Talk:SUIT-006 MgG mt D055
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Revision as of 17:26, 2 September 2020 by Doerrier Carolina (talk | contribs)
Step | Chemical(s) and link(s) | Comments |
---|---|---|
1PM | Pyruvate (P) and Malate (M) | Can be substituted by other combination of substrates |
2D | ADP (D) preparation for MgG assay | For this protocol, the ADP must be prepared without Mg2+ salts. |
3Cat | Carboxyatractyloside (Cat) | Can be substituted for oligomycin |
4U | SF6847 | Can be substituted for other uncoupler |
5Ama | Antimycin A (Ama) | Can be substituted for other CIII or CIV inhibitor |
Chemical and link | Abbreviation | Chemical and link |
---|---|---|
Magnesium Green | MgG | |
MgCl2 | Mg |
Chemical and link | Abbreviation | Comments |
---|---|---|
Pyruvate | P | Can be substituted by other combination of substrates |
Malate | M | Can be substituted by other combination of substrates |
ADP | D | For this protocol, the ADP must be prepared without Mg2+ salts. |
Carboxyatractyloside | Cat | Can be substituted for oligomycin |
Uncoupler: in this DLP, SF6847 was used | U | Can be substituted for other uncoupler |
Antimycin A | Ama | Can be substituted for other CIII or CIV inhibitor |