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High signal at zero oxygen |
MitoPedia O2k and high-resolution respirometry:
O2k-Open Support
Description
A high signal at zero oxygen may be observed during zero calibration (R0). First, check the quality of the dithionite solution. The following instructions show how to distinguish between a defective sensor head and an electrical leak current.
Reference: Zero calibration, OroboPOS-Connector
Leak current test
O2k-series J and XB
O2k-series D-I and XA
- 1. Unscrew the POS from the POS-Connector and unplug the POS-Connector from the O2k-main unit.
- 2. Switch on the O2k and start DatLab.
- 3. Record the signal for 10 min.
- 4. Connect the POS-Connector to the O2k-main unit without the POS. Set an event in DatLab called "POS-Connector".
- 5. Record the signal for 10 min.
- 6. Remove the membrane from the POS and wash the cathode and anode with distilled water.
- 7. Screw the POS to the POS-Connector. Set an event in DatLab called "POS".
- 8. Record the signal for 10 min.
- 9. Send us the file.
O2k-series A-C
- Please start with the POS-Connector already connected to the main unit and proceed with step 5.
References
- Example: First, the OroboPOS-Connector and the POS were disassembled and disconnected from the main unit. Second, the OroboPOS-Connector was connected to the O2k-Main unit without the OroboPOS. Third, the OroboPOS was connected to the OroboPOS-Connector without membranes. After each step, the O2 raw signal [V] was recorded for 10 min. The marks were set to the O2 raw signal [V] to evaluate the zero current.
- Figure 1. High zero current test. Upper panel: Zero current is in the range of Β±0.00099 V. Lower panel: Zero current is higher than 0.00099 V when the OroboPOS-Connector is connected to the O2k-Main unit and when the OroboPOS is connected to the OroboPOS-Connector, indicating a potential electrical problem with both the OroboPOS-Connector and the OroboPOS.
Keywords
- Bioblast links: Oxygen signal - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
- Specific
- Β» Oxygen signal
- Β» O2k signal line
- Specific
- O2k-Procedures
- MiPNet O2k-Procedures
- General
- Β» Oxygen, dioxygen, O2
- Β» Oxygen calibration - DatLab
- Β» Oxygen solubility
- Β» Oxygen solubility factor
- Β» Oxygen pressure
- Β» Concentration
- Β» Activity
- Β» Pressure - Pascal
- Β» Barometric pressure
- Β» High-resolution respirometry
- Β» OroboPOS
- Β» Polarographic oxygen sensor
- Β» MitoFit Quality Control System
- Β» Sensitivity
- General
- Other keyword lists
MitoPedia O2k and high-resolution respirometry: O2k hardware, O2k-Open Support, O2k-Respirometry