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Template:OSC Oxygen signal1

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Revision as of 16:22, 16 July 2019 by Gnaiger Erich (talk | contribs) (Created page with "=== Anonymous user === Question: Q1: The Oxygen Concentration couldn't go down while I tried zero oxygen calibration. No matter how much solution I added, the oxygen concent...")
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Anonymous user

Question:

Q1: The Oxygen Concentration couldn't go down while I tried zero oxygen calibration. No matter how much solution I added, the oxygen concentration still remain the same. I reassembled, but it still keeps the same. The data is attached.

No response to dithionite.PNG


Q2: The chamber B's oxygen slope is not stable. This is a new problem I have never seen. Please see the attachment.(2019-04-26)

Signal stability.PNG


Q3. The maximum Oxygen concentration of another Oroboros machine is only about 140. I don't how to increase the starting point of oxygen concentration.


Answer:

A1: I suspect that the dithionite solution was not correctly prepared or not fresh. Dithionite loses activity quite fast, so you need to make it freshly. Please check this site: http://bioblast.at/index.php/Dithionite

A2: While there is a difference between chambers A and B, the stability of the signal is still within the acceptable range of noise given by the specifications of the instrument, see: Oxygen_sensor_test.

A3: You may simply need to calibrate, please see here: http://wiki.oroboros.at/index.php/O2_calibration_-_DatLab