Difference between revisions of "Remels 2010 Mol Cell Endocrinol"
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{{Publication | {{Publication | ||
|title=Remels AH, Langen RC, Schrauwen P, Schaart G, Schols AM, Gosker HR (2010) Regulation of mitochondrial biogenesis during myogenesis. Mol Cell Endocrinol 315: 113- | |title=Remels AH, Langen RC, Schrauwen P, Schaart G, Schols AM, Gosker HR (2010) Regulation of mitochondrial biogenesis during myogenesis. Mol Cell Endocrinol 315:113-20. | ||
|info=[http:// | |info=[http://www.ncbi.nlm.nih.gov/pubmed/19804813 PMID:19804813] | ||
|authors=Remels AH, Langen RC, Schrauwen P, Schaart G, Schols AM, Gosker HR | |authors=Remels AH, Langen RC, Schrauwen P, Schaart G, Schols AM, Gosker HR | ||
|year=2010 | |year=2010 | ||
|journal=Mol Cell Endocrinol | |journal=Mol Cell Endocrinol | ||
|abstract=Pathways involved in mitochondrial biogenesis associated with myogenic differentiation are poorly defined. Therefore, C(2)C(12) myoblasts were differentiated into multi-nucleated myotubes and parameters/regulators of mitochondrial biogenesis were investigated. Mitochondrial respiration, citrate synthase- and beta-hydroxyacyl-CoA dehydrogenase activity as well as protein content of complexes I, II, III and V of the mitochondrial respiratory chain increased 4-8-fold during differentiation. Additionally, an increase in the ratio of myosin heavy chain (MyHC) slow vs MyHC fast protein content was observed. PPAR transcriptional activity and transcript levels of PPAR-alpha, the PPAR co-activator [[PGC-1alpha]], mitochondrial transcription factor A and nuclear respiratory factor 1 increased during differentiation while expression levels of PPAR-gamma decreased. In conclusion, expression and activity levels of genes known for their regulatory role in skeletal muscle oxidative capabilities parallel the increase in oxidative parameters during the myogenic program. In particular, [[PGC-1alpha]] and PPAR-alpha may be involved in the regulation of mitochondrial biogenesis during myogenesis. | |abstract=Pathways involved in mitochondrial biogenesis associated with myogenic differentiation are poorly defined. Therefore, C(2)C(12) myoblasts were differentiated into multi-nucleated myotubes and parameters/regulators of mitochondrial biogenesis were investigated. Mitochondrial respiration, citrate synthase- and beta-hydroxyacyl-CoA dehydrogenase activity as well as protein content of complexes I, II, III and V of the mitochondrial respiratory chain increased 4-8-fold during differentiation. Additionally, an increase in the ratio of myosin heavy chain (MyHC) slow vs MyHC fast protein content was observed. PPAR transcriptional activity and transcript levels of PPAR-alpha, the PPAR co-activator [[PGC-1alpha]], mitochondrial transcription factor A and nuclear respiratory factor 1 increased during differentiation while expression levels of PPAR-gamma decreased. In conclusion, expression and activity levels of genes known for their regulatory role in skeletal muscle oxidative capabilities parallel the increase in oxidative parameters during the myogenic program. In particular, [[PGC-1alpha]] and PPAR-alpha may be involved in the regulation of mitochondrial biogenesis during myogenesis. | ||
|keywords=Peroxisome, | |keywords=Peroxisome, Proliferator-activated, Receptors, Myogenesis, Mitochondrial transcription factor A, Nuclear respiratory factor 1, Skeletal muscle, C2C12 cells, L6 muscle cells | ||
|mipnetlab=NL Maastricht Schrauwen P | |mipnetlab=NL Maastricht Schrauwen P | ||
}} | }} | ||
{{Labeling | {{Labeling | ||
|area=Respiration | |||
|organism=Mouse, Rat | |||
|tissues=Skeletal muscle, Other cell lines | |||
|preparations=Permeabilized cells | |||
|couplingstates=ROUTINE, OXPHOS, ET | |||
|pathways=F | |||
|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
}} | }} | ||
Latest revision as of 15:46, 13 November 2017
| Remels AH, Langen RC, Schrauwen P, Schaart G, Schols AM, Gosker HR (2010) Regulation of mitochondrial biogenesis during myogenesis. Mol Cell Endocrinol 315:113-20. |
Remels AH, Langen RC, Schrauwen P, Schaart G, Schols AM, Gosker HR (2010) Mol Cell Endocrinol
Abstract: Pathways involved in mitochondrial biogenesis associated with myogenic differentiation are poorly defined. Therefore, C(2)C(12) myoblasts were differentiated into multi-nucleated myotubes and parameters/regulators of mitochondrial biogenesis were investigated. Mitochondrial respiration, citrate synthase- and beta-hydroxyacyl-CoA dehydrogenase activity as well as protein content of complexes I, II, III and V of the mitochondrial respiratory chain increased 4-8-fold during differentiation. Additionally, an increase in the ratio of myosin heavy chain (MyHC) slow vs MyHC fast protein content was observed. PPAR transcriptional activity and transcript levels of PPAR-alpha, the PPAR co-activator PGC-1alpha, mitochondrial transcription factor A and nuclear respiratory factor 1 increased during differentiation while expression levels of PPAR-gamma decreased. In conclusion, expression and activity levels of genes known for their regulatory role in skeletal muscle oxidative capabilities parallel the increase in oxidative parameters during the myogenic program. In particular, PGC-1alpha and PPAR-alpha may be involved in the regulation of mitochondrial biogenesis during myogenesis. • Keywords: Peroxisome, Proliferator-activated, Receptors, Myogenesis, Mitochondrial transcription factor A, Nuclear respiratory factor 1, Skeletal muscle, C2C12 cells, L6 muscle cells
• O2k-Network Lab: NL Maastricht Schrauwen P
Labels: MiParea: Respiration
Organism: Mouse, Rat
Tissue;cell: Skeletal muscle, Other cell lines
Preparation: Permeabilized cells
Coupling state: ROUTINE, OXPHOS, ET
Pathway: F
HRR: Oxygraph-2k
