Difference between revisions of "Schaefer 2016 Abstract ADFLIM 2016"
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{{Abstract | {{Abstract | ||
|title=ADFLIM in AD research –imaging mitochondrial function in Alzheimer´s disease | |title=ADFLIM in AD research –imaging mitochondrial function in Alzheimer´s disease | ||
|authors=Schaefer PM, Einem B | |info=[[File:SchaeferP.jpg|120px|right|Patrick Schaefer]] | ||
|authors=Schaefer PM, von Einem B, Niederschweiberer M, Kalinina S, Walther P, Calzia E, Rueck A, von Arnim CAF | |||
|year=2016 | |year=2016 | ||
|event=ADFLIM 2016 | |event=ADFLIM 2016 | ||
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To further elucidate the role of the intracellular localization of both proteins in mitochondrial impairment, we performed metabolic characterizations of intact cells overexpressing the respective proteins. Using high-resolution respirometry and electron microscopy, we demonstrate especially the intracellular/mitochondrial pool of Aβ to lower mitochondrial respiration. | To further elucidate the role of the intracellular localization of both proteins in mitochondrial impairment, we performed metabolic characterizations of intact cells overexpressing the respective proteins. Using high-resolution respirometry and electron microscopy, we demonstrate especially the intracellular/mitochondrial pool of Aβ to lower mitochondrial respiration. | ||
As the toxic potential of intracellular Aβ underlines the rational of a selective vulnerability of different cell types to Aβ-induced mitochondrial defects, we established a multimodal optical system to measure cell metabolism on the single cell level. Relying on NADH fluorescence lifetime imaging microscopy (NADH FLIM), here we demonstrate that our optical metabolic imaging system is able to mirror the results obtained in the | As the toxic potential of intracellular Aβ underlines the rational of a selective vulnerability of different cell types to Aβ-induced mitochondrial defects, we established a multimodal optical system to measure cell metabolism on the single cell level. Relying on NADH fluorescence lifetime imaging microscopy (NADH FLIM), here we demonstrate that our optical metabolic imaging system is able to mirror the results obtained in the Oroboros Oxygraph-2k and in surplus displays subcellular resolution representing mitochondrial and neuronal heterogeneity in AD. | ||
Our results demonstrate the importance of assessing energy metabolism on the single cell level to shed light onto Alzheimer´s disease associated mitochondrial dysfunction, highlighting the potential of NADH FLIM for metabolic characterization. | Our results demonstrate the importance of assessing energy metabolism on the single cell level to shed light onto Alzheimer´s disease associated mitochondrial dysfunction, highlighting the potential of NADH FLIM for metabolic characterization. | ||
|mipnetlab=DE Ulm Radermacher P | |||
}} | }} | ||
{{Labeling | {{Labeling | ||
Latest revision as of 15:38, 23 January 2019
| ADFLIM in AD research –imaging mitochondrial function in Alzheimer´s disease |
Link:
Schaefer PM, von Einem B, Niederschweiberer M, Kalinina S, Walther P, Calzia E, Rueck A, von Arnim CAF (2016)
Event: ADFLIM 2016
Mitochondrial dysfunction is known as an early feature of Alzheimer´s disease (AD). Amyloid beta (Aβ) as well as its precursor protein APP were identified as key players provoking these mitochondrial disturbances. This entails an energy imbalance in the brain, being one trigger of neuronal death in Alzheimer´s disease.
To further elucidate the role of the intracellular localization of both proteins in mitochondrial impairment, we performed metabolic characterizations of intact cells overexpressing the respective proteins. Using high-resolution respirometry and electron microscopy, we demonstrate especially the intracellular/mitochondrial pool of Aβ to lower mitochondrial respiration. As the toxic potential of intracellular Aβ underlines the rational of a selective vulnerability of different cell types to Aβ-induced mitochondrial defects, we established a multimodal optical system to measure cell metabolism on the single cell level. Relying on NADH fluorescence lifetime imaging microscopy (NADH FLIM), here we demonstrate that our optical metabolic imaging system is able to mirror the results obtained in the Oroboros Oxygraph-2k and in surplus displays subcellular resolution representing mitochondrial and neuronal heterogeneity in AD.
Our results demonstrate the importance of assessing energy metabolism on the single cell level to shed light onto Alzheimer´s disease associated mitochondrial dysfunction, highlighting the potential of NADH FLIM for metabolic characterization.
• O2k-Network Lab: DE Ulm Radermacher P
Labels: MiParea: Respiration Pathology: Alzheimer's Stress:Mitochondrial disease
Tissue;cell: Nervous system Preparation: Intact cells
HRR: Oxygraph-2k
Affiliations
1-Inst Neurology, 2-Central Facility Electron Microscopy, 3-Inst Anästhesiolog Pathophysiol Verfahrensentwicklung, 4-Core Facility Confocal Multiphoton Microscopy; Ulm University, Germany. - [email protected]
