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Difference between revisions of "Talk:SUIT protocol library"

From Bioblast
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== Future Projects/Protocols ==
== Future Projects/Protocols ==


__TOC__


== Fish - Oxidative stress ==
== Fish - Oxidative stress ==
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* Protocol 2: PGM + '''D-titration + c + Omy''' + F-titration + Rot + Mna + Ama
* Protocol 2: PGM + '''D-titration + c + Omy''' + F-titration + Rot + Mna + Ama
* Protocol 3: PGM + '''D-titration + Omy + c''' + F-titration + Rot + Mna + Ama
* Protocol 3: PGM + '''D-titration + Omy + c''' + F-titration + Rot + Mna + Ama
== Fish - Oxidative stress ==
* Protocol 1: G + M + D + H202 (wait 20 min) + Ctl + c + P + D + S + Omy + FCCP+ Rot + Mna + Ama
* Protocol 2: G + M + D + H202 (wait 20 min) + Ctl + c + P + D + S * Rot + Omy + FCCP + + Rot + Mna + Ama
: see the following protocol for the H202 - oxidative stressΒ  - treatment: [[DSL_intern#Protocol:_2011-10-06]]
: '''Rational:''' calculation of P/E is not possible, but the effect of Omy is of interest

Revision as of 11:18, 10 October 2011

Future Projects/Protocols

Fish - Oxidative stress

  • Protocol 1: G + M + D + H202 (wait 20 min) + Ctl + c + P + D + S + Omy + FCCP+ Rot + Mna + Ama
  • Protocol 2: G + M + D + H202 (wait 20 min) + Ctl + c + P + D + S * Rot + Omy + FCCP + + Rot + Mna + Ama
see the following protocol for the H202 - oxidative stress - treatment: DSL_intern#Protocol:_2011-10-06
Rational: calculation of P/E is not possible, but the effect of Omy is of interest


Mouse brain - Shredder

  • Idea by Erich, 2011-10-07
  • Method: Shredder
  • Rational: test these different protocols to see the effect of cytochrome c and Oligomycin at different positions in the protocol


  • Protocol 1: PGM + c + D-titration + Omy + F-titration + Rot + Mna + Ama
  • Protocol 2: PGM + D-titration + c + Omy + F-titration + Rot + Mna + Ama
  • Protocol 3: PGM + D-titration + Omy + c + F-titration + Rot + Mna + Ama