Ciccone 2024 J Exp Biol
| Ciccone C, Kante F, Folkow LP, Hazlerigg DG, West AC, Wood SH (2024) Circadian coupling of mitochondria in a deep-diving mammal. J Exp Biol 227:jeb24699. https://doi.org/10.1242/jeb.246990 |
Ciccone Chiara, Kante Fayiri, Folkow Lars P, Hazlerigg David G, West Alexander C, Wood Shona H (2024) J Exp Biol
Abstract: Regulation of mitochondrial oxidative phosphorylation is essential to match energy supply to changing cellular energy demands, and to cope with periods of hypoxia. Recent work implicates the circadian molecular clock in control of mitochondrial function and hypoxia sensing. Because diving mammals experience intermittent episodes of severe hypoxia, with diel patterning in dive depth and duration, it is interesting to consider circadian-mitochondrial interaction in this group. Here, we demonstrate that the hooded seal (Cystophora cristata), a deep-diving Arctic pinniped, shows strong daily patterning of diving behaviour in the wild. Cultures of hooded seal skin fibroblasts exhibit robust circadian oscillation of the core clock genes per2 and arntl. In liver tissue collected from captive hooded seals, expression of arntl was some 4-fold higher in the middle of the night than in the middle of the day. To explore the clock-mitochondria relationship, we measured the mitochondrial oxygen consumption in synchronized hooded seal skin fibroblasts and found a circadian variation in mitochondrial activity, with higher coupling efficiency of complex I coinciding with the trough of arntl expression. These results open the way for further studies of circadian-hypoxia interactions in pinnipeds during diving. • Keywords: Arctic, Biological clock, Hooded seal, Hypoxia, Mitochondrial respiration, Pinniped • Bioblast editor: Plangger M
Labels: MiParea: Respiration, Comparative MiP;environmental MiP
Stress:Hypoxia Organism: Other mammals Tissue;cell: Fibroblast Preparation: Permeabilized cells, Intact cells
Coupling state: LEAK, ROUTINE, OXPHOS, ET
Pathway: N, S, NS
HRR: Oxygraph-2k
2024-04
