
Latvia | Riga | Zvejniece Liga |
Latvia | Riga | Vilks Karlis |
Latvia | Riga | Liepins Edgars |
Latvia | Riga | Stelfa Gundega |
Luxembourg | Strassen | Devaux Yvan |
Malaysia | Kuala Lumpur | Hassan Hazirah |
Malta | Msida | Vella Joanna |
Mexico | Mexico City | Aparicio Trejo OE |
Mexico | Tlalpan | Rodriguez-Enriquez Sara |
México | San Pedro Garza García, Nuevo Leon | Garcia-Rivas Gerardo |
Netherlands | Maastricht | Grevendonk L |
Netherlands | GA Wageningen | Grefte Sander |
New Zealand | Auckland | Hickey Anthony J |
New Zealand | Auckland | Kaur Sarbjot |
New Zealand | Wellington | Berridge Michael V |
New Zealand | Auckland | Ward Marie Louise |
Norway | Trondheim | Lerfall Joergen |
Norway | Bergen | Tronstad Karl Johan |
Norway | Bergen | Blindheim Dan Filip |
Norway | Bergen | Pettersen Nitschke Ina Katrine |
Norway | Oslo | Eide L |
Norway | Trondheim | Rognmo O |
Norway | Bergen | Berge Rolf K |
Norway | Bergen | Dyrstad Sissel E |
Norway | Bergen | Roesland Gro Vatne |
Norway | Trondheim | Grill V |
Norway | Tromsoe | Boardman Neoma T |
Norway | Bergen | Haavik Jan |
Norway | Bergen | Hoel Fredrik |
Norway | Trondheim | Smenes Benedikte Therese |
Poland | Warsaw | Kampa Rafal Pawel |
Poland | Warsaw | Wieckowski Mariusz R |
Poland | Poznan | Jarmuszkiewicz Wieslawa |
Poland | Warsaw | Dymkowska Dorota |
Poland | Warsaw | Zablocki Krzysztof |
Poland | Lodz | Watala Cezary |
Poland | Warsaw | Bednarczyk Piotr |
Poland | Warsaw | Szewczyk Adam |
Poland | Lodz | Labieniec-Watala Magdalena |
Poland | Warsaw | Karkucinska-Wieckowska Agnieszka |
Poland | Warsaw | Sek Aleksandra |
Poland | Krakow | Dembinska-Kiec Aldona |
Poland | Gdańsk | Kaczor Jan Jacek |
Poland | Warsaw | Wasilewski M |
Poland | Warszawa | Jaskiewicz Anna |
Portugal | Coimbra | Rolo Anabela Pinto |
Portugal | Porto | Silva Ana Maria |
Portugal | Cantanhede | Amorim Ricardo |
Portugal | Coimbra | Palmeira Carlos |
Portugal | Coimbra | Duarte Filipe Valente |
Portugal | Aveiro | Ferreira Rita Maria P |
Portugal | Lisboa | Macedo Maria Paula |
Portugal | Coimbra | Oliveira Paulo J |
Portugal | Porto | Moreira Bruno P |
Portugal | Coimbra | Minuzzi Luciele M |
Portugal | Aveiro | Vitorino Rui Miguel Pinheiro |
Portugal | Porto | Oliveira Pedro Fontes |
Portugal | Porto | Alves Marco G |
Portugal | Coimbra | Dias Candida |
Portugal | Porto | Crisostomo Luis |
Portugal | Coimbra | Grilo Luis |
Portugal | Coimbra | Santos Diana |
Portugal | Porto | Beleza Jorge |
Portugal | Porto | Oliveira Jorge |
Portugal | Cantanhede | Teodoro Joao Soeiro |
Portugal | Cantanhede | Rodrigues Ana Sofia |
Qatar | Doha | Guarch Meritxell Espino |
Republic of Ghana | Cape Coast | Tei BN |
Republic of Korea | Busan | Kim Hyoung Kyu |
Republic of Korea | Yuseong-gu Daejeon | Quang Don T |
Republic of Korea | Busan | Han Jin |
Republic of Korea | Daejeon | Park SH |
Republic of Serbia | Belgrade | Krako Jakovljevic Nina |
Republic of Serbia | Novi Sad | Andric Silvana |
Republic of Serbia | Belgrade | Lalic Nebojsa M |
Russia | Moscow | Vinogradov Andrei D |
Russia | Moscow | Pavlova Nadia |
Russia | Yoshkar-Ola | Dubinin M |
Serbia | Belgrade | Misirkic Marjanovic Maja |
Serbia | Belgrade | Djordjevic M |
Serbia | Belgrade | Savkovic U |
Serbia | Belgrade | Mandic M |
Singapore | Singapore | Hausenloy Derek J |
Singapore | Singapore | Singh Brijesh Kumar |
