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Difference between revisions of "Talk:NextGen-O2k Scientific developments"

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== Track record ==
== Track record ==
:::: '''2021-06-30:''' [[Bioenerg Commun]] publication: [[Cardoso_2021_BEC_MgG|"Magnesium Green for fluorometric measurement of ATP production does not interfere with mitochondrial respiration"]]. Β 
:::: '''2021-11-11:''' [[Bioenerg Commun]] publication: [[Komlodi 2021 MitoFit Q|"Coupling and pathway control of coenzyme Q redox state and respiration in isolated mitochondria"]]
:::: '''2021-11-08:''' MitoFit preprint: [[Komlodi 2021 MitoFit AmR-O2|"Facts and artefacts on the oxygen dependence of hydrogen peroxide production using Amplex UltraRed"]]
:::: '''2021-10-28:''' Deliverable 2.2 submitted.
:::: '''2021-09-21:''' MitoFit preprint: [[Gnaiger 2021 MitoFit BCA|"Bioenergetic cluster analysis – mitochondrial respiratory control in human fibroblasts"]]
:::: '''2021-09-15:''' New version of the MitoFit preprint:[[Komlodi 2021 MitoFit Q|"Coupling and pathway control of coenzyme Q redox state and respiration in isolated mitochondria"]]
:::: '''2021-09-06:''' New version of the MitoFit preprint: [[Gnaiger 2020 MitoFit x|"The elementary unit β€” canonical reviewer's comments on: Bureau International des Poids et Mesures (2019) The International System of Units (SI) 9th ed"]]
:::: '''2021-09-01:''' New version of the MitoFit preprint: [[Komlodi 2021 MitoFit Q|"Coupling and pathway control of coenzyme Q redox state and respiration in isolated mitochondria"]]
:::: '''2021-06-30:''' [[Bioenerg Commun]] publication: [[Cardoso_2021_BEC_MgG|"Magnesium Green for fluorometric measurement of ATP production does not interfere with mitochondrial respiration"]].
:::: '''2021-06-09:''' "Coenzyme Q redox state and high-resolution respirometry: Novel perspectives for mitochondrial diagnostics" was presented at [[ESCI 2021 Virtual]]
:::: '''2021-06-09:''' "Coenzyme Q redox state and high-resolution respirometry: Novel perspectives for mitochondrial diagnostics" was presented at [[ESCI 2021 Virtual]]
:::: '''2021-05-20:''' The poster "Oxygen dependence of photosynthesis and light-enhanced dark respiration studied by High-Resolution PhotoRespirometry" was presented at [[ISAP 2021 Virtual]]
:::: '''2021-05-20:''' The poster "Oxygen dependence of photosynthesis and light-enhanced dark respiration studied by High-Resolution PhotoRespirometry" was presented at [[ISAP 2021 Virtual]]
:::: '''2021-05-06:''' New version of the MitoFit preprint: [[Komlodi 2021 MitoFit Q|"Coupling and pathway control of coenzyme Q redox state and respiration in isolated mitochondria"]].
:::: '''2021-04-30:''' MitoFit preprint on the PB-Module: [[Went 2021 MitoFit PB|Oxygen dependence of photosynthesis and light-enhanced dark respiration studied by High-Resolution PhotoRespirometry]]
:::: '''2021-04-30:''' MitoFit preprint on the PB-Module: [[Went 2021 MitoFit PB|Oxygen dependence of photosynthesis and light-enhanced dark respiration studied by High-Resolution PhotoRespirometry]]
:::: '''2021-03-09:''' Start of biological experiments to optimize experimental conditions for measuring mitochondrial respiration and NADH autofluorescence with the NextGen-O2k. Β 
:::: '''2021-03-09:''' Start of biological experiments to optimize experimental conditions for measuring mitochondrial respiration and NADH autofluorescence with the NextGen-O2k. Β 

