Template:Current week ecosystem agenda: Difference between revisions

Latest revision as of 09:37, 21 May 2024

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-» A future for Ukraine is the future of its science

2024 Week 21

May-20 Mo

How do I determine the optimal oligomycin concentration for my assay?

Perform the oligomycin titration test, and adjust your oligomycin concentration to minimize the inhibition of ET capacity.
- »Oligomycin titration test«
- communicated by »Baglivo Eleonora« and »Schmitt Sabine«

May-21 Tu

May-22 We - International Day for Biological Diversity

"We have identified a circadian clock phase-dependent change in Complex I coupling efficiency, demonstrating for the first time a mitochondria–clock interaction in the hooded seal."

Ciccone C, Kante F, Folkow LP, Hazlerigg DG, West AC, Wood SH (2024) Circadian coupling of mitochondria in a deep-diving mammal. »Bioblast link«

from the O2k-Network »NO Tromsoe Wood SH«

May-23 Th

May-24 Fr

Next Week

2024 Week 22

May-27 Mo

Same experimental conditions, but different O₂ fluxes in the left and right chambers?

Check for instrumental and operational factors that could be causing O₂ flux differences between the O2k-chambers.
- »Different O2 fluxes in left and right chamber«
- communicated by »Timon-Gomez Alba« and »Baglivo Eleonora«

May-28 Tu

May-29 We

May-30 Th

"Harmonization of isolation protocols yielding intact and enzymatically active mitochondria are critical to assess mitochondrial respiratory function"

- »MiPNet20.06 Isolation mouse heart mitochondria«
- communicated by »Cardoso Luiza« and »Leo Elettra«

May-31 Fr

@@ Line 1: / Line 1: @@
 {{Template:Science for Ukraine}}
-Week 08
-* '''Feb-19 Mo'''
+Week 21
+* '''May-20 Mo'''
 :{|
-| width="110" align="center" | [[Image:O2k-Feedback.jpg|60px|link=https://wiki.oroboros.at/index.php/O2k-Feedback |O2k-Feedback]]
+| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
-|''' ''“We just published this paper on [https://academic.oup.com/biomedgerontology/advance-article/doi/10.1093/gerona/glad283/7502466 SOMMA], possibly one of the largest aging studies involving permeabilized muscle energetics. Couldn't have done it without the training your teams provided.”'''''<br>
+|''' How do I determine the optimal oligomycin concentration for my assay?'''<br>
-'''[[Kramer Philip A]]''' from the O2k-Network Lab  »[[US NC Winston-Salem Kramer PA]]«<br>
+Perform the oligomycin titration test, and adjust your oligomycin concentration to minimize the inhibition of ET capacity. <br>
-- »'''[https://wiki.oroboros.at/index.php/O2k-Feedback#More_O2k-Feedback| O2k-Feedback]'''«<br>
+- »'''[https://wiki.oroboros.at/index.php/Oligomycin#Omy_titration_test Oligomycin titration test]'''« <br>
-|}
+- communicated by »[[Baglivo Eleonora]]« and »[[Schmitt Sabine]]«
+|}
 ----
-* '''Feb-20 Tu'''
+* '''May-21 Tu'''
 ----
-* '''Feb-21 We'''
+* '''May-22 We''' - International Day for Biological Diversity
-----
-* '''Feb-22 Th'''
 :{|
-| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
+| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
-|'''What to do if the glass of the O2k-chamber starts to become opaque?'''<br>
+|'''"We have identified a circadian clock phase-dependent change in Complex I coupling efficiency, demonstrating for the first time a mitochondria–clock interaction in the hooded seal."''' <br>
-"Turbidity on the glass wall may be caused by precipitated protein. Remove the chambers from the O2k and with the O2k-stirrers removed, immerse in a beaker filled with HCl (10 M) overnight under a hood." <br>
+* Ciccone C, Kante F, Folkow LP, Hazlerigg DG, West AC, Wood SH (2024) Circadian coupling of mitochondria in a deep-diving mammal. [[Ciccone 2024 J Exp Biol|»Bioblast link«]]
--»'''[[MiPNet19.03_O2k-cleaning_and_ISS]]'''«<br>
+:from the O2k-Network »[[NO Tromsoe Wood SH]]«
-- communicated by »[[Cardoso Luiza]]« and »[[Leo Elettra]]«
 |}
 ----
-* '''Feb-23 Fr'''
+* '''May-23 Th'''
+----
+* '''May-24 Fr'''
 ----
 '''<big>Next Week</big>'''
-Week 09
+Week 22
-* '''Feb-26 Mo'''
+* '''May-27 Mo'''
 :{|
-| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
+| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Info: take care]]
-|''' ATP synthase = phosphorylation system? '''<br>
+|'''Same experimental conditions, but different O<sub>2</sub> fluxes in the left and right chambers?'''<br>
-No, the phosphorylation system consists of adenine nucleotide translocase, phosphate carrier, and ATP synthase, which carry out the steps in the phosphorylation pathway.  <br>
+Check for instrumental and operational factors that could be causing O<sub>2</sub> flux differences between the O2k-chambers.
-- »'''[[Phosphorylation pathway]]'''«, »'''[https://www.bioblast.at/index.php/Gnaiger_2020_BEC_MitoPathways Mitochondrial Pathways: Chapter 2.2]'''« <br>
+<br>
-- communicated by »[[Schmitt Sabine]]« and »[[Timon-Gomez Alba]]«
+- »'''[[Different O2 fluxes in left and right chamber]]'''«<br>
+- communicated by »[[Timon-Gomez Alba]]« and »[[Baglivo Eleonora]]«
 |}
 ----
-* '''Feb-27 Tu'''
+* '''May-28 Tu'''
 ----
-* '''Feb-28 We'''
+* '''May-29 We'''
 ----
-* '''Feb-29 Th'''
+* '''May-30 Th'''
 :{|
-| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
+| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
-|'''"Bed rest results in a decrease in skeletal muscle mitochondrial respiratory capacity, mainly as a result of an observed decrease in mitochondrial content"''' <br>
+|''' "Harmonization of isolation protocols yielding intact and enzymatically active mitochondria are critical to assess mitochondrial respiratory function"'''<br>
-* Noone J, Damiot A, Kenny H, Chery I, Zahariev A, Normand S, Crampes F, de Glisezinski I, Rochfort KD, Laurens C, Bareille MP, Simon C, Bergouignan A, Blanc S, O'Gorman DJ (2023) The impact of 60 days of -6° head down tilt bed rest on mitochondrial content, respiration and regulators of mitochondrial dynamics. [[Noone 2023 J Physiol|»Bioblast link«]]
+- »'''[[MiPNet20.06_IsolationMouseHeart-mt|MiPNet20.06 Isolation mouse heart mitochondria]]'''«<br>
-:from the O2k-Networks »[[US FL Orlando Goodpaster BH]]« , »[[FR Strasbourg Blanc S]]« , »[[IE Dublin O Gorman D]]«
+- communicated by »[[Cardoso Luiza]]« and »[[Leo Elettra]]«
 |}
 ----
-* '''Mar-01 Fr'''
+* '''May-31 Fr'''
 ----