Difference between revisions of "SUIT-008 O2 pfi D014"
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Revision as of 17:28, 21 January 2019
- high-resolution terminology - matching measurements at high-resolution
SUIT-008 O2 pfi D014
Description
Abbreviation: NS(PGM)
Reference: A: SUIT-008 protocol in MitoEAGLE project (WG2) for testing the skills preparing permeabilized fibers Lemieux 2017 Sci Rep
SUIT number: D014_1PM;2D;2c;3G;4S;4D;5U;6Rot;7Ama;8AsTm;9Azd
O2k-Application: O2
- SUIT-category: NS(PGM)
- SUIT protocol pattern: diametral 1PM;2D;3G;4S;5U;6Rot
References
| Year | Reference | Organism | Tissue;cell | |
|---|---|---|---|---|
| Votion 2012 PLoS One | 2012 | Votion DM, Gnaiger E, Lemieux H, Mouithys-Mickalad A, Serteyn D (2012) Physical fitness and mitochondrial respiratory capacity in horse skeletal muscle. PLoS One 7:e34890. | Horse | Skeletal muscle |
| Step | Respiratory state | Pathway control | Entry through electron transfer Complex | Comments |
|---|---|---|---|---|
| 1PM | PM(L) | N | CI | |
| 2D | PM(P) | N | CI | |
| 2D(2c) | PM(c) | N | CI | |
| 3G | PGM(P) | N | CI | |
| 4S | PGMS(P) | NS | CI&II | |
| 5U | PGMS(E) | NS | CI&II | |
| 6Rot | S(E) | S | CII | |
| 7Ama | ROX |
1PM;2D;3G;3c;4S;5U;6Rot-
1PM;2D;3G;3c;4S;5U;6Rot;7Ama
| Step | Respiratory state | Pathway control | Entry through electron transfer Complex | Comments |
|---|---|---|---|---|
| 1PM | PM(L) | N | CI | |
| 2D | PM(P) | N | CI | |
| 3G | PGM(P) | N | CI | |
| G(c) | PGM(c) | N | CI | Limitation: Cytochrome c should be added after step 2D, such that all OXPHOS states can be compared in the presence of cytochrome c. See 1PM;2D;2c;3G;4S;5U;6Rot-. |
| 4S | PGMS(P) | NS | CI&II | |
| 5U | PGMS(E) | NS | CI&II | |
| 6Rot | S(E) | S | CII | |
| 7Ama | ROX |
References
| Year | Reference | Organism | Tissue;cell | |
|---|---|---|---|---|
| Lemieux 2017 Sci Rep | 2017 | Lemieux H, Blier PU, Gnaiger E (2017) Remodeling pathway control of mitochondrial respiratory capacity by temperature in mouse heart: electron flow through the Q-junction in permeabilized fibers. Sci Rep 7:2840. doi:10.1038/s41598-017-02789-8 | Mouse | Heart |
MitoPedia concepts:
SUIT protocol,
SUIT A,
Find
MitoPedia methods:
Respirometry