Revision as of 08:37, 20 December 2021

Track record

2021-11-11: Bioenerg Commun publication: "Coupling and pathway control of coenzyme Q redox state and respiration in isolated mitochondria"
2021-11-08: MitoFit preprint: "Facts and artefacts on the oxygen dependence of hydrogen peroxide production using Amplex UltraRed"
2021-10-28: Deliverable 2.2 submitted.
2021-09-21: MitoFit preprint: "Bioenergetic cluster analysis – mitochondrial respiratory control in human fibroblasts"
2021-09-15: New version of the MitoFit preprint:"Coupling and pathway control of coenzyme Q redox state and respiration in isolated mitochondria"
2021-09-06: New version of the MitoFit preprint: "The elementary unit β€” canonical reviewer's comments on: Bureau International des Poids et Mesures (2019) The International System of Units (SI) 9th ed"
2021-09-01: New version of the MitoFit preprint: "Coupling and pathway control of coenzyme Q redox state and respiration in isolated mitochondria"
2021-06-30: Bioenerg Commun publication: "Magnesium Green for fluorometric measurement of ATP production does not interfere with mitochondrial respiration".
2021-06-09: "Coenzyme Q redox state and high-resolution respirometry: Novel perspectives for mitochondrial diagnostics" was presented at ESCI 2021 Virtual
2021-05-20: The poster "Oxygen dependence of photosynthesis and light-enhanced dark respiration studied by High-Resolution PhotoRespirometry" was presented at ISAP 2021 Virtual
2021-05-06: New version of the MitoFit preprint: "Coupling and pathway control of coenzyme Q redox state and respiration in isolated mitochondria".
2021-04-30: MitoFit preprint on the PB-Module: Oxygen dependence of photosynthesis and light-enhanced dark respiration studied by High-Resolution PhotoRespirometry
2021-03-09: Start of biological experiments to optimize experimental conditions for measuring mitochondrial respiration and NADH autofluorescence with the NextGen-O2k.
2021-02-18: MitoFit preprint publication: Coupling and pathway control of coenzyme Q redox state and respiration in isolated mitochondria
2021-02-09: Start of the last biological replicates measuring oxygen kinetics of the contribution to total dark respiration of the AOX-Q branch in Chlamydomonas reinhardtii.
2021-01-29: Deliverable 2.1 submitted.
2021-01-29: MitoFit preprint publication: Magnesium Green for fluorometric measurement of ATP production does not interfere with mitochondrial respiration
2020-12-04: Start of the development of a white PB-Sensor for the PB-Module in collaboration with the algae KOLs
2020-11-30: The poster showing the latest scientific developments on the PB-Module, presented on the AlgaEurope 2020, was published in MitoFit Preprints: High-resolution respirometry for chloroplast and mitochondrial bioenergetics in Chlamydomonas reinhardtii ― towards biotechnology exploitations
2020-11-06: SUIT protocols (SUIT-030 family) to determine the maximal photosynthesis rate under light- and inorganic carbon-saturating conditions optimized for microalgal living cells.
2020-10-27: First tests of the NADH-Module integrated in the NextGen-O2k.
2020-10-22: Optimization of the optimal conditions for measuring photosynthesis in the marine microalgae Phaeodactylum tricornutum with the PB-Module.
2020-10-08: Laurent Beck started with measurements on Chlamydomonas reinhardtii using the PB- and Q-Modules , supervised by PI Maria Huete-Ortega and Thomas Roach (University of Innsbruck, Institute of Botany)
2020-07-23: First isolation of mitochondria in microalgae trials
2020-07-01: Start of the development of protocols for permeabilizing microalgae cells
2020-04-24: First tests with pH electrodes
2020-03-11: First tests with Calcium Green
2020-02-20: Started development of protocols (and DL-Protocols) for the PB-Module, including new SUIT protocols for photosynthesis measurements.
2019-12-31: Determination of optimal Q-sensor measurement conditions for different biological samples (brain and heart isolated mitochondria from mouse, yeast)
2019-12-06: First version of the Cyclic Voltammetry Manual released: CV-Module
2019-12-02: Optimization of O2k-Procedures for isolated mitochondria preparations
2019-11-22: First version of NextGen-O2k Manual/SOP finalized: NextGen-O2k: Q-Module
2019-11-21: Updated beta version of cyclic voltammetry provided to the Key Opinion Leaders for testing
2019-11-18: Application of Q-redox state measurements in scientific projects
2019-10-22: 66 SUIT protocols for different O2k-Applications (i.e. oxygen consumption, hydrogen peroxide production, mitochondrial membrane potential, ATP production) which can be measured in living cells, permeabilized cells, isolated mitochondria, tissue homogenate and permeabilized fibers optimized and released with DatLab 7.4, see MitoPedia:_SUIT
2019-10-21: Four new videos for DatLab 7.4 software released - see: O2k-Videosupport DatLab 7.4
2019-09-24: Two new videos for mitochondrial preparations released - see: O2k-Videosupport Mitochondrial preparations
2019-09-23: First beta version of the cyclic voltammetry used by the Key Opinion Leaders during 1st Q-Module workshop at the Oroboros O2k-Workshop IOC141
2019-09-20: First six videos for the Q-Module released - see: O2k-Videosupport Q-Module
2019-09-06: Development of two harmonized SUIT protocols (and DL-Protocols) for the Q-Module, both provided to the Key Opinion Leaders
2019-07-17: First tests of the new O2 analysis template DL7.4
2019-07: Started development of SUIT protocols for isolated mitochondria and permeabilzied cells
2019-06-18: Testing of the SUITbrowser by the participants at the Oroboros O2k-Workshop on high-resolution respirometry (HRR), IOC139
2019-06: Integration of cyclic voltammetry as a quality control step into the Q-Module
2019-06: Testing of the SUITbrowser