Slovakia | Bratislava | Sumbalova Zuzana |
Slovakia | Košice | Cizmarova Beata |
Slovakia | Bratislava 45 | Ferko Miroslav |
Spain | Valencia | Casado Pinna Marta |
Spain | Barcelona | Perales Jose Carles |
Spain | A Coruña | Mayan MD |
Spain | Barcelona | Gil J |
Spain | A Coruña | Blanco FJ |
Spain | Granada | Quiles JL |
Spain | Madrid | Hernansanz-Agustin Pablo |
Spain | Madrid | Aragones Lopez J |
Spain | Sevilla | Bustos Matilde |
Spain | Madrid | Rial Eduardo |
Spain | Las Palmas de Gran Canaria | Calbet Jose AL |
Spain | Barcelona | Garcia-Roves Pablo Miguel |
Spain | Armilla (Granada) | Fernandez-Ortiz Marisol |
Spain | Barcelona | De la Torre Lara J |
Spain | Madrid | Moran M |
Spain | Salamanca | Almeida Angeles |
Spain | Barcelona | Bravo-Sagua Roberto |
Spain | Barcelona | Zorzano Antonio |
Spain | Barcelona | Goncalo Teixeira da Silva Rui |
Spain | Barcelona | Gama Perez Pau |
Spain | Granada | Hidalgo-Gutierrez A |
Sweden | Stockholm | Larsen Filip J |
Sweden | Stockholm | Shabalina Irina G |
Sweden | Lund | Ehinger Johannes K |
Sweden | Stockholm | Cardinale Daniele A |
Sweden | Gothenburg | Bhuvanachandran NS |
Sweden | Stockholm | Nedergaard J |
Switzerland | Lausanne | Amati Francesca |
Switzerland | Lausanne | Canto Alvarez Carles |
Switzerland | Lausanne | Lagarrigue Sylviane |
Switzerland | Lausanne | Gebara E |
Switzerland | Lausanne | Zalachoras I |
Switzerland | Lausanne | Weger M |
Switzerland | Lausanne | Zanou Nadege |
Switzerland | Zürich | Zaugg Kathrin |
Switzerland | Lausanne | Morato Fornaguera L |
Switzerland | Bern | Wyss RK |
Switzerland | Lausanne | Sandi Carmen |
Switzerland | Basel | Fornaro Mara |
Switzerland | Bern | Nuoffer Jean-Marc |
Switzerland | Basel | Bouitbir Jamal |
Switzerland | Lausanne | Place Nicolas |
Switzerland | Zurich | Gorr Thomas A |
Switzerland | Lausanne | Burtscher Johannes |
Switzerland | Lausanne | Donnelly Chris |
Taiwan | Changhua City | Wei Yau-Huei |
The Netherlands | BT Amsterdam | Wuest Rob CI |
The Netherlands | Maastricht | Nabben Miranda |
The Netherlands | Delft | McMillan Duncan GG |
The Netherlands | Maastricht | Schrauwen Patrick |
The Netherlands | Groningen | Bakker Barbara M |
UK | Canterbury, Kent | Gourlay Campbell W |
UK | London | Rodriguez Enrique |
UK | London | Bettinazzi Stefano |
UK | Cambridge | Brown Guy C |
UK | London | Lane Nick |
USA | Los Angeles | Kwast Kurt E |
USA | Corvallis | Robinson Matthew M |
USA | St. Louis | Abumrad Nada A |
USA | Winston-Salem | Tomar Dhanendra |
USA | Philadelphia | Jang David H |
USA | Puskarich Michael A | |
USA | Atlanta | Jang Young Charles |
USA | Little Rock | Parajuli Nirmala |
USA | Provo | Hancock Chad R |
USA | Aurora | Moreau Kerrie |
USA | Davis | Adams Sean H |
USA | Little Rock | Borsheim Elisabet |
USA | Little Rock | MacMillan-Crow Lee Ann |
USA | Aurora | Sparagna Genevieve C |
USA | Corvallis | Newsom Sean A |
USA | El Paso | Bajpeyi Sudip |
USA | Orlando | Coen Paul M |
USA | La Jolla | Schilling Jan M |
USA | Indianapolis | Brozinick Joseph T |
USA | Stillwater | Davis Michael S |
USA | Fort Collins | Li Puma Lance C |
USA | Winston-Salem | Ahn Bumsoo |
USA | Chicago | Dayanidhi Sudarshan |
USA | Corvallis | Batterson Philip M |
USA | Fairbanks | Coker Robert H |
USA | East Lansing | Bazil Jason N |
USA | Bethesda | Fessel Joshua Patrick |
