Difference between revisions of "Saponin"
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|abbr=Sap | |||
|description='''Saponin''' is a mild detergent that [[Permeabilization of plasma membrane|permeabilizes plasma membranes]] completely and selectively due to their high cholesterol content, whereas mt-membranes with lower cholesterol content are affected only at higher concentrations. Applied for [[Permeabilized tissue or cells|permeabilization of muscle fibres]]. | |description='''Saponin''' is a mild detergent that [[Permeabilization of plasma membrane|permeabilizes plasma membranes]] completely and selectively due to their high cholesterol content, whereas mt-membranes with lower cholesterol content are affected only at higher concentrations. Applied for [[Permeabilized tissue or cells|permeabilization of muscle fibres]]. | ||
| | |info=[[Doerrier 2018 Methods Mol Biol]] | ||
}} | }} | ||
{{MitoPedia concepts}} | |||
{{MitoPedia methods}} | |||
{{MitoPedia O2k and high-resolution respirometry}} | |||
{{MitoPedia topics | {{MitoPedia topics | ||
|mitopedia topic= | |mitopedia topic=Permeabilization agent | ||
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== Application in [[HRR]] == | |||
'''Sap: Saponin''' (from quillaja bark); Sigma S7900, 25 g, store at RT. | |||
'''Comment:''' ''Saponin from Quillaja Bark has the product numbers S7900 and S2149, but S2149 is withdrawn from Sigma''. | |||
<span style="color:#8B008B"> '''Caution:''' Prepare fresh everyday!</span> | |||
'''Preparation of 5 mg/mL stock solution (dissolved in''' [[Biopsy preservation solution|BIOPS]]): | |||
::1) Weigh 5 mg of saponin. | |||
::2) Dissolve in 1 mL BIOPS. | |||
→ Use 50 µg saponin/mL [[Biopsy preservation solution|BIOPS]]: add 20 µL of 5 mg/mL saponin stock solution per 2 mL BIOPS. | |||
Latest revision as of 15:42, 4 April 2024
- high-resolution terminology - matching measurements at high-resolution
Saponin
Description
Saponin is a mild detergent that permeabilizes plasma membranes completely and selectively due to their high cholesterol content, whereas mt-membranes with lower cholesterol content are affected only at higher concentrations. Applied for permeabilization of muscle fibres.
Abbreviation: Sap
Reference: Doerrier 2018 Methods Mol Biol
MitoPedia topics: Permeabilization agent
Application in HRR
Sap: Saponin (from quillaja bark); Sigma S7900, 25 g, store at RT.
Comment: Saponin from Quillaja Bark has the product numbers S7900 and S2149, but S2149 is withdrawn from Sigma.
Caution: Prepare fresh everyday!
Preparation of 5 mg/mL stock solution (dissolved in BIOPS):
- 1) Weigh 5 mg of saponin.
- 2) Dissolve in 1 mL BIOPS.
→ Use 50 µg saponin/mL BIOPS: add 20 µL of 5 mg/mL saponin stock solution per 2 mL BIOPS.
