Difference between revisions of "Succinate"
From Bioblast
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{{MitoPedia | {{MitoPedia | ||
|abbr=S | |abbr=S | ||
|description='''Succinate<sup>2-</sup>''' is formed in the [[TCA cycle]], and is a substrate of [[CII]], reacting to fumarate and feeding electrons into the [[Q-junction]]. Succinate (CII-linked) and NADH (CI-linked) provide convergent electron entries into the Q-junction. | |description='''Succinate<sup>2-</sup>''' is formed in the [[TCA cycle]], and is a substrate of [[CII]], reacting to fumarate and feeding electrons into the [[Q-junction]]. Succinate (CII-linked) and NADH (CI-linked) provide convergent electron entries into the Q-junction. Succinate is transported across the inner mitochondrial membrane by the [[dicarboxylate carrier]]. | ||
|info=[http://www.oroboros.at/?Gnaiger_2012_MitoPathways Gnaiger 2012 MitoPathways | |info=[http://www.oroboros.at/?Gnaiger_2012_MitoPathways Gnaiger 2012 MitoPathways] | ||
|type=Respiration | |type=Respiration | ||
}} | }} | ||
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'''Preparation of 1 M stock solution''' (dissolved in H<sub>2</sub>O): | '''Preparation of 1 M stock solution''' (dissolved in H<sub>2</sub>O): | ||
::1) | ::1) Weigh 1.3505 g of succinate and dissolve in 3 ml H<sub>2</sub>O. | ||
::2) | ::2) Check pH and adjust to 7.0 if necessary with 1 N HCl (usually the pH is 7 without any adjustment). | ||
::3) | ::3) Transfer to 5 ml volumetric glass flask and adjust the final volume to 5 ml. | ||
::4) | ::4) Divide into 0.2 ml portions. | ||
::5) | ::5) Store frozen at -20 °C. | ||
'''Oxygraph-2k Manual Titrations:''' [[MiPNet09.12]] | '''Oxygraph-2k Manual Titrations:''' [[MiPNet09.12]] | ||
::* In the absence of CI-linked substrates, add rotenone before addition of succinate, to avoid accumulation of oxaloacetate with subsequent | ::* In the absence of CI-linked substrates, add the CI-inhibitor [[rotenone]] before addition of succinate, to avoid accumulation of [[oxaloacetate]] with subsequent inhibition of succiante dehydrogenase. | ||
::* Titration volume: 20 µl using a 50 µl syringe (2 ml O2k-chamber) | ::* When keeping the succinate stock solution on ice, check for complete solubilization of succinate and warm in your hands if necessary. | ||
::* Final concentration: 10 mM | ::* Titration volume: 20 µl using a 50 µl syringe (2 ml O2k-chamber). | ||
::* Final concentration: 10 mM. |
Revision as of 20:41, 1 June 2013
Description
Succinate2- is formed in the TCA cycle, and is a substrate of CII, reacting to fumarate and feeding electrons into the Q-junction. Succinate (CII-linked) and NADH (CI-linked) provide convergent electron entries into the Q-junction. Succinate is transported across the inner mitochondrial membrane by the dicarboxylate carrier.
Abbreviation: S
Reference: Gnaiger 2012 MitoPathways
MitoPedia topics: Substrate and metabolite
Application in HRR
S: Succinate (Succinate disodium salt, hexahydrate, C4H404Na2 * (H2O)6); Sigma S 2378, 100 g, store at R.T.; FW = 270.1
Preparation of 1 M stock solution (dissolved in H2O):
- 1) Weigh 1.3505 g of succinate and dissolve in 3 ml H2O.
- 2) Check pH and adjust to 7.0 if necessary with 1 N HCl (usually the pH is 7 without any adjustment).
- 3) Transfer to 5 ml volumetric glass flask and adjust the final volume to 5 ml.
- 4) Divide into 0.2 ml portions.
- 5) Store frozen at -20 °C.
Oxygraph-2k Manual Titrations: MiPNet09.12
- In the absence of CI-linked substrates, add the CI-inhibitor rotenone before addition of succinate, to avoid accumulation of oxaloacetate with subsequent inhibition of succiante dehydrogenase.
- When keeping the succinate stock solution on ice, check for complete solubilization of succinate and warm in your hands if necessary.
- Titration volume: 20 µl using a 50 µl syringe (2 ml O2k-chamber).
- Final concentration: 10 mM.