- high-resolution terminology - matching measurements at high-resolution
Saponin
Description
Saponin (synonym: Sapogenin Glycosides1) is a mild detergent that permeabilizes plasma membranes completely and selectively due to their high cholesterol content, whereas mt-membranes with lower cholesterol content are affected only at higher concentrations. Applied for permeabilization of muscle fibres.
Abbreviation: Sap
MitoPedia topics:
Permeabilization agent
Application in HRR
Sap: Saponin (from quillaja bark); Sigma S 7900, 25 g, store at R.T.
Comment: Saponin from Quillaja Bark has the product numbers S 7900 and S 2149!
Caution: Prepare fresh everyday!
Preparation of 5 mg/ml stock solution (dissolved in H2O):
- 1) weigh 5 mg of Saponin
- 2) dissolve in 1 ml H2O
→ use 50 µg Saponin/ml BIOPS: add 20 µl of 5 mg/ml saponin stock solution per 2 ml BIOPS
