Difference between revisions of "Osakai 2019 Electrochemistry"
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|abstract=The redox behavior of cytochrome ''c'' (Cyt ''c'') at a ubiquinone-10 (UQ) incorporated self-assembled monolayer (SAM)-modified electrode was studied by cyclic voltammetry. A well-defined catalytic wave due to the reduction of Cyt ''c'' by UQ was observed at around −0.4 V vs. Ag/AgCl (saturated KCl). However, the re-oxidation peak of UQ at around +0.3 V was small, suggesting no significant catalytic ability of UQ for the re-oxidation of Cyt ''c''. These voltammetric behaviors could be well simulated by digital simulation with a simple reaction model in which UQ and Cyt ''c'' coexist homogeneously in a reaction layer on the base gold electrode. The parameters obtained by curve fitting of cyclic voltammograms showed that the re-oxidation of Cyt ''c'' by UQ is somewhat thermodynamically unfavorable and, importantly, kinetically slow. This slow process is probably originated from spatial separation between the redox species. Such a directional or one-way electron transfer may be occurring in the mitochondrial respiratory chain system to achieve efficient energy production. | |abstract=The redox behavior of cytochrome ''c'' (Cyt ''c'') at a ubiquinone-10 (UQ) incorporated self-assembled monolayer (SAM)-modified electrode was studied by cyclic voltammetry. A well-defined catalytic wave due to the reduction of Cyt ''c'' by UQ was observed at around −0.4 V vs. Ag/AgCl (saturated KCl). However, the re-oxidation peak of UQ at around +0.3 V was small, suggesting no significant catalytic ability of UQ for the re-oxidation of Cyt ''c''. These voltammetric behaviors could be well simulated by digital simulation with a simple reaction model in which UQ and Cyt ''c'' coexist homogeneously in a reaction layer on the base gold electrode. The parameters obtained by curve fitting of cyclic voltammograms showed that the re-oxidation of Cyt ''c'' by UQ is somewhat thermodynamically unfavorable and, importantly, kinetically slow. This slow process is probably originated from spatial separation between the redox species. Such a directional or one-way electron transfer may be occurring in the mitochondrial respiratory chain system to achieve efficient energy production. | ||
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{{Labeling | {{Labeling | ||
|topics=Q-junction effect | |topics=Q-junction effect | ||
|additional=MitoFit 2021 CoQ | |additional=MitoFit 2021 CoQ | ||
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Latest revision as of 12:20, 6 May 2021
| Osakai T, Yamamoto T, Ueki M (2019) Directional electron transfer from ubiquinone-10 to cytochrome c at a biomimetic self-assembled monolayer modified electrode. Electrochemistry 87:59-64. |
Osakai T, Yamamoto T, Ueki M (2019) Electrochemistry
Abstract: The redox behavior of cytochrome c (Cyt c) at a ubiquinone-10 (UQ) incorporated self-assembled monolayer (SAM)-modified electrode was studied by cyclic voltammetry. A well-defined catalytic wave due to the reduction of Cyt c by UQ was observed at around −0.4 V vs. Ag/AgCl (saturated KCl). However, the re-oxidation peak of UQ at around +0.3 V was small, suggesting no significant catalytic ability of UQ for the re-oxidation of Cyt c. These voltammetric behaviors could be well simulated by digital simulation with a simple reaction model in which UQ and Cyt c coexist homogeneously in a reaction layer on the base gold electrode. The parameters obtained by curve fitting of cyclic voltammograms showed that the re-oxidation of Cyt c by UQ is somewhat thermodynamically unfavorable and, importantly, kinetically slow. This slow process is probably originated from spatial separation between the redox species. Such a directional or one-way electron transfer may be occurring in the mitochondrial respiratory chain system to achieve efficient energy production.
Cited by
- Komlódi T, Cardoso LHD, Doerrier C, Moore AL, Rich PR, Gnaiger E (2021) Coupling and pathway control of coenzyme Q redox state and respiration in isolated mitochondria. Bioenerg Commun 2021.3. https://doi.org/10.26124/bec:2021-0003
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Regulation: Q-junction effect
MitoFit 2021 CoQ