USA | San Diego; La Jolla | Molina Anthony JA |
USA | Orlando | Pino Maria F |
USA | Berkeley | Williams Caroline M |
USA | St. Louis | Pietka Terri A |
USA | Iowa City | Wagner Brett A |
USA | Wakefield | Varricchio Frederick |
USA | La Jolla | Valentine Joseph Marco |
USA | Denver | Van Hove Johan |
USA | Jupiter | Trivigno Catherine |
USA | Birmingham | Moellering Douglas R |
USA | Pittsburgh | Jurczak Michael J |
USA | San Diego | Cannon Daniel T |
USA | Cleveland | Tandler Bernard |
USA | Orlando | Goodpaster Bret H |
USA | Louisville | Skolik Robert A |
USA | Columbus | Sims Carrie A |
USA | New York | Galkin Alexander |
USA | Denton | Dzialowski Edward M |
USA | Rochester | Nair K Sreekumaran |
USA | Ann Arbor | Tyrrell Daniel J |
USA | San Diego | Patel Hemal H |
USA | Gainesville | Leeuwenburgh Christiaan |
USA | Kansas City | Swerdlow Russell H |
USA | Baton Rouge | Hand Steven C |
USA | Miami | Fontanesi F |
USA | Milwaukee | Dash Ranjan K |
USA | Fayetteville | Rao RR |
USA | Louisville | Menze Michael A |
USA | East Lansing | Zhang Yizhu |
USA | Fairbanks | O'Brien Kristin |
USA | Warminster | Orynbayeva Zulfiya |
USA | Boston | Kristal Bruce S |
USA | Baltimore | Gonzalez-Freire Marta |
USA | Milwaukee | Kandel Sunil Mani |
USA | Rochester | Lanza Ian R |
USA | Baton Rouge | Irving Brian A |
USA | Fort Collins | Chicco Adam J |
USA | Bethesda | Sharma Pushpa |
USA | Durham | Li Pingan Andy |
USA | Pittsburgh | Prochownik Edward V |
USA | Greenville | Neufer P Darrell |
USA | Needham | Brown David A |
USA | Cleveland | Hoppel Charles L |
USA | Norfolk | Lai Nicola |
USA | Fayetteville | Iyer Shilpa |
USA | Nashville | Wasserman David H |
USA | Colorado Springs | Jacobs RA |
USA | Aurora | Ford Ellen |
USA | New Haven | Cavalcanti-de-Albuquerque Joao Paulo |
USA | Gainesville | Neyroud D |
USA | Portland | Garlid Keith D |
USA | Kansas City | Thyfault John P |
USA | Galveston | Durham William J |
USA | Orlando | Sparks Lauren M |
USA | Philadelphia | Falk Marni J |
USA | Akron | Cohen Bruce H |
USA | Iowa City | Chaurasia Bhagirath |
... further results |
WG1 Management
Tasks
- Opening of the database to the research public for data search and moderated entry of new data sets.
- Development of interlaboratory proficiency ring test, of recommendation/certification reports and of a MITOEAGLE proficiency training module (with WG5).
- Sample distribution, ring test experiments (MITOEAGLE-proficiency training on two different points of time, 6 months apart), data analysis by experimenters and MITOEAGLE-proficiency test manager.
- Reports and publication: Reports with specific results in comparison with an anonymous data summary, and joint publication. Individual reports may be complemented by recommendations, and are summarized in an informal certification report (move to WG5).
- Instrumental platform comparison with reference sample applying comparable measurements.
- Draft, dissemination, collection and final formulation of a manuscript on concepts and terminology of mitochondrial physiology (with WG5).
- Collect and discuss procedures and experimental protocols for the evaluation of mitochondrial capacities and create a library of protocols: A database comprising reference SUIT protocols, standard experimental media, and detailed instructions for OXPHOS analysis is assembled.
