Difference between revisions of "Template:SUIT-021"
From Bioblast
(Created page with "right|190px|link=http://www.bioblast.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT == Steps and respiratory states == [[File:#####.png|300px]] {| c...") |
|||
| Line 1: | Line 1: | ||
[[Image:SUIT-MitoFit.png|right|190px|link=http://www.bioblast.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]] | [[Image:SUIT-MitoFit.png|right|190px|link=http://www.bioblast.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]] | ||
== Steps and respiratory states == | == Steps and respiratory states == | ||
[[File: | [[File:1GM;2D;3U;4Ama.png|300px]] | ||
{| class="wikitable" border="1" | {| class="wikitable" border="1" | ||
Revision as of 15:09, 15 January 2019
Steps and respiratory states
| Step | State | Pathway | Q-junction | Comment - Events (E) and Marks (M) |
|---|---|---|---|---|
| 1GM | GML | N | CI | GML or GM_L: Glutamate & malate, N-LEAK respiration, NL
NADH-linked substrates (type N-pathway to Q). Template:SUIT L n |
| 2D | GMP | N | CI | 1GM;2D
NADH-linked substrates (type N-pathway to Q). OXPHOS capacity P (with saturating [ADP]), active OXPHOS state. |
| 3U | GME | N | CI | 1GM;2D;3U
NADH-linked substrates (type N-pathway to Q). Uncoupler titration (avoiding inhibition by high uncoupler concentrations) to obtain electron transfer (ET) capacity E (noncoupled ET-state). Test for limitation of OXPHOS capacity P by the phosphorylation system (ANT, ATP synthase, phosphate transporter) relative to ET capacity E in mt-preparations: E-P control efficiency and E-L coupling efficiency. In living cells: E-R control efficiency and E-L coupling efficiency. |
| 4Ama | ROX | Rox is the residual oxygen consumption in the ROX state, due to oxidative side reactions, estimated after addition of antimycin A (inhibitor of CIII). Rox is subtracted from oxygen flux as a baseline for all respiratory states, to obtain mitochondrial respiration (mt). |
