High signal at zero oxygen: Difference between revisions
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{{Technical support}} | {{Technical support}} | ||
{{MitoPedia without banner | {{MitoPedia without banner | ||
|abbr= | |abbr= | ||
|description=A '''high signal at zero oxygen''' may be observed during [[zero calibration]] (R0). The following instructions show how to | |description=A '''high signal at zero oxygen''' may be observed during [[zero calibration]] (R0). First, check the quality of the [[dithionite]] solution. The following instructions show how to distinguish between a defective sensor head and an electrical leak current. | ||
|info= [[Zero_calibration | Zero calibration]], [[OroboPOS-Connector_MitoPedia#Cleaning_the_electrical_connections | OroboPOS-Connector]] | |info= [[Zero_calibration | Zero calibration]], [[OroboPOS-Connector_MitoPedia#Cleaning_the_electrical_connections | OroboPOS-Connector]] | ||
}} | }} | ||
== | __TOC__ | ||
== Leak current test== | |||
=== O2k-series J and XB === | === O2k-series J and XB === | ||
::::# Unplug the [[SmartPOS]] from the O2k-main unit. | |||
::::# Remove the membrane from the SmartPOS and wash the cathode and anode with distilled water. | |||
::::# Switch on the O2k and start DatLab. | |||
::::# Record the signal for 10 min. | |||
::::# Connect the SmartPOS to the O2k-main unit. Set an event in DatLab called "SmartPOS". | |||
::::# Record the signal for 10 min. | |||
::::# The leak current of each individual component should be stable and below 0.001 Β΅A (DatLab 8). If the SmartPOS is giving a high leak current a sensor service frequently solves the problem. If you have any uncertainties, please forward the file to the [https://www.oroboros.at/index.php/o2k-technical-support/ technical support]. | |||
=== O2k-series D-I and XA === | === O2k-series D-I and XA === | ||
:::: | ::::# Unplug the [[POS-Connector]] from the O2k-main unit and unscrew the [[OroboPOS]] from the [[POS-Connector]]. Screw the POS to the blue part of the perspex housing. | ||
:::: | ::::# Remove the membrane from the POS and wash the cathode and anode with distilled water. | ||
:::: | ::::# Switch on the O2k and start DatLab. | ||
:::: | ::::# Record the signal for 10 min. | ||
:::: | ::::# Connect the POS-Connector to the O2k-main unit without the POS. Set an event in DatLab called "POS-Connector". | ||
:::: | ::::# Record the signal for 10 min. | ||
::::# Screw the POS to the POS-Connector. Set an event in DatLab called "POS". | |||
:::: | ::::# Record the signal for 10 min. | ||
:::: | ::::# The leak current of each individual component should be stable and below 0.001 Β΅A (DatLab 8) or V (DatLab 7 and previous versions). DependingΒ which component is giving a high leak current, a sensor service, followed by [https://wiki.oroboros.at/index.php/OroboPOS-Connector#Cleaning_the_electrical_connections cleaning the electrical connections] and drying the OroboPOS (without membrane) or the POS-Connector in an oven at up to 60 Β°C for 1-2 h frequently solves the problem. If you have any uncertainties, please forward the file to the [https://www.oroboros.at/index.php/o2k-technical-support/ technical support]. | ||
Β | |||
=== O2k-series A-C === | === O2k-series A-C === | ||
:::: | ::::: Plugging or unplugging of the POS-Connector while the main unit is switched on could damage the POS-Connector of O2k-series A-C. Therefore, the following should be started with the O2k switched off. | ||
::::# Unplug the [[POS-Connector]] from the O2k-main unit and unscrew the [[OroboPOS]] from the [[POS-Connector]]. Screw the POS to the blue part of the perspex housing. | |||
::::# Remove the membrane from the POS and wash the cathode and anode with distilled water. | |||
::::# Plug the POS-Connector to the main unit. | |||
::::# Switch on the O2k and start DatLab. | |||
::::# Record the signal for 10 min. | |||
::::# Screw the POS to the POS-Connector. Set an event in DatLab called "POS". | |||
::::# Record the signal for 10 min. | |||
::::# The leak current of each individual component should be stable and below 0.001 V (DatLab 7 and previous versions). DependingΒ which component is giving a high leak current, a sensor service, followed by [https://wiki.oroboros.at/index.php/OroboPOS-Connector#Cleaning_the_electrical_connections cleaning the electrical connections] and drying the OroboPOS (without membrane) or the POS-Connector in an oven at up to 60 Β°C for 1-2 h frequently solves the problem. If you have any uncertainties, please forward the file to the [https://www.oroboros.at/index.php/o2k-technical-support/ technical support]. | |||
=== References === | |||
::::::[1] [https://wiki.oroboros.at/index.php/OroboPOS-Connector_MitoPedia#Cleaning_the_electrical_connections Cleaning the electrical connections] | ::::::[1] [https://wiki.oroboros.at/index.php/OroboPOS-Connector_MitoPedia#Cleaning_the_electrical_connections Cleaning the electrical connections] | ||
::::::[2] [[MiPNet19.18B POS-service]] | ::::::[2] [[MiPNet19.18B POS-service]] | ||
Line 28: | Line 44: | ||
::::::[4] [[MiPNet14.06 Instrumental O2 background]] | ::::::[4] [[MiPNet14.06 Instrumental O2 background]] | ||
::: | === Example === | ||
::: First, the OroboPOS-Connector and the POS were disassembled and disconnected from the main unit. Second, the OroboPOS-Connector was connected to the O2k-Main unit without the OroboPOS. Third, the OroboPOS was connected to the OroboPOS-Connector without membranes. After each step, the O<sub>2</sub> raw signal [V] was recorded for 10 min. The marks were set to the O<sub>2</sub> raw signal [V] to evaluate the leak current. | |||
::::[[File:High zero current good example.png|700px]] | ::::[[File:High zero current good example.png|700px]] | ||
::::[[File:High zero current test bad exmaple.png|700px]] | ::::[[File:High zero current test bad exmaple.png|700px]] | ||
::::'''Figure 1.''' | ::::'''Figure 1.''' Leak current test.Β '''Upper panel:''' Leak current is in the range of Β±0.00099 V. '''Lower panel:''' Leak current is higher than 0.00099 V when the OroboPOS-Connector is connected to the O2k-Main unit and when the OroboPOS is connected to the OroboPOS-Connector, indicating a potential electrical problem of both, the OroboPOS-Connector, and the OroboPOS. | ||
== Keywords == | == Keywords == |
Latest revision as of 10:47, 5 April 2024
High signal at zero oxygen |
MitoPedia O2k and high-resolution respirometry:
O2k-Open Support
Description
A high signal at zero oxygen may be observed during zero calibration (R0). First, check the quality of the dithionite solution. The following instructions show how to distinguish between a defective sensor head and an electrical leak current.
