Difference between revisions of "Ziak 2015 Mitochondrion"
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|preparations=Homogenate, Isolated mitochondria | |preparations=Homogenate, Isolated mitochondria | ||
|couplingstates=LEAK, OXPHOS, ETS | |couplingstates=LEAK, OXPHOS, ETS | ||
|substratestates=CI, CII | |substratestates=CI, CII, CI&II, ROX | ||
|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
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Revision as of 14:04, 7 November 2016
| Ziak J, Krajcova A, Jiroutkova K, Nemcova V, Dzupa V, Duska F (2015) Assessing the function of mitochondria in cytosolic context in human skeletal muscle: Adopting high-resolution respirometry to homogenate of needle biopsy tissue samples. Mitochondrion 21:106-12. |
Ziak J, Krajcova A, Jiroutkova K, Nemcova V, Dzupa V, Duska F (2015) Mitochondrion
Abstract: Using skeletal muscle homogenates for respirometry has many advantages, but the main challenge is avoiding the damage to outer mitochondrial membrane (OMM) and Complex I. By optimising the amount of muscle and careful titration of substrates and inhibitors we developed a new protocol and compared it to isolated mitochondria. We found acceptable damage to OMM (~ 10–15% increment of oxygen flux after addition of cytochrome c) and to Complex I (~ 70% of electron flux). Homogenate retained ~ 90% of phosphorylation capacity of isolated mitochondria. The use of fresh homogenate was crucial as mitochondrial function declined rapidly after 2–3 h of cold storage. • Keywords: High-resolution respirometry, Human skeletal muscle, Homogenate, Mitochondria, Needle biopsy
Labels: MiParea: Respiration, mt-Membrane
Organism: Human
Tissue;cell: Skeletal muscle
Preparation: Homogenate, Isolated mitochondria
Coupling state: LEAK, OXPHOS, ETS"ETS" is not in the list (LEAK, ROUTINE, OXPHOS, ET) of allowed values for the "Coupling states" property.
HRR: Oxygraph-2k
