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- Barison 2016 J Bioenerg Biomembr + (''Trypanosoma cruzi'', the aetiological ag … ''Trypanosoma cruzi'', the aetiological agent of Chagas's disease, metabolizes glucose, and after its exhaustion, degrades amino acids as energy source. Here, we investigate histidine uptake and its participation in energy metabolism. No putative genes for the histidine biosynthetic pathway have been identified in genome databases of ''T. cruzi'', suggesting that its uptake from extracellular medium is a requirement for the viability of the parasite. From this assumption, we characterized the uptake of histidine in ''T. cruzi'', showing that this amino acid is incorporated through a single and saturable active system. We also show that histidine can be completely oxidised to CO<sub>2</sub>. This finding, together with the fact that genes encoding the putative enzymes for the histidine - glutamate degradation pathway were annotated, led us to infer its participation in the energy metabolism of the parasite. Here, we show that His is capable of restoring cell viability after long-term starvation. We confirm that as an energy source, His provides electrons to the electron transport chain, maintaining mitochondrial inner membrane potential and O<sub>2</sub> consumption in a very efficient manner. Additionally, ATP biosynthesis from oxidative phosphorylation was found when His was the only oxidisable metabolite present, showing that this amino acid is involved in bioenergetics and parasite persistence within its invertebrate host.oenergetics and parasite persistence within its invertebrate host.)
