Difference between revisions of "Pinho 2023 Eur J Clin Invest"

Revision as of 15:49, 21 November 2023

Pinho ACO, Santos D, Oliveira PJ, Leal EC, Carvalho E (2023) Real-time OXPHOS capacity analysis in wounded skin from diabetic mice: A pilot study. https://doi.org/10.1111/eci.14128

» Eur J Clin Invest 17:e14128. PMID: 37975307 Open Access

Pinho Aryane Cruz Oliveira, Santos Diana, Oliveira Paulo J, Leal Ermelindo Carreira, Carvalho Eugenia (2023) Eur J Clin Invest

Abstract: Diabetes mellitus (DM) impairs wound healing. The aim was to determine whether DM influences mitochondrial respiration in wounded skin (WS) and non-wounded skin (NWS), in a pre-clinical wound healing model of streptozotocin (STZ)-induced diabetes.

Six weeks after diabetes induction, two wounds were created in the back of C57BL/J6 mice. Using high-resolution respirometry (HRR), oxygen flux was measured, in WS and NWS, using two substrate-uncoupler-inhibitor titration protocols, at baseline (day 0), day 3 and 10 post-wounding, in STZ-DM and non-diabetic (NDM) mice. Flux control ratios for the oxidative phosphorylation (OXPHOS) capacity were calculated.

A significant increase in mitochondrial respiration was observed in STZ-DM skin compared to control skin at baseline. The OXPHOS capacity was decreased in WS under diabetes at day 3 post-wounding (inflammation phase). However, at day 10 post-wounding (remodeling phase), the OXPHOS capacity was higher in WS from STZ-DM compared to NDM mice, and compared to NWS from STZ-DM mice. A significant relative contribution of pyruvate, malate and glutamate (PMG) oxidation to the OXPHOS capacity was observed in WS compared to NWS from STZ-DM mice, at day 10, while the relative contribution of fatty acid oxidation to the OXPHOS capacity was higher in NWS. The OXPHOS capacity is altered in WS from STZ-DM compared to NDM mice across the healing process, and so is the substrate contribution in WS and NWS from STZ-DM mice, at each time point.

HRR may be a sensitive tool to evaluate the underlying mechanisms of tissue repair during wound healing. • Keywords: OXPHOS capacity, Diabetes, Diabetic wound, High-resolution respirometry, Mitochondrial respiration, Skin • Bioblast editor: Plangger M • O2k-Network Lab: PT Coimbra Carvalho E

Labels: MiParea: Respiration Pathology: Diabetes

Organism: Mouse

HRR: Oxygraph-2k

2023-11

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 |keywords=OXPHOS capacity, Diabetes, Diabetic wound, High-resolution respirometry, Mitochondrial respiration, Skin
 |editor=[[Plangger M]]
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