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Latest revision as of 09:37, 21 May 2024

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-» A future for Ukraine is the future of its science

2024 Week 21

May-20 Mo

How do I determine the optimal oligomycin concentration for my assay?

Perform the oligomycin titration test, and adjust your oligomycin concentration to minimize the inhibition of ET capacity.
- »Oligomycin titration test«
- communicated by »Baglivo Eleonora« and »Schmitt Sabine«

May-21 Tu

May-22 We - International Day for Biological Diversity

"We have identified a circadian clock phase-dependent change in Complex I coupling efficiency, demonstrating for the first time a mitochondria–clock interaction in the hooded seal."

Ciccone C, Kante F, Folkow LP, Hazlerigg DG, West AC, Wood SH (2024) Circadian coupling of mitochondria in a deep-diving mammal. »Bioblast link«

from the O2k-Network »NO Tromsoe Wood SH«

May-23 Th

May-24 Fr

Next Week

2024 Week 22

May-27 Mo

Same experimental conditions, but different O₂ fluxes in the left and right chambers?

Check for instrumental and operational factors that could be causing O₂ flux differences between the O2k-chambers.
- »Different O2 fluxes in left and right chamber«
- communicated by »Timon-Gomez Alba« and »Baglivo Eleonora«

May-28 Tu

May-29 We

May-30 Th

"Harmonization of isolation protocols yielding intact and enzymatically active mitochondria are critical to assess mitochondrial respiratory function"

- »MiPNet20.06 Isolation mouse heart mitochondria«
- communicated by »Cardoso Luiza« and »Leo Elettra«

May-31 Fr

@@ Line 1: / Line 1: @@
 {{Template:Science for Ukraine}}
-Week 07
-* '''Feb-12 Mo'''
+Week 21
+* '''May-20 Mo'''
 :{|
 | width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
-|''' What is the O<sub>2</sub> solubility factor of the medium (''F''<sub>M</sub>) and why is it important? '''<br>
+|''' How do I determine the optimal oligomycin concentration for my assay?'''<br>
-''F''<sub>M</sub> expresses the effect of the salt concentration on oxygen solubility relative to pure water, and varies depending on the temperature and composition of the medium. The correct ''F''<sub>M</sub> needs to be entered in DatLab when performing an air calibration. <br>
+Perform the oligomycin titration test, and adjust your oligomycin concentration to minimize the inhibition of ET capacity. <br>
-- »'''[[Oxygen solubility factor]]'''« , »'''[[Oxygen_calibration_-_DatLab#Air_calibration_in_DatLab|Air calibration in DatLab]]'''« <br>
+- »'''[https://wiki.oroboros.at/index.php/Oligomycin#Omy_titration_test Oligomycin titration test]'''« <br>
-- communicated by »[[Baglivo Eleonora]]« and »[[Timon-Gomez Alba]]«
+- communicated by »[[Baglivo Eleonora]]« and »[[Schmitt Sabine]]«
 |}
-----
-* '''Feb-13 Tu'''
 ----
-* '''Feb-14 We'''
+* '''May-21 Tu'''
 ----
-* '''Feb-15 Th''' - International Childhood Cancer Day
+* '''May-22 We''' - International Day for Biological Diversity
 :{|
 | width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
-|'''"Deleterious mtDNA mutations contribute significantly to the tumorigenesis of pediatric cancers"''' <br>
+|'''"We have identified a circadian clock phase-dependent change in Complex I coupling efficiency, demonstrating for the first time a mitochondria–clock interaction in the hooded seal."''' <br>
-* Triska P, Kaneva K, Merkurjev D, Sohail N, Falk MJ, Triche TJ Jr, Biegel JA, Gai X (2019) Landscape of germline and somatic mitochondrial DNA mutations in pediatric malignancies. [[Triska 2019 Cancer Res|»Bioblast link«]]
+* Ciccone C, Kante F, Folkow LP, Hazlerigg DG, West AC, Wood SH (2024) Circadian coupling of mitochondria in a deep-diving mammal. [[Ciccone 2024 J Exp Biol|»Bioblast link«]]
-:from the O2k-Network »[[US_PA_Philadelphia_Falk_MJ]]«
+:from the O2k-Network »[[NO Tromsoe Wood SH]]«
 |}
 ----
-* '''Feb-16 Fr'''
+* '''May-23 Th'''
+----
+* '''May-24 Fr'''
 ----
 '''<big>Next Week</big>'''
-Week 08
+Week 22
-* '''Feb-19 Mo'''
+* '''May-27 Mo'''
 :{|
-| width="110" align="center" | [[Image:O2k-Feedback.jpg|60px|link=https://wiki.oroboros.at/index.php/O2k-Feedback |O2k-Feedback]]
+| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Info: take care]]
-|''' ''“We just published this paper on [https://academic.oup.com/biomedgerontology/advance-article/doi/10.1093/gerona/glad283/7502466 SOMMA], possibly one of the largest aging studies involving permeabilized muscle energetics. Couldn't have done it without the training your teams provided.”'''''<br>
+|'''Same experimental conditions, but different O<sub>2</sub> fluxes in the left and right chambers?'''<br>
-'''[[Kramer Philip A]]''' from the O2k-Network Lab  »[[US NC Winston-Salem Kramer PA]]«<br>
+Check for instrumental and operational factors that could be causing O<sub>2</sub> flux differences between the O2k-chambers.
-- »'''[https://wiki.oroboros.at/index.php/O2k-Feedback#More_O2k-Feedback| O2k-Feedback]'''«<br>
+<br>
+- »'''[[Different O2 fluxes in left and right chamber]]'''«<br>
+- communicated by »[[Timon-Gomez Alba]]« and »[[Baglivo Eleonora]]«
 |}
 ----
-* '''Feb-20 Tu'''
+* '''May-28 Tu'''
 ----
-* '''Feb-21 We'''
+* '''May-29 We'''
 ----
-* '''Feb-22 Th'''
+* '''May-30 Th'''
 :{|
 | width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
-|'''What to do if the glass of the O2k-chamber starts to become opaque?'''<br>
+|''' "Harmonization of isolation protocols yielding intact and enzymatically active mitochondria are critical to assess mitochondrial respiratory function"'''<br>
-"Turbidity on the glass wall may be caused by precipitated protein. Remove the chambers from the O2k and with the O2k-stirrers removed, immerse in a beaker filled with HCl (10 M) overnight under a hood." <br>
+- »'''[[MiPNet20.06_IsolationMouseHeart-mt|MiPNet20.06 Isolation mouse heart mitochondria]]'''«<br>
--»'''[[MiPNet19.03_O2k-cleaning_and_ISS]]'''«<br>
 - communicated by »[[Cardoso Luiza]]« and »[[Leo Elettra]]«
 |}
 ----
-* '''Feb-23 Fr'''
+* '''May-31 Fr'''
 ----