Difference between revisions of "Talk:Oxygen calibration - DatLab"

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Talk:Oxygen calibration - DatLab

MitoPedia O2k and high-resolution respirometry: O2k-Open Support

Description

O2 calibration is the calibration in DatLab of the oxygen sensor. It is a prerequisite for obtaining accurate measurements of respiration. Accurate calibration of the oxygen sensor depends on (1) equilibration of the incubation medium with air oxygen partial pressure at the temperature defined by the experimenter; (2) zero oxygen calibration; (3) high stability of the POS signal tested for sufficiently long periods of time; (4) linearity of signal output with oxygen pressure in the range between oxygen saturation and zero oxygen pressure; and (5) accurate oxygen solubility for aqueous solutions for the conversion of partial oxygen pressure into oxygen concentration. The standard oxygen calibration procedure is described below for high-resolution respirometry with the calibration routine using instrumental calibration DL-Protocols in DatLab.

Reference: MiPNet06.03 POS-calibration-SOP, MiPNet12.20_O2k-calibration_tutorial

Oxygen calibration - when?

Air calibration / Quality control

Daily air calibration goes hand in hand with a stirrer test and inspection of the recorded data in DatLab, also called oxygen sensor test. The procedure is decisive for maintaining a high level of O2k quality control as described in QC 1: Oxygen sensor test.

Zero oxygen calibration: less frequent

Indicator fields: As a reminder, the calibration indicator fields in the bottom right and bottom left corner of the experiment window are colored yellow after starting an experiment. After selecting Calibration > Open or pressing F5 and confirming the calibration, the field changes to green (in DatLab 8). How do they behave in DatLab 7?

Generate R1 and R0

record the oxygen signal while the chamber is open
after equilibration set a mark and call it R1

or perform steps 1. and 2. guided by the air calibration protocol

close the chamber, remove the oxygen from the medium
set a mark and call it R0

or perform steps 3. and 4. guided by the air and zero calibration protocol

DatLab 8

How to get R1 and R0
calibration values are stored together with instrument identifiers and sensor numbers
bottom left and bottom right: yellow until calibration has been confirmed

DatLab 7.4

see current content of the page

Details

marks: what is it, how to set it, how to extract values
events: what is it, how to set it
special situation: one computer, several O2ks connected

@@ Line 11: / Line 11: @@
 __TOC__
-== General Information ==
+== Oxygen calibration - when? ==
-* why do we need calibration
+; Air calibration / Quality control
-* how often + link to quality control [[Oxygen_sensor_text]]: add comments about frequency there
-* what do we do with the instrument
+: Daily air calibration goes hand in hand with a stirrer test and inspection of the recorded data in DatLab, also called [[Oxygen_sensor_test|oxygen sensor test]]. The procedure is decisive for maintaining a high level of O2k quality control as described in [[O2k-Open_Support#O2k-Open Support: technical help#1. O2 sensor test| QC 1: Oxygen sensor test]].
-* using a protocol. With or without the protocol: we need marks R1 and R0 to proceed.
+; Zero oxygen calibration
+: less frequent
+; Indicator fields
+: As a reminder, the calibration indicator fields in the bottom right and bottom left corner of the experiment window are colored yellow after starting an experiment. After selecting Calibration > Open or pressing F5 and confirming the calibration, the field changes to green (in DatLab 8). How do they behave in DatLab 7?
+; Generate R1 and R0
+# record the oxygen signal while the chamber is open
+# after equilibration set a mark and call it R1
+::* or perform steps 1. and 2. guided by the air calibration protocol
+# close the chamber, remove the oxygen from the medium
+# set a mark and call it R0
+::* or perform steps 3. and 4. guided by the air and zero calibration protocol
 == DatLab 8 ==