Sharma 2023 Biosci Biotechnol Biochem

Sharma S, Zhang X, Azhar G, Patyal P, Verma A, Kc G, Wei JY (2023) Valine improves mitochondrial function and protects against oxidative stress. https://doi.org/10.1093/bbb/zbad169

» Biosci Biotechnol Biochem [Epub ahead of print]. PMID: 38093456 Open Access

Sharma Shakshi, Zhang Xiaomin, Azhar Gohar, Patyal Pankaj, Verma Ambika, Kc Grishma, Wei Jeanne Y (2023) Biosci Biotechnol Biochem

Abstract: Among the branched chain amino acids (BCAAs), leucine and isoleucine have been well-studied for their roles in improving mitochondrial function and reducing oxidative stress. However, role of valine in mitochondrial function regulation and oxidative stress management remains elusive. This study investigated valine effect on mitochondrial function and oxidative stress in vitro. Valine increased expression of genes involved in mitochondrial biogenesis and dynamics. It upregulates mitochondrial function at Complexes I, II and IV levels of electron transport chain. Flow cytometry studies revealed, valine reduced oxidative stress by significantly lowering mitochondrial reactive oxygen species (ROS) and protein expression of 4 hydroxynonenal. Functional role of valine against oxidative stress was analyzed by XFe96 Analyzer. Valine sustained oxidative phosphorylation and improved ATP generation rates during oxidative stress. In conclusion, our findings shed more light on the critical function of valine in protecting mitochondrial function thereby preventing mitochondrial/cellular damage induced by oxidative stress. • Keywords: Mitochondrial function, Oxidative stress, Reactive oxygen species, Valine • Bioblast editor: Plangger M

Labels: MiParea: Respiration, Exercise physiology;nutrition;life style, Pharmacology;toxicology 

Stress:Oxidative stress;RONS  Organism: Mouse  Tissue;cell: Skeletal muscle  Preparation: Permeabilized cells, Intact cells 

Coupling state: LEAK, ROUTINE, OXPHOS, ET  Pathway: N, S, CIV, ROX  HRR: Oxygraph-2k 

2023-12