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• Cytochrome c
  ...cy]]). The following DatLab traces illustrate examples of cytochrome ''c'' addition: ...flux per volume (red trace, right axis) can be seen upon cytochrome ''c'' addition ([[Cytochrome c control efficiency|cytochrome ''c'' control efficiency]]=0.
  6 KB (844 words) - 09:37, 4 April 2024
• SUIT-031 Q mt D072
  ...rotenone does not have a further effect on the Q-signal in the presence of sample and CoQ₂, this test can be omitted. ...or isolated mitochondria, tissue homogenate and cells permeabilized before addition to the O2k- chamber.
  4 KB (530 words) - 16:17, 13 December 2023
• SUIT-031 Q ce-pce D074
  ...rotenone does not have a further effect on the Q signal in the presence of sample and [[coenzyme Q2]], this test can be omitted. ...or isolated mitochondria, tissue homogenate and cells permeabilized before addition to the O2k- chamber.
  4 KB (519 words) - 17:52, 13 December 2023
• Schoepf 2014 Abstract MiP2014
  ...ial response to cellular stress exerted by enhanced ROS production [4]. In addition, an easy and fast assay for determination of CIV activity was applied addin ...d in MiR05 with creatine (MiR05Cr) at 37 °C. Parallel analyses of a paired sample consisting of one benign and one malign biopsy from a single patient could
  7 KB (962 words) - 18:31, 10 January 2022
• Setting the oxygen concentration
  ...or decreasing oxygen concentration during an experiment with a biological sample is relevant for specific applications (''e.g.'', hypoxia, tissue normoxia, ...rted to O₂ with a high catalase concentration able to avoid any sample oxidative stress. During this procedure the O2k-chamber remains closed, dec
  5 KB (828 words) - 17:10, 31 January 2024
• Cytochrome c control efficiency
  .... https://doi.org/10.26124/bec:2020-0002</ref>, ''j''_{cyt ''c''}. Addition of [[cytochrome c| '''cytochrome ''c''''']] (10 µM final concentration)<re ...wo values to calculate the increase of respiration due to cytochrome ''c'' addition.
  11 KB (1,496 words) - 11:35, 21 January 2022
• SUIT-007
  .... If [[Glutamate dehydrogenase|glutamate dehydrogenase]] is present in the sample, when glutamate alone is added, it will be converted to α-ketoglutarate in ...the mitochondrial external membrane as it would be with GM. However, with addition of cytochrome ''c'' only after malate (e.g. 1G;2D;3M;3c), it would not be p
  3 KB (401 words) - 11:30, 3 June 2020
• Kane 2016 Abstract MitoFit Science Camp 2016
  ...ore, be to introduce contraction inhibitor early in the preparation of the sample. ...ated with myosin II ATPase inhibitor blebbistatin (bleb) during the entire sample preparation exhibit different JO2 when assayed in standard MiR06 sucrose re
  6 KB (790 words) - 17:46, 7 November 2016
• Steady state
  ..., since the increase in the oxygen flux is not due to the cytochrome ''c'' addition, but instead because flux increases further over time (see Figure 2 for mor ...ed by the straight line (from 2D to 2c) is not due to the cytochrome ''c'' addition. More time would have been requred to reach a stable flux before step 2c. C
  8 KB (1,115 words) - 03:00, 23 January 2023
• O2k-Videosupport
  ...j5GfuUwDI ]] [https://www.youtube.com/watch?v=NJj5GfuUwDI Insertion of the Sample Holder into the 2.0 mL O2k-chamber] ...Xgg ]] [https://www.youtube.com/watch?v=GX_SvnVGXgg Addition of biological sample to the respirometer chamber]
  9 KB (1,435 words) - 12:42, 14 November 2023
• SUIT-006 02 mt D108
  ...ed after the experiment to avoid carry-over of inhibitors. The addition of sample homogenate is recommended in the washing protocol to bind strong inhibitors
  2 KB (233 words) - 10:43, 26 January 2024
• Krajcova 2020 PLOS ONE
  ...d integrity of outer mitochondrial membrane (increase of respiration after addition of cytochrome ''c'' is up to 11.7±1.8 % vs. 15.7±3.1 %, ''p''˂0.05 and 1
  3 KB (373 words) - 03:31, 18 April 2024
• SUIT-034 O2 mt D111
  :::- When ATPases are present in the sample, it is not possible to reach the [[LEAK]] state. ...after the experiment to avoid carry-over of uncoupler and inhibitors. The addition of liver homogenate is recommended in the washing protocol to bind strong i
  2 KB (244 words) - 09:56, 26 January 2024
• SUIT-026 AmR ce-pce D087
  ...D022]] for quality control and a full assessment of the coupling control. Addition of [[Antimycin A|Ama]] leads to increased ROS production via inhibition of ...nd_data_analysis|mt outer membrane integrity]] as a quality control of the sample).
  4 KB (562 words) - 13:52, 31 August 2023
• SUIT-026 AmR mt D077
  ...D022]] for quality control and a full assessment of the coupling control. Addition of [[Antimycin A|Ama]] leads to increased ROS production via inhibition of ...nd_data_analysis|mt outer membrane integrity]] as a quality control of the sample).
  4 KB (575 words) - 12:06, 31 August 2023
• SUIT-033 O2 mt D110
  :::- When ATPases are present in the sample, it is not possible to reach the [[LEAK]] state. ...ng is required after the experiment to avoid carry-over of inhibitors. The addition of liver homogenate is recommended in the washing protocol to bind strong i
  2 KB (238 words) - 09:57, 26 January 2024
• Jiroutkova 2014 Intensive Care Medicine Experimental
  ...ely. Integrity of outer membrane was assessed by measuring the response to addition of cytochrome c. Oxygen consumption rate was normalized to citrate-synthase
  3 KB (402 words) - 12:09, 9 December 2021
• SUIT-026 AmR mt D064
  ...D022]] for quality control and a full assessment of the coupling control. Addition of [[Antimycin A|Ama]] leads to increased ROS production via inhibition of ...nd_data_analysis|mt outer membrane integrity]] as a quality control of the sample).
  4 KB (609 words) - 00:00, 13 April 2021
• Stelfa 2018 MiP2018
  ...viously [1]. To induce anoxia, respiration of sample was stimulated by the addition of succinate with rotenone and ADP and preparation was left to consume all ...sub>2</sub>O₂/O flux ratio after anoxia-reoxygenation. Overall, addition of R-phenibut prevented anoxia-reoxygenation-induced increase in H<sub>2</s
  4 KB (521 words) - 12:48, 17 March 2019
• MitoEAGLE protocols, terminology, documentation
  ...al for large-scale production. For respiratory OXPHOS analysis a reference sample of structurally and functionally intact mitochondria is not yet available t ...ides the ability to make diagnostic profiling associated with diseases. In addition to functional data, other data types such as nutritional or environmental f
  11 KB (1,507 words) - 12:05, 19 January 2018

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