Reference: Zero calibration, OroboPOS-Connector
Leak current test
O2k-series J and XB
- Unplug the SmartPOS from the O2k-main unit.
- Remove the membrane from the SmartPOS and wash the cathode and anode with distilled water.
- Switch on the O2k and start DatLab.
- Record the signal for 10 min.
- Connect the SmartPOS to the O2k-main unit. Set an event in DatLab called "SmartPOS".
- Record the signal for 10 min.
- The leak current of each individual component should be stable and below 0.001 Β΅A (DatLab 8). If the SmartPOS is giving a high leak current a sensor service frequently solves the problem. If you have any uncertainties, please forward the file to the technical support.
O2k-series D-I and XA
- Unplug the POS-Connector from the O2k-main unit and unscrew the OroboPOS from the POS-Connector. Screw the POS to the blue part of the perspex housing.
- Remove the membrane from the POS and wash the cathode and anode with distilled water.
- Switch on the O2k and start DatLab.
- Record the signal for 10 min.
- Connect the POS-Connector to the O2k-main unit without the POS. Set an event in DatLab called "POS-Connector".
- Record the signal for 10 min.
- Screw the POS to the POS-Connector. Set an event in DatLab called "POS".
- Record the signal for 10 min.
- The leak current of each individual component should be stable and below 0.001 Β΅A (DatLab 8) or V (DatLab 7 and previous versions). Depending which component is giving a high leak current, a sensor service, followed by cleaning the electrical connections and drying the OroboPOS (without membrane) or the POS-Connector in an oven at up to 60 Β°C for 1-2 h frequently solves the problem. If you have any uncertainties, please forward the file to the technical support.
O2k-series A-C
- Plugging or unplugging of the POS-Connector while the main unit is switched on could damage the POS-Connector of O2k-series A-C. Therefore, the following should be started with the O2k switched off.
- Unplug the POS-Connector from the O2k-main unit and unscrew the OroboPOS from the POS-Connector. Screw the POS to the blue part of the perspex housing.
- Remove the membrane from the POS and wash the cathode and anode with distilled water.
- Plug the POS-Connector to the main unit.
- Switch on the O2k and start DatLab.
- Record the signal for 10 min.
- Screw the POS to the POS-Connector. Set an event in DatLab called "POS".
- Record the signal for 10 min.
- The leak current of each individual component should be stable and below 0.001 V (DatLab 7 and previous versions). Depending which component is giving a high leak current, a sensor service, followed by cleaning the electrical connections and drying the OroboPOS (without membrane) or the POS-Connector in an oven at up to 60 Β°C for 1-2 h frequently solves the problem. If you have any uncertainties, please forward the file to the technical support.
References
Example
- First, the OroboPOS-Connector and the POS were disassembled and disconnected from the main unit. Second, the OroboPOS-Connector was connected to the O2k-Main unit without the OroboPOS. Third, the OroboPOS was connected to the OroboPOS-Connector without membranes. After each step, the O2 raw signal [V] was recorded for 10 min. The marks were set to the O2 raw signal [V] to evaluate the leak current.
- Figure 1. Leak current test. Upper panel: Leak current is in the range of Β±0.00099 V. Lower panel: Leak current is higher than 0.00099 V when the OroboPOS-Connector is connected to the O2k-Main unit and when the OroboPOS is connected to the OroboPOS-Connector, indicating a potential electrical problem of both, the OroboPOS-Connector, and the OroboPOS.
Keywords
- Bioblast links: Oxygen signal - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
- Specific
- Β» Oxygen signal
- Β» O2k signal line
- Specific
- O2k-Procedures
- MiPNet O2k-Procedures
- General
- Β» Oxygen, dioxygen, O2
- Β» Oxygen calibration - DatLab
- Β» Oxygen solubility
- Β» Oxygen solubility factor
- Β» Oxygen pressure
- Β» Concentration
- Β» Activity
- Β» Pressure - Pascal
- Β» Barometric pressure
- Β» High-resolution respirometry
- Β» OroboPOS
- Β» Polarographic oxygen sensor
- Β» MitoFit Quality Control System
- Β» Sensitivity
- General
- Other keyword lists
MitoPedia O2k and high-resolution respirometry: O2k hardware, O2k-Open Support, O2k-Respirometry