Difference between revisions of "SUIT-003 O2 ce D009"
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|description=[[File:Ce R 1Omy 2U 3RotAma.png|right|200px]] | |description=[[File:Ce R 1Omy 2U 3RotAma.png|right|200px]] | ||
'''[[Categories of SUIT protocols |SUIT-catg]]:''' CC | '''[[Categories of SUIT protocols |SUIT-catg]]:''' CC | ||
|info=[[MiPNet08.09 CellRespiration]] | |info=[[MiPNet08.09 CellRespiration]] | ||
}} | }} | ||
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|mitopedia concept=SUIT protocol | |mitopedia concept=SUIT protocol | ||
}} | }} | ||
::: '''[[SUIT protocol pattern]]:''' linear [[coupling control protocol]] (CCP) | |||
::: '''[[Mark names in DatLab]]:''' MiPNet08_09_CellRespiration | |||
::: '''[[DatLab-Excel templates |DatLab-Excel template]]:''' [[Media:SUIT MiPNet08.09 CellRespiration.xlsx|SUIT_MiPNet08.09_CellRespiration.xlsx]] | |||
:::* '''Rot and Ama''' | :::* '''Rot and Ama''' | ||
:::: In this CCP, ROX is measured after titration of [[rotenone]], [[antimycin A]], or both in a single step. Validity of using [[rotenone]] only for inducing ROX in intact cells is evaluated by stepwise measurement of ROX after titration of rotenone (3Rot) followed by titration of animycin A (4Ama). | :::: In this CCP, ROX is measured after titration of [[rotenone]], [[antimycin A]], or both in a single step. Validity of using [[rotenone]] only for inducing ROX in intact cells is evaluated by stepwise measurement of ROX after titration of rotenone (3Rot) followed by titration of animycin A (4Ama). | ||
::::» ''Further details'': [[CCP Huetter 2004 Biochem J]], Ce_R 1Omy 2U 3Rot 4Ama | ::::» ''Further details'': [[CCP Huetter 2004 Biochem J]], Ce_R 1Omy 2U 3Rot 4Ama | ||
Revision as of 14:13, 29 August 2016
- high-resolution terminology - matching measurements at high-resolution
SUIT-003 O2 ce D009
Description
SUIT-catg: CC
Abbreviation: CC_R 1Omy 2U 3Ama
Reference: MiPNet08.09 CellRespiration
MitoPedia concepts:
SUIT protocol
- SUIT protocol pattern: linear coupling control protocol (CCP)
- Mark names in DatLab: MiPNet08_09_CellRespiration
- DatLab-Excel template: SUIT_MiPNet08.09_CellRespiration.xlsx
- Rot and Ama
- In this CCP, ROX is measured after titration of rotenone, antimycin A, or both in a single step. Validity of using rotenone only for inducing ROX in intact cells is evaluated by stepwise measurement of ROX after titration of rotenone (3Rot) followed by titration of animycin A (4Ama).
- » Further details: CCP Huetter 2004 Biochem J, Ce_R 1Omy 2U 3Rot 4Ama