- MITOEAGLE data management system (DMS) development: The DMS for further data preprocessing. The DMS will enable management and analysis of instrument-specific and project-specific data.
- MITOEAGLE database (DB) development: DB will facilitate high-level project specific analysis as well as meta-analyses across projects, visualization of project specific data as well as integrative analyses of datasets for various proprietary and/or publicly available datasets.
- Testing of developed software components regarding functionality and usability, modification and implementation. Deployment for routine testing in the labs of our strategic partners.
- Data input: Upload of datasets generated through WG 2-4 in internal lab developments, results of the proficiency ring test, and during application module developments. In addition, the database will be populated with available proprietary and public datasets.
- Develop recommendations for quality control, data reporting and data sharing beyond the published record. Develop concepts on Open Access and institutionalized service for data management, data mining and health-care conforming standards of data interpretation (move to WG5).
- Provide a summary on strategic dissemination and an education programme for MITOEAGLE (move to WG5).
Milestones
- Data sets entered in standardized format and database opened for the public
- Protocols for ring-tests and standard analysis and report scheme defined
- MITOEAGLE proficiency training workshop finished (move to WG5)
- Analysis is completed and joint publication submitted (with WG5)
- Publication of paper on concepts and terminology (with WG5)
- Library of protocols online
- Publication of recommended protocols and procedures
- Guidelines for acquisition, evaluation and data documentation available (with WG5)
- MITOEAGLE data management system finished
- MITOEAGLE database ready for use
- Software modifications incorporated following extensive tests
- Publication of MITOEAGLE-KMP
- Recommendations on data acquisition, evaluation and management published (with WG5)
- Concepts on Open Access and institutionalized service for data management, data mining and health-care conforming standards of data interpretation published (move to WG5)
Deliverables
- Guidelines for future research and recommendations for the evaluation of respiratory characteristics in human respirometric samples (with WG5)
- A database on mt fitness evaluated with human cells and tissues
- Training of researchers towards improved reproducibility of sample preparation and respirometric evaluation (move to WG5)
- Qualitative and quantitative evaluation of results obtained in ongoing studies by comparison with a reference sample
- An expanded MITOEAGLE database
- A joint publication documenting the results of the MITOEAGLE-PT (with WG5)
- A multi-authored publication towards a unification of concepts and nomenclature in mitochondrial physiology (with WG5)
- A library of protocols for sample preparation and examination by respirometry
- A multi-authored publication with recommendations of comparable standard protocols and procedures (with WG5)
- Guidelines as to the optimum use of a reference sample for determination of respiration and related parameters of interest
- The MITOEAGLE data management system
- A continuously updated MITOEAGLE database for the use as reference data and guideline towards application of optimized protocols, ultimately leading towards standardization.
- A joint publication on the use of the MITOEAGLE database and management system (with WG5)
- An education programme for the use of MITOEAGLE database and management system (move to WG5).
WG1 Feedback
- We are mostly interested in two WG: (1) Since we are working mostly with heart and muscles, we are interested in WG 2 - MITOEAGLE data repository in muscle and other tissues. (2) WG 1 - Standard operating procedures and user requirement document: Protocols, terminology, documentation. Since we have an expertise in fatty acid metabolism, we are specially interested in experimental protocols for the evaluation of mitochondrial capacities regarding fatty acid metabolism. Of course our parts of the WG are also in our interest. - Marina Makrecka-Kuka, Edgars Liepinsh (2016).
- My role in Mito-EAGLE would be mainly in WG 2 and 4, with a focus on skeletal and cardiac muscle and cultured cardiomyocytes and endothelial cells. I would particularly like to bridge the gap between the group leaders and young investigators, trying to represent the young people who are the active users of the OROBOROS equipment. This will fit in nicely with the goals in WG1. ... Major congratulations on obtaining this beautiful collaborative grant! Very glad to see this network getting the recognition it deserves! Looking forward to being part of this! - Rob C Wuest (2016).
- Please, find enclosed my declaration to participate with my team in the following workgroups: WG1, WG2, WG3, WG4. We are now involved and have been in not a distant past – in studying of the following cell /tissue types:
- Blood cells, with particular emphasis on platelets and lymphocytes – isolated from animals and humans.
- Endothelial cells, fibroblasts, neurocytes (neuroblastoma), astrocytes, cancer cell lines – predominantly cultured.
- Hepatocytes, cardiomyocytes, cell isolated from brain – isolated from lab animals. - Cezary Watala (2016).
- I have already contacted the portuguese CNC to nominate me for the MC of theaction. Concerning my active participation, taking into account the type of work we do in my lab I could be included in all WGs. As you know we work with mitochondria from liver, muscle and fat, as well as cultured cells from different origins (WG2-4). The elaboration of SOPs (WG1) we can certainly contribute (see the book I edited on Mitochondrial Bioenergetics and in which you contribute a chapter). I believe that everybody can be part of the WG5, but as the coordinators of the action, you and Sandra should decide what is best for the success of the action. - Carlos Palmeira (2016).
- I would propose my participation in the WG1: Standard operating procedures and user requirement document: Protocols, terminology, documentation, considering that the goal of WG1 is to establish an integrated and analytic environment linking mitochondrial respiration with external data (cell physiology, anthropometry, spiroergometry, lifestyle, genomics) in the context of physical fitness evaluation and clinical diagnostics. I could give my active contribution in the phase of making platform that will aggregate the Action’s complete knowledge and provides the ability to make diagnostic profiling associated with diseases in particular tasks:
- Develop reccomendations for quality control, data reporting and data sharing beyond the published record. Develop concepts on Open Access and institutionalised service for data management, data mining and health-care conforming standards of data interpretation
- Provide a summary on strategic dissemination and an education programme for MITOEAGLE.
- Considering that we are in process of buying the oxygraph, thus broading capacities of my lab, I would be happy to open the door of my new lab for the innovative inter-laboratory ring test experiments and together with my team contribute in that phase of the MITOEAGLE Action. I look forward to the establishment of MitoGlobal EAGLE network where, as stated in the MoU, researchers will collaborate on mapping mitochondrial physiology and medicine. The creation of such a unique well-coordinated network of senior researchers and young investigators will definitely put an added value to existing data and publications, will increase quality and validity of the research, will bring new possibilities of data sharing and data mining and will hopefully bring us to the era of more economical research by saving resources through standardised and harmnonised protocols, where the measuremnts will not be wasted due to lack of comparability. - Nebojsa Lalic (2016).
- Our research interest is oriented to reproductive endocrinology; especially to mechanisms that govern steroidogenesis of testicular Leydig cells in disturbed homeostasis. We use several in vivo models:
- stress -/+ systemic or intratesticular blockade of different stress receptors (alpha1ADR; betaADR; GR)
- hypogonadal-hypogonadism -/+ testosterone-replacement therapy
- androgens anabolics treatment -/+ blockade od AR
- aged rats -/+ Viagra-treatment
- biological clock - Silvana Andric (2016).
- I took a look to the MoU and the different working groups. You know well my research focus and having this into account I am interested in actions related to Working group 1 (Respirometric reference protocols, Interlaboratory proficiency test, Instrumental platforms). Working group 2 and 3: I have generated multiple mitochondrial respiration data sets in different mouse tissues (mainly in skeletal muscle, hypothalamus, liver and white adipose tissue). Currently we are also performing these assays in white adipose tissue from human patients. Hence I am interested in all milestones of WG 2 and 3. WG 5 is related to WG 1 and as you know I have interest in training, dissemination and also together with my collaborators (if needed) we could help with data sets and a data interpretation. Overall, I have a general interest on this Action and my active role in MITOEAGLE could be discussed. I could be of help participating in some of the tasks where my expertise and interest fit best. We will be forming and interdisciplinary research group with four group leaders: JC Perales, R Guimera, M Sales-Pardo and PM Garcia-Roves with two different affiliations, University of Barcelona (JCP and PMG-R - Energy metabolism) and University Rovira I Virgili (RG and MS – Data base, statistics, data mining). - Pablo M Garcia-Roves (2016).
- I will be pleased to contribute to the tasks of WG1 by developing interlaboratory proficiency tests and instrumental platform comparison. - Maria Luisa Genova (2016).
Projects in progress
- Interlaboratory proficiency ring test
- Testing of software components
- Concepts and terminology on mitochondrial physiology
- Procedures and experimental protocols for the evaluation of mitochondrial capacities, library of protocols
- Data management system (DMS)
- Database
Next steps
- Best practices for implementation of analytical and process standards.
References
- General requirements for the competence of testing and calibration laboratories » ISO/IEC 17025:2005